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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human TLR8 expression vector (#RDB16910)

Expression vector of human TLR8.

Clone info. Expression vector of human Toll-like receptor 8 (TLR8).
Comment Complementary DNAs for human TLR8 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Please look at AAZ95441 as a reference. Expression was confirmed by the depositor with western blotting with anti-TLR8 Ab.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR8 cDNA
Depositor|Developer Matsumoto, Misako |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, H et al. PLoS One. 6(12) : e28500, 2011); (Ishii, N et al. J. Immunol. 193(10) : 5118-5128, 2014)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16910 human TLR8 expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TLR8 expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16910).

Reference section:

Itoh, H., Tatematsu, M., Watanabe, A., Iwano, K., Funami, K., Seya, T., Matsumoto, M., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16910_A9Ajp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Region: EF1alpha pro,insert5'
Sequence file: RDB16910_A9Aja.seq check
>D06031A2_A9Aj_2_EF1a-C_F_D04_10_ABI24.ab1
    1 CCATGGATTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGATC CCTCGACCTC
  121 GAGGCCACCA TGAAGGAGTC ATCTTTGCAA AATAGCTCCT GCAGCCTGGG AAAGGAGACT
  181 AAAAAGGAAA ACATGTTCCT TCAGTCGTCA ATGCTGACCT GCATTTTCCT GCTAATATCT
  241 GGTTCCTGTG AGTTATGCGC CGAAGAAAAT TTTTCTAGAA GCTATCCTTG TGATGAGAAA
  301 AAGCAAAATG ACTCAGTTAT TGCAGAGTGC AGCAATCGTC GACTACAGGA AGTTCCCCAA
  361 ACGGTGGGCA AATATGTGAC AGAACTAGAC CTGTCTGATA ATTTCATCAC ACACATAACG
  421 AATGAATCAT TTCAAGGGCT GCAAAATCTC ACTAAAATAA ATCTAAACCA CAACCCCAAT
  481 GTACAGCACC AGAACGGAAA TCCCGGTATA CAATCAAATG GCTTGAATAT CACAGACGGG
  541 GCATTCCTCA ACCTAAAAAA CCTAAGGGAG TTACTGCTTG AAGACAACCA GTTACCCCAA
  601 ATACCCTCTG GTTTGCCAGA GTCTTTGACA GAACTTAGTC TAATTCAAAA CAATATATAC
  661 AACATAACTA AAGAGGGCAT TTCAAGACTT ATAAACTTGA AAAATCTCTA TTTGGCCTGG
  721 AACTGCTATT TTAACAAAGT TTGCGAGAAA ACTAACATAG AAGATGGAGT ATTTGAAACG
  781 CTGACAAATT TGGAGTTGCT ATCACTATCT TTCAATTCTC TTTCACACGT GCCACCCAAA
  841 CTGCCAAGCT CCCTACGCAA ACTTTTTCTG AGCAACACCC AGATCAAATA CATTAGTGAA
  901 GAAGATTTCA AGGGATTGAT AAATTTAACA TTACTAGATT TAAGCGGGAA CTGTCCGAGG
  961 TGCTTCAATG CCCCATTTCC ATGCGTGCCT TGTGATGGTG GGTGCTTCAA TTAATATAGA
 1021 TCGTTTTGCT TTTCAAAACC TTGGACCCAA CTTTCGATAC CCTAAACCCT CTCTAGCCAC
 1081 TTTCCCTTCA GGAGATAATG CTGCCTGGAT AAAATATGCT TCAATCTGAG GTGGCTGCAT
1141 CTTGCAATTC CAACCTAATT TTAAGGTGGG GGGAGA
//
Primer: hGCSF_R ()
Region: hGCSF p(A),insert3'
Sequence file: RDB16910_A9Ajb.seq check
>D06031A2_A9Aj_2_hGCSF_R_E04_13_ABI24.ab1
    1 CTGGGGAAAA TGATCTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGAG TCGCGGCCGC
   61 CGCGAAATCA TGACTTAACG TCAGTTAGTA TTGCTTAATG GAATCGACAT ACATATTGTT
