Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEX-4T-3-Rab13

Expression vector of mouse Rab13 tagged with GST at N-terminus, tac promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. J. Biol. Chem. 278, 15373-15380, 2003); (Itoh, T. et al. Genes Cells 11, 1023-1037, 2006)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16838_A9A8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403) Region: GST,thrombin site,insert Sequence file: RDB16838_A9A8a.seq
>16838_16838_A9A8_pGEX-F2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNTNNNNNN NNNTATTGAA GCTNTCCNNC AAATTGATAA GTACTTGAAA    61 TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC   121 CATCCTCCAA AATCGGATCT GGTTCCGCGT GGATCCATGG CCAAAGCCTA CGACCACCTC   181 TTCAAGTTGC TGCTCATCGG GGACTCGGGG GTGGGCAAGA CTTGTCTGAT CATTCGCTTT   241 GCAGAGGACA ACTTCAACAG CACTTACATC TCTACCATCG GAATTGATTT CAAGATCCGA   301 ACCGTGGACA TAGAGGGGAA GAGGATCAAA CTGCAAGTGT GGGACACGGC TGGCCAAGAA   361 CGATTCAAGA CAATAACTAC CGCCTATTAC CGTGGAGCCA TGGGCATTAT CCTCGTATAT   421 GACATCACAG ATGAGAAATC CTTCGAGAAT ATTCAGAACT GGATGAAAAG CATCAAAGAG   481 AATGCCTCTG CGGGAGTGGA GCGCCTCCTG CTGGGAAACA AGTGTGACAT GGAGGCCAAG   541 CGGCAGGTGC AGAGAGAGCA GGCGGAGAAG TTGGCTCGAG AGCACAGAAT CCGATTTTTT   601 GAGACGAGTG CCAAATCCAG TGTGAATGTG GATGAGGCTT TCAGTTCCCT GGCCCGTGAC   661 ATCTTGCTCA AGACAGGAGG CCGGAGATCG GGAACCAACA GTAAGCCCTC AAGCACTGGC   721 CTGAAAACAT CTGACAAGAA GAACAACAAG TGCTTGTTAG GCTGAAATCA CTAGTGAATT   781 CGCGGCCGCA TCGTGACTGA CTGACGATCT GCCTCGCGCG TTTCGGTGAN GACNGNGAAA   841 ACCTCTGACA CATGCAGCTC CCGGAGANGG NCACAGCTNG TCTGTAAGCG GATGNCGGGA   901 NCAGANAAGC CCGNCANGNN GCGNCAGCNG NNGTTGNCGG NNGNCNGGGC GCANCCNTNN 961 NCCANNCNCN TANCGATAGC NNANNGNATA NNTCNNGNNN NNNANNGNNN NNNNNNN //
Primer: pGEX-ptac_up-F (Pr0455) Region: tac pro,lacO,GST Sequence file: RDB16838_A9A8b.seq
>16838_16838_A9A8_pGEX-ptaq_up_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NTCTGNNNNN NTTCTGAAAT GAGCTGTTGA CNATTAATCA    61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT   121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT   181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA   241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA   301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA   361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT   421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT   481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA   541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA   601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA   661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC   721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGNTGGC GACCATCCTC CNAAATCGGA   781 TCTGGTTCCG CGTGGATCCN TGGCCNAAGC CTACGACCAC CTCTTCNAGT TGCTGCTCAT   841 CGGGGANTCN GGGGTGGGCA AGANTTGTCT GANCATTCGC TTTGCANANG ANAACNNCAN   901 CNGCNCTTAC ATCTCTACNN TCNNANNTGA NTTCNNGATN CNAACCGNNN NNNTANAGGG   961 GAAGANNNNC AAANNGCANN NGTGGNNNNN NGNNNGNNNN NNANNNANNC NNNNNNNNNN //
Primer: pBAC_lac_F (Pr0544) Region: lacI Sequence file: RDB16838_A9A8c.seq
>16838_16838_A9A8_pBAC_lac_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC CGNANNNTGG TCNTAGCTGT TTCCTGTNNT GAAATTGTTA    61 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC   121 CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG   181 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG   241 TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT   301 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC   361 GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT   421 CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA   481 TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT   541 TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG   601 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG   661 CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA   721 GATGCTCCAC GCCCAGTCGC GTANCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG   781 TCTGGTCAGA GACATCNAGA AATAACGCCN GANCATTAGT GCAGGCAGCT TCCACAGCAA   841 TGGCATCCTG NTCATNCNGC GGATAGTTAA TGATCNGCCC ANNGACGCNT TGCGCGAGAA   901 GANTGNGCAC CGCCGCTTTA CAGGCTTCNA NGNCGNTTCN TTCTACCNTC GANNNNNNNN 961 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl