RDB17232 - pEGFP-C1-KIF13A-tail-deltaRHD

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pEGFP-C1-KIF13A-tail-deltaRHD

Expression vector of mouse KIF13A fused with EGFP. The KIF13A-tail mutant without a motor domain and the lacks an RHD, a Rab10-binding dificient mutant.

Catalog number	RDB17232
Resource name	pEGFP-C1-KIF13A-tail-deltaRHD
Clone info.	Expression vector of mouse kinesin family member 13A (KIF13A) fused with EGFP at N-terminus. The KIF13A-tail mutant without a motor domain and the lacks an Rab10-binding homology domain (RHD), a Rab10-binding dificient mutant, see Fig. 4A and 5C of Etoh, K. and Fukuda, M., J. Cell Sci., 132 (5), jcs226977, 2019.
Comment	Expression was confirmed by the depositor with immunoblotting.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name	mouse Kif13a cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Other clones in our bank

mouse Kif13a

External Database
mouse Kif13a

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishida, M. et al. (2015) Sci. Rep. 5, 8238); (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB17232	pEGFP-C1-KIF13A-tail-deltaRHD	DNA solution

Please review the QC test results indicated by check

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References and tips

Original reference

original	Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17232_A9Elp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3', insert 5' Sequence file: RDB17232_A9Ela.seq
>D06280B5_A9El_1_EGFP-C2_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNCGC NNNCNCNTGN NCCTGCTGGN NTTCGTGNNC GCCGCCGGGN 61 TCNCTCTCGG CATGGNCGAN CTGTACAAGT CCGGACTCAG ATCCGACCCC AATGCCAAGG 121 TCATCCGAGA GCTTCGAGAG GAAGTGGAGA AACTGAGGGA GCAGCTCTCG CAGGCTGAGG 181 CCATGAAGGC CCCCGAGCTA AAGGAGAAGC TCGAAGAGTC TGAGAAGCTG ATAAAGGAGC 241 TAACGGTGAC GTGGGAAGAG AAGCTGAGGA AAACAGAAGC GATCGCACAG GAGAGACAGC 301 GGCAGCTGGA AAGCATGGGA ATCTCCCTGG AGACCTCTGG CATCAAGGTG GGGGACGACA 361 AGTGCTACCT CGTCAACCTG AACGCAGACC CTGCCCTCAA CGAGCTTCTG GTTTACTATT 421 TAAAGGATCA TACCCGGGTG GGTGCAGATA CCTCTCAGGA CATCCAGCTC TTCGGAATAG 481 GGATTCAGCC CGAGCACTGT GAGATCGACA TCGCAGCGGA TGGAGACATC ACTCTCACGC 541 CCAAAGAAAA TGCAAGGTCC TGTGTCAATG GCACCCTTGT CTGCAGTACT ACCCAGCTGT 601 GGCACGGCGA CAGGATCTTA TGGGGAAACA ACCACTTTTT TAGAATTAAC TTGCCTAAGA 661 GGAAACGGCG GGACTGGTTG AAAGACTTTG AGAGAGAAAN CAGTTCCGCG GAGCACGATC 721 TAGACGCTGC GAGCGANGCT TCCTCGGANC CNGACTACNA CTACGANTTC GCACAGATGG 781 ANGTCATCAT GAAAACACNG AACAGCANTG NTNCAGTTCA AAANGTGGTT CANGTCCNNN 841 NNAANCANTA CCNNNNNNNN NN //
Primer: SV40poltA_R2 (Pr0623) Region: insert 3' Sequence file: RDB17232_A9Elb.seq
>D06280B5_A9El_1_SV40polyA_R2_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNGNNN NNNACAAGTT AACAACAACA ATTGNATTCA TTTTATGTTT 61 CAGGTTCAGG GGGNGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT 121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC 181 CTGCAGGCGG CCGCGGGAAT TCGATTTTAC CGAGTGGCTG TGGCTTCCTG GTCGTTCTCT 241 ATCTTGGGAT GCAGCCGGTC GGCATCTGGA GGTCTTTCTG GGATACCTTT ATGGTCTGTT 301 TCGTTTGTTA GAGCCCTCCT CCTGGAGGGC TCCCCTACAG AACAATCTGC CAAACCATCA 361 AAGTCTTTGC CATCAGAAAC ACCCATGAAC TCTGTGAAGG AATGGTCCTC CAAAATGTTT 421 GTAGTGTGTT CTATCGTCAC CTCTCCTGGG CAGAATTCTC TGGCTTGGTG GCCATCTTTG 481 CTGGGGCCGA CATCCAGTTC TGAACATGAA GCAGTCCTGC ATGGCATTCT GGAACTTTCC 541 TCAGGGATGC TCTGGGGTGA CGGGGTCCCC TTGGGTAAGG CACTGTTTTC CTCCAGCGGC 601 ACAGCGATTG TCTCATCCTT CCCTGAGCCT CTTCCTACTT CTGTAAGCTC CTGATTCGAA 661 GCTGGTCTGA CATCCGGTGC AAGTGATGGT CCCGGGGGCT CGGCGCACTC GACCTCCTTT 721 GTCGAGACGG CAAGCTGGTC TGANCTGTCG CTNGNNGGGA NTGCCATGTC AGACAGAGTG 781 NCATTGGANG CACTGNNGGA NAAGTANCCA CTNGNGNNGC TGCTGGNNGN ANGGCNACGG 841 GNNANNNCTT NNTNNNNNNN TNTNGANTCT GNCNNNTCCN CCTTANAANN ANGNNNNNTC 901 CNNNNTNNTN CNANNNNNNN NNN //
