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human TLR3-CFlag expression vector (#RDB16911)

Expression vector of human TLR3 tagged with FLAG at C-terminus.

Clone info. Expression vector of human Toll-like receptor 3 (TLR3) tagged with FLAG at C-terminus.
Comment Complementary DNAs for human TLR3 was cloned by RT-PCR from the mRNA of monocyte-derived immature DCs. Expression was confirmed by the depositor with immunoprecipitation and western blotting.
Vector backbone pEFBOS (plasmid)
Selectable markers Amp^r
Gene/insert name human TLR3 cDNA
Depositor|Developer Matsumoto, Misako |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsumoto, M et al. Biochem. Biophys. Res. Commun. 293(5) : 1364-1369, 2002); (Matsumoto, M et al. Int. Immunol. 16(8) : 1143-1154, 2004)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16911 human TLR3-CFlag expression vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human TLR3-CFlag expression vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16911).

Reference section:

Matsumoto, M., Kikkawa, S., Kohase, M., Miyake, K., Seya, T., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16911_A9Ajp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro,insert 5'(TLR3 5')
Sequence file: RDB16911_A9Ajd.seq check
>D06031A3_A9Aj_2_EF1a-C_F_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNTTTTGAGT TTGGNNCTTG GTTCNTTCTC NAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AGGAATTCTC TAGAGATCCC TCGACCTCGA
  121 GGCCGCCACC ATGAGACAGA CTTTGCCTTG TATCTACTTT TGGGGGGGCC TTTTGCCCTT
  181 TGGGATGCTG TGTGCATCCT CCACCACCAA GTGCACTGTT AGCCATGAAG TTGCTGACTG
  241 CAGCCACCTG AAGTTGACTC AGGTACCCGA TGATCTACCC ACAAACATAA CAGTGTTGAA
  301 CCTTACCCAT AATCAACTCA GAAGATTACC AGCCGCCAAC TTCACAAGGT ATAGCCAGCT
  361 AACTAGCTTG GATGTAGGAT TTAACACCAT CTCAAAACTG GAGCCAGAAT TGTGCCAGAA
  421 ACTTCCCATG TTAAAAGTTT TGAACCTCCA GCACAATGAG CTATCTCAAC TTTCTGATAA
  481 AACCTTTGCC TTCTGCACGA ATTTGACTGA ACTCCATCTC ATGTCCAACT CAATCCAGAA
  541 AATTAAAAAT AATCCCTTTG TCAAGCAGAA GAATTTAATC ACATTAGATC TGTCTCATAA
  601 TGGCTTGTCA TCTACAAAAT TAGGAACTCA GGTTCAGCTG GAAAATCTCC AAGAGCTTCT
  661 ATTATCAAAC AATAAAATTC AAGCGCTAAA AAGTGAAGAA CTGGATATCT TTGCCAATTC
  721 ATCTTTAAAA AAATTAGAGT TGTCATCGAA TCAAATTAAA GAGTTTTCTC CAGGGTGTTT
  781 TCACGCAATT GGAAGANTAT TTGGCCTCTT TCTGAACAAT GTCCAGCTGG GTCCCAGCCT
  841 TACAGANAAN CTATGTTTGG AATTAGCAAA CACAAGCATT CGGAATCTGT CTCTGANTAA
  901 CAGCCAGCTG TCCNCCACCA GNNATACNAC TTTCTNGGNN NTNAAGTGGN NAAATCTCAC
  961 TANGCTCNNT CTTTCCNANN NCNACTTAAA NGNGGNNGNN NNCGANNNCN TNGNNNNNNN
1021 TCCNNNNNNA NNNNNNTTNN NNNCNNNNNN NNNN
//
Primer: hGCSF_R (Pr0031)
Region: insert 3'(FLAG,TLR3 3')
Sequence file: RDB16911_A9Aje.seq check
>D06031A3_A9Aj_2_hGCSF_R_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN GGTCNNNGGT GGGGNNCCTC ACTCTNNGAG TCGCGGCCGC
   61 TTACTTGTCA TCGTCGTCCT TGTAGTCATG TACAGAGTTT TTGGATCCAA GTGCTACTTG
  121 CAATTTATGG CGAAAGGCAC CTATCCGTTC TTTCTGAACT GGCCAGTTCA AGATGCAGTG
  181 AGATTTAAAC ATTCCTCTTC GCAAACAGAG TGCATGGTTC AGTTTATAAT CTGGAATCTC
  241 CTCAAGGAAA ACCAATATAA TGGAATCCAG ATTTTGTTCA ATAGCTTGTT GAACTGCATG
  301 ATGTACCTTG AATCTTTTGC ATAATGGGTC TTTTAATAGA TGGTGTGTTA TAACAAAAAT
  361 AATTTTTCTG CTTCTTTTGA TGCTGTTAAC AATTGCTTCT AGTTCAAAAA CACCCGCCTC
  421 AAAGTCCCTT TCTTCCAGAC AAAATTTGAG AGATTGGTCT TCCTTTTCCA TTGAAGAGAA
  481 ATGTTCCCAG ACCCAATCCT TATCTTTATA GGCATGAATT ATATATGCTG CATATTCAAA
  541 CTGTTCTGTC TGTCTGTCTA TTTCTTTGAA ACCAAGAACT CGATGTACTG AAACATTCCA
  601 ATAAAAAGAT ATCCTCCAGC CCTCAAAGTG GATGAGAAGT ACAATAAAGA TAAAAATCAA
  661 CAGGATACTG GTATTGATCA TGAAAAAGAG TTCAAAGGGG GCACTGTCTT TGCAAGATGA
  721 TGTATCAAAA AGTCTCACTG GGAACCCATG ATAGTGAGGT GGAGTGTTGC AAANNNAGTG
  781 GCTTGACAGC TCAGGGATGT TGGTATGGGT CTCGTTAATC NNATTAACAA ACCAGGCAAT
  841 ACTTTCACAC GTGCAATCAA AGGGANTAAA GCGCATATCT NACTCNGTCA GGNTNCTGAA
  901 AGCTGGNCCG AAAANNTTCT NCTCNNCNNA TGTTATGANA NNCTTCTGAA NNNTNANTGN
  961 NTTTNNAGAN NNCTGANTAT TAAAGANAGA TGCTGGNANN GTGTTTNAAN TNATTNNNTC
1021 CNAAATNGAN GATNNTTANN NNNNNNANNN CNNNNNNNNN N
//
Primer: reverse (Pr0099)
Region: SV40 pro_ori,EF-1 alpha pro
Sequence file: RDB16911_A9Ajf.seq check
>D06031A3_A9Aj_2_reverse_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNANGCA GAAGTANGCN AAGCATGCAT CTCNATTAGT CAGCAACCAT
   61 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC
  121 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA
  181 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTTG
  241 CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC
  301 TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA
  361 GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC
  421 GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA
  481 GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC
  541 AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC
  601 CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT
  661 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT
  721 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT
  781 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCNTTT AAAATTTTTG ATGACCTGCT
  841 GCGACGCTTT TTTTCTGGCN AGATAGTCTT GTAAATGCGG GCCNAGATCT GNACACTGGT
  901 ATTTCGGNTT TTGGGGNCGC GGGCGGCGAN NGGGCCNNNN CGNNCCNNCG CNCATGTTCN
  961 GNGANNNGGG GNCNGCNANC NNGGNNNNNN ANNNNNNNNN GGGGNNANNN NNNNNCNNGN
1021 NNNGNNNNGN NNNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsumoto, M., Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem. Biophys. Res. Commun. 293 (5): 1364-1369 (2002). PMID 12054664. [link to RRC of NBRP]