Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-bsr-Myc-KIF13A

Retroviral expression vector of mouse KIF13A fused with Myc.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)
Additional terms and conditions for distribution	Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。
付加的提供条件	pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17234_B0Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F Region: pol region, Myc, insert 5' Sequence file: RDB17234_B0Fma.seq
>D06280B7_B0Fm_3_MMLV-pol_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNGGCN TCGCANCTTN GNNACACGCC GCCCACGTGA AGGCTGCCGA    61 CCCCGGGGGT GGNCCATCCT CTAGACTGCC GGATCAGCTT GGCATGGAGC AAAAGCTCAT   121 TTCTGAAGAG GACTTGACGC GTGATTATGA TATTCCAACT ACTGCTAGCG AGAATTTGTA   181 TTTTCAGGGT GAGCTCGGAT CCATGTCGGA TACGAAGGTA AAAGTTGCCG TCCGGGTCCG   241 GCCCATGAAC CGAAGAGAGC TGGAACTGAA CACCAAGTGC GTGGTGGAGA TGGAAGGGAA   301 CCAGACGGTC CTGCACCCTC CTCCTTCCAA CACTAAGCAG GGAGAAAGAA AACCTCCCAA   361 GGTGTTTGCC TTTGATTATT GCTTTTGGTC CATGGATGAA TCGAACACAA CAAAATATGC   421 TGGTCAAGAA GTCGTTTTCA AGTGCCTCGG GGAAGGAATT CTCGAGAAAG CCTTTCAGGG   481 ATACAATGCT TGTATTTTTG CATATGGCCA GACAGGTTCT GGAAAGTCCT TTTCCATGAT   541 GGGCCACGCA GAGCAGCTGG GCCTTATCCC AAGGCTCTGC TGTGCTCTGT TTCAAAGGAT   601 CGCCCTGGAG CAAAATGAGT CACAGACCTT CAAAGTCGAG GTATCGTATA TGGAGATCTA   661 TAACGAGAAG GTCCGGGACC TCTTGGANCC CAAAGGGAGC ANGCAGTCTC TGAAAGTCCG   721 ANAGCATAAN GTCCTGGGGG CCGTATGTCG ATGGTCTGTC TCAGCTGGCT GTCACCAGCT   781 TTGAGGANAT TGNNTCACTG ATGTCTGANG GAAATAAGTC TCGAACGGNA NCAGCGNNCA   841 ACATGANTGA NGANAGCAGC CGTTCCCATG CNNNCTTCAA CATCNTCATC ACACAGANNC   901 NGNANNNNCN ACANNNNGGG NANNTNCNNG GGNNNNANNN TCANCNNNGN NNNNNCNNNG 961 NNNNNNNNNN //
Primer: IRES-R Region: insert 3' Sequence file: RDB17234_B0Fmb.seq
>D06280B7_B0Fm_3_IRES-R_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGN CNNNNNNNNN NGGGGGGGGG GGCGGNATTG ANCCCGCTAG    61 AGGGAAACCG TTGCTNNNNN NNNNTAGTTA ACAATTGTTA CCGCGGCCGC GGGAATTCGA   121 TTTTACCGAG TGGCTGTGGC TTCCTGGTCG TTCTCTATCT TGGGATGCAG CCGGTCGGCA   181 TCTGGAGGTC TTTCTGGGAT ACCTTTATGG TCTGTTTCGT TTGTTAGAGC CCTCCTCCTG   241 GAGGGCTCCC CTACAGAACA ATCTGCCAAA CCATCAAAGT CTTTGCCATC AGAAACACCC   301 ATGAACTCTG TGAAGGAATG GTCCTCCAAA ATGTTTGTAG TGTGTTCTAT CGTCACCTCT   361 CCTGGGCAGA ATTCTCTGGC TTGGTGGCCA TCTTTGCTGG GGCCGACATC CAGTTCTGAA   421 CATGAAGCAG TCCTGCATGG CATTCTGGAA CTTTCCTCAG GGATGCTCTG GGGTGACGGG   481 GTCCCCTTGG GTAAGGCACT GTTTTCCTCC AGCGGCACAG CGATTGTCTC ATCCTTCCCT   541 GAGCCTCTTC CTACTTCTGT AAGCTCCTGA TTCGAAGCTG GTCTGACATC CGGTGCAAGT   601 GATGGTCCCG GGGGCTCGGC GCACTCGACC TCCTTTGTCG AGACGGCAAG CTGGTCTGAG   661 CTGTCNCTGG ANGGGACTGC CATGTCAGAC AGAGTGGCAT TGGAGGCNCT GTGGGANAAG   721 TAGCCACTGG TGAGGCTGCT GGTGGTAGGG CTACGGGACA CTGCTTTCTC CAAGGCTCTC   781 GANNCTGTCA GGTCCGCCTT ANAAGANGNN CACTCCNNNT TCTCCAAGCT GGCATTGTAA   841 ACNCTNAANT CNGCNNNNNN CNNNGGGCAN NNNANGNTGT GNANGCATCA GNNNNCNTNN 901 TNANNNCNNN CNNNNNCNTT TTNNNNCNNN NNNNNNN //
Primer: MMLV psi_F Region: MMLV psi, gag Sequence file: RDB17234_B0Fmc.seq