Primer: mKIF12A_F1 (Pr0883) Region: insert mid Sequence file: RDB17232_A9Elc.seq
>D06280B5_A9El_1_mKIF13A_F14_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TGGCTGCGTG NNCGCCNGGT CCNCCAAGCT GCAGAGAGGG 61 CTGGACAGCT ACCAGAGAGA TGAGGAGGAT GGCGGTGATA TGGATAGTTA TCAGGAAGAA 121 GACTTGAATT GTGTAAGAGA GAGATGGTCC GATGCAGTGC TGGTGCCCGC CCCTGGCAGC 181 GGAATCCCCG GGGCACCTGC TGACTGGGTC CCACCTCCTG GCATGGAAAC ACACATCCCG 241 GTTCTCTTCC TGGATCTGAA CGCGGATGAC CTCAGTGCCA ATGAGCAGCT GGTGGGCCCC 301 CATGCGTCCG GTGTGAACTC CATCCTGCCC AAGGAACACG GCAGTCAGTT TTTCTACCTG 361 CCCATCATAA AGCACAGTGA TGACGAGGTT TCAGCCACAG CCTCCTGGGA CTCCTCTGTG 421 CACGACTCTC TTCACCTGAA TCGGGTCACA CCGCAGAATG AAAGGATCTA CCTGATCGTG 481 AAGACCACCG TGCAGCTCAG CCACCCGGCC GCCATGGAGC TAGTGTTGCG GAAGCGGATC 541 GCGGCCAATA TTTATAACAA GCAGAGTTTC ACCCAGAGTT TGAAGAGACG AATATCACTG 601 ATCAATATAT GTTACTCCTG CGGCGTGACC TATGAGATAG TATCCAATAT CCCAAAGGCG 661 ACCGAGGAGA TTGAGGANCG GGAAACCCTG GCCCTCCTGG CAGCGCGGAG TGAGAACGAG 721 GGCACTTTGG ATGGGGAGAC ATACATCGAG AAGTACACGC GGGGCGTGCT GCAGGTGGAG 781 AATATCCTGA NCCTGGANCG GCTCCGGCAN GCTGTCACGG TGAANGAAGC GCTTTCCNCC 841 AAAGCGAGGC ACATTCNGAN AANCCTCAGT ACANCGAACG TTCACNACGT CNCCTCTANC 901 CNGNCNGATN NCTCNGGNTT CGATGAAGAN NANNNNNNCT GGNCNNANAN NNANNNANAN 961 NNGTCTGANN NCNGCTNCNN CTNNNNNGNN NNCNNNNNNN NNN //
Primer: CMV_Forward (Pr00016) Region: CMV pro, EGFP 5' Sequence file: RDB17232_A9Eld.seq
>D06280B5_A9El_1_CMV_Forward_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC 121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC 361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG 601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC 661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC 721 ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG 781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGNCGANC TGTACAAGTC CGGANTCAGA 841 TCCGACCCCN ATGCCNAGGN CATCCGAGAN CTTCNAGAGG AAGTGGAGAA ANNNANGGAN 901 CAGCTCTCGC ANGNNNANGC CNTGNANNNN CCCCNNNNCN NAAGGANANN CNCGAANANN 961 NTGNNNANNC TGANAAANGN ANCTNNNNGN NNNNNNNGGN ANNANNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB17232_A9Ele.seq
>D06280B5_A9El_1_SV40pro_ori_F_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG CCNCNGCCTC TGNNCTATTC NNGAAGTAGT GAGGANGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA 121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG 181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG 241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC 301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC 361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG 421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC 481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA 541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG 601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG 661 GCGANGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG 721 GCCGCTTTTC TGGATTCATC GACNGNGGCC GGCTGGGNGT GGCGGANCGC TATCANGACA 781 TAGCGTTGGC TACCCGNNAT ATNGCTGNNN NNCTNGGCNG CNAANGGGNN GANNGCTNNC 841 TCNNNCTTNA NNGNATCGCC GCNCCNNATT CNCNNCNNNN CGCNNTNNNN NCNNCNNNNN 901 NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496.
reference	Ishida, M., Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Sci. Rep. 5: 8238 (2015). PMID 25649263.

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl