Retroviral expression vector of mouse KIF13B fused with HA.
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External Database mouse Kif13b Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977) |
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Additional terms and conditions for distribution | Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested. |
Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() |
Remarks | Remember that you will be working with samples containing infectious virus. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Etoh, K. and Fukuda, M. (2019) J. Cell Sci. 132, jcs226977)。 |
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付加的提供条件 | pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。 |
提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17235 | pMRX-puro-HA-KIF13B | DNA solution |
Electronic file
Original reference
original | Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. |
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Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17235_B0Fmp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: MMLV-pol_F Region: pol, HA, insert 5' Sequence file: RDB17235_B0Fma.seq ![]() |
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>D6280B8_B0Fm_2_MMLV-pro_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNGGN ATCGCANCTT GGNNACNCGC CGCCCNCGTG AAGGCTGCCG 61 ACCCCGGGGG TGGANNNTCC TCTAGACTGC CGGATCTCCA CCATGGGTTA CCCATACGAC 121 GTCCCAGACT ACGCTGGATC CATGGGAGAC TCCAAAGTGA AAGTGGCGGT ACGGGTCCGG 181 CCAATGAACC GACGAGAAAT TGACTTACAT ACCAAATGTG TGGTGGATGT TGAAGCGAAC 241 AAAGTTATTC TGAATCCTGT AAATACAAAT CTTTCCAAAG GAGATGCCCG GGGCCAGCCA 301 AAGATCTTTG CTTATGATCA CTGTTTCTGG TCCATGGATG AATCTGTCCG AGAGAAGTAT 361 GCAGGTCAAG AAGATGTTTT CAAGTGCCTT GGGGAGAACA TTCTTCAGAA TGCATTTGAT 421 GGCTACAACG CCTGTATCTT TGCTTATGGG CAGACAGGGT CTGGAAAGTC TTACACCATG 481 ATGGGCACTG CTGACCAACC TGGATTAATC CCAAGGCTCT GTAGTGGACT CTTTGAACGA 541 ACCCAGAAAG AGGAAAATGA AGAACAGAGT TTTAAAGTCG AAGTGTCGTA CATGGAAATT 601 TATAATGAAA AAGTCCGAGA CCTTCTTGAC CCCAAAGGAA GCCGTCAGAC GTTGAAAGTG 661 AGAGAGCACA GTGTGTTGGG ACCCTACGTG GATGGACTTT CTAAACTGGC TGTCACAAGC 721 TACAAGGATA TTGAGTCTCT GATGTCTGAN GGCAACAAGT CTCGGACAGT TGCTGCAACC 781 NACATGAACG ANGAAAGTAG CCGTTCNNNT GCAGNCTTCN AAATCNCCNN GACNCATACT 841 CTGNATGATG TGAANTCTGG GANATCTGGN NAGAAAGTNG GCAANCNNAN CTTANNNNNN 901 TNNNCNNNNA NNGANCGANC NNNNAAANNN NNNNCTGCNN NNNANAGGNN NNNNNAGGNN 961 ANCAACNTNN NNNNNNNCNN NNNNNNNN // |
Primer: IRES-R Region: insert 3' Sequence file: RDB17235_B0Fmb.seq ![]() |
>D06280B8_B0Fm_2_IRES-R_E06_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAG GGGGGGGGGC GGNNNTTACG TAGCGGCCGC 61 GAATTCGATT TTAGCTGGCC CAGGACTTCC GGTTCTCAGG ATTCTTGTAA CTCCTGTCTC 121 CCTTGGCCAG TGCAGCTGTC AGTGAAGCCA GGTTGGTGGC AGAGCCCGAG ATGGTAGCAC 181 TTCTGCGGAC CTCTGGGGCT CCCTGAGGCT GGAGCCCTGA ACTGCGCCTG CGCCCAGTGC 241 CCACAGCCCT GCGGACCCGG CTGGGCCTCA CCAGCAGCCC ATAGCCAGGG TTGCACCTGA 301 AATACTGTTT CCCACCAATG GAGCCATCAT TCTTTCCTGC AGGCAGATCC AGCTCCACAC 361 CAATCCATGT TCCCTCCTGG AAATCAGTTG GTCCAATATA CCGTACAATG CCCGTCTTGT 421 TGGTGCCCAC AACAACATAC TCTCCCTCTC TGAGCCACTC AGGGACTTCG CTGGCATCCT 481 CAGAGTCAGA TGTTACTTCT AGCCTCCCCA GAGTCTGTCC CCTAGCATTG CTGGGGTCTT 541 CTGAAGCCAG GGGGTCCTCC TCTGCTCCGG ANGAGGCACC ACCCAGCATG CCTGTGAAGG 601 ACTTAAGCTC TGANGTCCGC ACTTTCCGGA TTCTGAATGG GGACGTGGGT ACAGCAGACG 661 CGGGTTCAGA AAGAANAGGT GCTCTGGGAA CCTCCAGCTC TGTGCTTTGA GTAGGAGCTG 721 CAGGTACGTG GGCCTCCTCT GGNGCANGAN GANGGCTTAN TGTGCAGGAN ANGAAAGCCA 781 GCTCANGCTC TGNGNTGACN TGNCNCANGG CANGGGGGGA NNNANGCGTC NGNCTGNNNA 841 NGNCNGNGGN GNNNAGGCCN NANGNGAGTG TTTCTGANAN GNNNGCAGAN NNNNNNNNN // |
Primer: MMLV psi_F Region: MMLV psi, gag, pol Sequence file: RDB17235_B0Fmc.seq ![]() |
>D6280B8_B0Fm_2_MMLV psi_F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNT GATTTTTGNT TTCGGTTTGG GANCGAAGCC GCGCCGCGCG 61 TCTTGTCTGC TGCAGCATCG TTCTGTGTTG TCTCTGTCTG ACTGTGTTTC TGTATTTGTC 121 TGAAAATATG GGCCCGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG 181 GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT 241 TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT 301 TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA 361 CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG 421 GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT 481 CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC 541 TTCTCTAGGC GCCCCCATAT GGCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT 601 AAACTTCCCT GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT 661 ACAGGCTCTC TACTTAGTCC AGCACGAAGT CTGGAGANCT CTGGCGGCAG CCTACCAAGA 721 ACAACTGGAC CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG 781 CCGACACCAG ACTAAGAACC TAGAACNTCG CTNGAAAGGA NCTTACACAG NCCTGCTGAN 841 CACCCCCACC GCCCTCNAAG TANACGGCAT CGCANCTNNN NNACNCGCCG CCCNCNNNAA 901 NGNNGNCNNN CCNNGGGGGN NGGANCNNNC NNNNNNNNGN CNNNNNNNCN NCNNNGNNNA 961 CCNNTACNNN NNNCNNNNNN NNNN // |
