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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab8B (#RDB17254)

Expression vector of mouse Rab8B fused with EGFP.

Clone info. Expression vector of mouse Rab8B fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab8b cDNA
Reference of insert sequence AB232601 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Matsui, T. et al. (2011) Traffic 12, 1432-1443)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17254 pEGFP-C1-Rab8B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-Rab8B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17254).

Reference section:

Matsui, T., Itoh, T., Fukuda, M., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17254_A9Elp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert
Sequence file: RDB17254_A9Ela.seq check
>D06280D9_A9El_2_EGFP-C2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTGGTC CTGCTGGANT TCGTGNCCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGAA GACGTACGAT
  121 TATCTGTTCA AGCTGCTGCT CATCGGCGAC TCCGGCGTTG GCAAGACCTG CCTCCTGTTC
  181 CGCTTCTCAG AGGACGCCTT CAACACCACC TTTATCTCCA CCATCGGAAT TGATTTTAAA
  241 ATCAGAACGA TAGAACTCGA CGGAAAGAAA ATTAAACTTC AGATATGGGA CACGGCGGGC
  301 CAGGAAAGAT TCCGAACAAT TACGACAGCA TACTATAGAG GAGCCATGGG AATTATGCTG
  361 GTCTATGACA TCACCAATGA AAAATCCTTT GACAATATTA AAAATTGGAT AAGAAACATT
  421 GAAGAGCACG CCTCTTCAGA TGTTGAAAGA ATGATCCTGG GTAACAAATG TGATATGAAC
  481 GACAAAAGAC AAGTGTCGAA AGAGAGAGGG GAGAAGTTAG CAATTGACTA TGGGATTAAA
  541 TTTTTGGAGA CAAGTGCAAA GTCGAGTACA AATGTAGAAG AGGCATTTTT TACACTTGCA
  601 CGGGATATAA TGACAAAACT CAACAGGAAA ATGAACGACA GCAATTCATC AGGAGCAGGT
  661 GGGCCAGTGA AGATAACAGA GAGCCGGTCT AAGAAGACCA GTTTCTTCCG CTGTTCTCTG
  721 CTTTGAATCA CTAGTGAATT CTGCAGTCGA CGGTACCGCG GGCCCGGGAT CCACCGGATC
  781 TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
  841 CCTCCCACAC CTCCCCCNGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
  901 GTTTATTGCA NCTTATAANG GNTACAAATA AAGCAATAGC ATCACAAATT TCACNAATAA
  961 ANCNNTTTTT TTCACTGCAT TCNAGTTNNN GNTTTGNNCN ANCTCNNCNA NGTATCNTNN
1021 ANNCNNNNNN NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB17254_A9Elb.seq check
>D06280D9_A9El_2_SV40polyA-R2_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA ANNNGTTAAC NACNANNATT GCATTCNTTT TATGTTTCAG
   61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  181 AGAATTCACT AGTGATTCAA AGCAGAGAAC AGCGGAAGAA ACTGGTCTTC TTAGACCGGC
  241 TCTCTGTTAT CTTCACTGGC CCACCTGCTC CTGATGAATT GCTGTCGTTC ATTTTCCTGT
  301 TGAGTTTTGT CATTATATCC CGTGCAAGTG TAAAAAATGC CTCTTCTACA TTTGTACTCG
  361 ACTTTGCACT TGTCTCCAAA AATTTAATCC CATAGTCAAT TGCTAACTTC TCCCCTCTCT
  421 CTTTCGACAC TTGTCTTTTG TCGTTCATAT CACATTTGTT ACCCAGGATC ATTCTTTCAA
  481 CATCTGAAGA GGCGTGCTCT TCAATGTTTC TTATCCAATT TTTAATATTG TCAAAGGATT
  541 TTTCATTGGT GATGTCATAG ACCAGCATAA TTCCCATGGC TCCTCTATAG TATGCTGTCG
  601 TAATTGTTCG GAATCTTTCC TGGCCCGCCG TGTCCCATAT CTGAAGTTTA ATTTTCTTTC
  661 CGTCGAGTTC TATCGTTCTG ATTTTAAAAT CAATTCCGAT GGTGGAGATA AAGGTGGTGT
  721 TGAAGGCGTC CTCTGAGAAG CGGAACAGGA NGCAGGTCTT GCCNACGCCG GAGTCGCCGA
  781 TGAGCAGCAN CTTGAACAGA TAATCGTACG TCTTCNCCAT GGATCTGANT NCGGACTTGT
  841 ACAGCTCGTC CATGCCNAGA GTGATNCCGG CNGNNGTCAN NANCTCCAGC NNNNCATGTG
  901 ATCGCNCTTC NCGTTGGGGT CTTTGCTCNN GNCGGANNGG GTGCTCNNGT ANTGGNTNGT
  961 NGGGNNNNNN CACNGGGNCN NNCGCNNANN GGGNGNNCTG CTNGNANNNG NNNNNNNANC
1021 NNCNNNCTNN NNNNNNNGAN NNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB17254_A9Elc.seq check
>D06280D9_A9El_2_CMV-Forward_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA
  121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG
  181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG
  241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC
  301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC
  361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT
  421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG
  481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA
  541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG
  601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG
  661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT
  721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ANCCCAACGA GAAGCGCGAT CACATGGTCC
  781 TGCTGGANTT CGTGANCGCC GCCNGGNTCA CTCTCGGCAT GGACGANCTG TACAAGTCCN
  841 GANTCANATC CATGGCGAAN ANGTACGANT ATCTGTTCAA GCTGCTGCTC ATNGGCGANT
  901 CCGGCGTTGG NAAGANCNGC CNNCNGNNCC GCTTNNCNNA NNNNGCCTNC NANNCCACCT
  961 TTATCTCCAC CNTCNGGAAT TGATTTTNAA ANCNGNNNNA TANNANNTCN NNNNNAANAA
1021 AANTNNANNN NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB17254_A9Eld.seq check
>D06280D9_A9El_2_SV40pro_F_V2_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT TNCNAAGNNC GATNNAGANN ACNGGNTGAG
   61 GNTCGTTTCN NNTGATTGAA CAAGNATGGA TTGCNCNCAG GTTCTCCGGC CGCTTGGGTG
  121 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
  181 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
  241 CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
  301 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
  361 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
  421 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
  481 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
  541 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
  601 AGCATGCCCG ACGGCGAGGA TNTNNNCGTG ACCNNNNNCG ATGCCTGCTT GCCGAATATC
  661 ATGGTGGAAA ATNGGCCGCT TTTCTGGATT CATNNNNTGT GGCCGGCTGG GTGTGGCGGA
  721 NCNNCTATCA GGACATAGCG TTNGCTACCC GTGATANTGC TGAANANCTT GGCNGCGAAN
  781 TGGGCTGACC GCTTNCTCGN GCTTTACNGT ATCNNCGCTC CNNATTCNCN NNGCATCNCC
  841 TTCTATCNCC TTCTTGACGA NTTCTTNNGN NCNGGNNNTC NNGGGGNNCN AANNNNNCNG
  901 NNCAAGCNAN NNCCNANCNN NNNANCNCNN ANATTNNNAT TNNNNCGCCG CCTTCTANNA
961 AANGGTNNGG GCNTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsui, T., Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic 12 (10): 1432-1443 (2011). PMID 21718402. [link to RRC of NBRP]
reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]