  121 ATACCGTGAA TCATTTTCAG TCAAGACCAC ATTTCTCAGA GTTTGCCAAA ACAAGCCTTC
  181 TGCCTTCGGG TTGTCAGGCC ACTGGAGGAT GGAGCTCTTA CAGATCCGCT GCCGTAGCCT
  241 CAAATACTGA GAATGCTGTA ACACTGGCTC CAGCAGGATA AATATAATCA CATCCATGTT
  301 CTCATCCATT AGCCTCTGCA AAGCCAAGTA AAAAGCTGTT TTAAAGTTCC AGCTTTTTGC
  361 ATATTTTTTG GTTAAAACAA ATACTGTTTT CTTGCTTTGG TTGATGCTCT GCATGAGGTT
  421 GTCGATGATG GCCAATCCCG GGTCCCAATC CCTCTCCTCT AGACAAAGGA GAACGTTTTT
  481 GTCTCGGCTC TCTTCAAGGT GGTAGCGCAG CTCATTTATC ACCCAGTCAG TAACAGAGGC
  541 ATCTTTGGTG TCATAAGAAA TGTAAGCATC ATAGAAAGTT TGGGATGTGG AAAGAGACCT
  601 GTAGCCTTTT ACCTTAGCTA AACACACATT ATATATAAAC CAAACATCCC AGTAAAACAA
  661 ATGGTGAGCC AGGGCAGCCA ACATAACCAT GGTGGTGATA AAGAACGTGA AGAAAAATAA
  721 TATCACTGCA GTGACATCTG AAACACAAGT TGTTAGCTCC AGACTCACAA TACTCTTCCC
  781 TCTTTGATCC CCAGGACTGG CACAAATGAC ATCTACCAGT CTGGGAATTT TGACATTCAG
  841 ATGTTCATCC ATCCATCTTC GGAAATCTCC AATGTCACAG GTGCATTCAA AGGGGTTTCC
  901 GTGTAGTTCC AACATAGATA ATTTGGTGGT GGTCTTAGTT TCAAGTGCGG ATTTGTTTAT
  961 TGTTTTTAGC AGATTGGGAG ACTTAAATCG AGGTGCTTCA GACTACTGAC TTCAGAAAGA
 1021 AAGCCAGAGG GTAGGTGGGG AAATCCTTGT TATGACTCAG CAGCAGTGTC CGAAAGGGAA
 1081 GATGTAAAGT TCAGATAGCT ATCAGTTAAA AGAGTAGGTG ATCACGGTAG TTCAAGCCAA
1141 CTCGAGACGA GGAACTGCTG GAATAATGTT TCCAGAGTTA TA
//
Primer: reverse ()
Region: SV40pro_ori,EF1alpha pro
Sequence file: RDB16910_A9Ajc.seq check
>D06031A2_A9Aj_2_reverse_F04_16_ABI24.ab1
    1 CAATATTTCC TCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
   61 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
  121 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
  181 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT
  241 TGCAAAGATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG
  301 TCTTGAAAGG AGTGGGAATT GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA
  361 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC
  421 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG
  481 GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG
  541 CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG
  601 GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC
  661 TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC
  721 GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC
  781 TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG
  841 CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG
  901 GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT
  961 CGGCGAGGCG GGGCCTGCGA GCGCGGGCCA CCGAGAAATC GGACGGGGGG TAGTCTCAAG
 1021 CTGGGCCGGG CCTGCTCTGG GTGCCTGGAC CTCGCGCCGT CGTGTATCGC CCGCCTGAGC
 1081 GGCAAGGCTG TCTGGTCGGC ACCAGTTGCG TGAACGGAAG ATGACGGCAT TCCCGGGCCT
1141 GCTGCAGGGG AAGCTCTCAA AATGTGGAAG GA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, H., UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One 6 (12): e28500 (2011). PMID 22164301. [link to RRC of NBRP]