Primer: IRES-F Region: IRES, PuroR Sequence file: RDB17235_B0Fmd.seq ![]() |
>D06280B8_B0Fm_2_IRES-F_F06_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNCNNNGTTA ANANAACGTC TAGGCCCCCC GAACCACGGG 61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA CCGAGTACAA 121 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC 181 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG 241 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG 301 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG 361 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC 421 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT 481 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT 541 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC 601 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC 661 CGAAGGANCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA 721 CCCGCAGCGC CCGACCNNNN GANCGCACGA NCCCATGGCT CGACNATAAA ATAAAANANT 781 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTANGTT TGGCAAGCTA 841 GCTTAAGTAN CGCNATTTTG CANGGCNTNN NAAAAATACN TNANNTNNNA ANNNAGAANT 901 TCANATNNAG GNCNNNANCN NATGGNANNN NCNGNNNNTG GGCNAANCNN NNNANCNNNN 961 GNNNANNNNN NNNNCNNNNC CNNGNNNNNN NNNN // |
Primer: PuroR_F Region: PuroR, 3' LTR Sequence file: RDB17235_B0Fme.seq ![]() |
>D06280B8_B0Fm_2_PuroR_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG NGNNNGANGN NCGCGCACCT GGTGCATGAN CCGCAAGCCC 61 GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT 121 GGCTCGACGA TAAAATAAAA GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC 181 CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT 241 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT 301 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT 361 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA 421 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT 481 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC 541 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC 601 CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG 661 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG 721 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC ATGTATCNAA 781 ATTAATTTGG TTTTTTTTCT TAAGTATTTA CATTAAATGG CCATAGTTGC ATTAATGNAT 841 CGGNCAACGC GCGGGGGANA GGCGGTTTGC GTATTGGGCG CNCTNCCGCT TNCTCGCNCA 901 CTNANTCGCT GCGNNCGGNC NNNCNGNNGC NGNGANCNGN NATCANCNCA CNCNANGGNG 961 GTANTACNNN NATCNNNNNN ANNNNNNGNN NNNNNNNN // |
Primer: Amp_R Region: AmpR pro, CMV en, delU3 5' LTR Sequence file: RDB17235_B0Fmf.seq ![]() |
>D6280B8_B0Fm_2_Amp_R_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNTGN NGCANTNATN NGGGTTATTG TCTCNTGAGC GGATACATAT 61 TTGAATGTAT TTNNNNNAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 181 CGAGGCCCTT TCGTCTTCAC TCGAGGAGCT TGGCCCATTG CATACGTTGT ATCCATATCA 241 TAATATGTAC ATTTATATTG GCTCATGTCC AACATTACCG CCATGTTGAC ATTGATTATT 301 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT 361 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC 421 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG 481 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT 541 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 601 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT 661 TACCATGCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGANCTGA AATGACCCTG 721 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC 781 CCGANCTCAA TAAAAGAGCC CACAACCCCT CANTCNGCGC GCCAGTCCTC CGATTGACTG 841 ANTCGCCCGG NNACCCGNGT ATCCNNTAAA CCCTCTNGCA NTTNCATNCN ANTTNNGGNN 901 NNGCNNTTCC TTGGGNNGNN NNNCNNNNAN NGNNTGNNTA CCNGNCNNCG GGGGNNNTTT 961 CNTTTGGGGG NNNCNNNNNG NNNNNGNNNN NCCNNNNCNN NGGNNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Etoh, K., Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. J. Cell Sci. 132 (5). pii: jcs226977 (2019). PMID 30700496. |
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2022.05.17
GNP_filter3_RDBDEP_html_220516.pl