>D06280B7_B0Fm_3_MMLVpsi_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNCNNNNNN NTGNNNTTTT GCTTTCGGTT TGGGNNCGAA GCCGCGCCGC    61 GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT   121 GTCTGAAAAT ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA CCTTAGGTCA   181 CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA GATGTCAAGA AGAGACGTTG   241 GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC GGATGGCCGC GAGACGGCAC   301 CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG   361 ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC   421 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT CCGCCCCGTC   481 TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC TCCCTTTATC CAGCCCTCAC   541 TCCTTCTCTA GGCGCCCCCA TATGGCCATA TGAGATCTTA TATGGGGCAC CCCCGCCCCT   601 TGTAAACTTC CCTGACCCTG ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA   661 CTTACAGGCT CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CAGCCTANCA   721 AGANCNACTG GANCGACCGG TGGTACCTCA CCCTTACCGA GTCGGCGACA CAGTGTGGGT   781 CCGCCGANAC NAGACTAAGA NNCTANAACC TCGCTGGAAN NGANCTTACA CAGNNCNGCT   841 GNNCACCCCC NACNGNCCNT CAANNNANAC NGNATCNCAN NTNGGNATAC NCGCCNNCNA   901 CNNNNAANGG NNNNCNGAAN CCCCNGGGGG NNGNNNNNNC CNNNNNNACN NNCNNGNNNN 961 ANNNNTNNNN NNNNNN //
Primer: Amp_R Region: Amp pro, CMV en, delta U3 5'LTR Sequence file: RDB17234_B0Fmd.seq
>D06280B7_B0Fm_3_Amp_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNGGGTTA TTGTCTCNNT GNGCGGANAC    61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA   121 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT   181 ATCACGAGGC CCTTTCGTCT TCACTCGAGG AGCTTGGCCC ATTGCATACG TTGTATCCAT   241 ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT   301 TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG   361 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC   421 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT   481 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC   541 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG   601 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG   661 CTATTACCAT GCTAGAGAAC CATCAGATGT TTCCNGGGTG CCCCAAGGAC CTGAAANTGA   721 CCCTGTGCCT TATTTGAACN NNCCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT   781 GCTCCCCGAG CTCAATAAAA GANNCNACAA CCCCTCACTC GGCGCGCCAG TCCTCCGATT   841 GACTGANTCG CCCGGNTANC CNNGTATCCA ATAAANCCCT CNNNNANTTG CATCCNANNT   901 NNNGGTCNNN CNNNTNCCNT NNGNANGNTC TCCNCTGANN NNNTGANNNN CCNNNNNCNN 961 NNNNTTNNNN TNNNNGGNNT NNNCNNNNNN NNNN //
Primer: IRES-F Region: IRES, bsr, 3' LTR Sequence file: RDB17234_B0Fme.seq
>D06280B7_B0Fm_3_IRES-F_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCGNNGT TAANAAAACG TCTAGGCCCC CCGAACCACG    61 GGGNNGTGGT TTTCCTTTGN AAAACACGAT AATACCATGG TCATGAAAAC ATTTAACATT   121 TCTCAACAAG ATCTAGAATT AGTAGAAGTA GCGACAGAGA AGATTACAAT GCTTTATGAG   181 GATAATAAAC ATCATGTGGG AGCGGCAATT CGTACGAAAA CAGGAGAAAT CATTTCGGCA   241 GTACATATTG AAGCGTATAT AGGACGAGTA ACTGTTTGTG CAGAAGCCAT TGCGATTGGT   301 AGTGCAGTTT CGAATGGACA AAAGGATTTT GACACGATTG TAGCTGTTAG ACACCCTTAT   361 TCTGACGAAG TAGATAGAAG TATTCGAGTG GTAAGTCCTT GTGGTATGTG TAGGGAGTTG   421 ATTTCAGACT ATGCACCAGA TTGTTTTGTG TTAATAGAAA TGAATGGCAA GTTAGTCAAA   481 ACTACGATTG AAGAACTCAT TCCACTCAAA TATACCCGAA ATTAAAAGTC GACGATAAAA   541 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT   601 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA   661 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA   721 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NNGCCAAGAA CAGATGGAAC AGCTGANTAT   781 GGGCNNAACN NGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCNNGGCC NNGAACAGAT   841 GGTCCCCANA TGCGGNCNAG CCCTCAGCAN TTNCTANANA NNCATCANAT GNTTTCCNNG   901 GTGNCCCNAN GGANCNNAAN NGANCCCTNN NNCNTNATTT NNANNNNANN NNANTCANNN 961 CGCNTNNNNN NNTNNNNNNN NNGNNNNTNN NNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl