Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-Rab32

Expression vector of mouse Rab32 fused with EGFP.

Catalog number RDB17277
Resource name pEGFP-C1-Rab32
Clone info. Expression vector of mouse Rab32 fused with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name mouse Rab32 cDNA
Reference of insert sequence AB232627 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank

External Database
mouse Rab32

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          MTA, for use for not-for-profit academic purpose [Word]
          Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuda, M. (2003) J. Biol. Chem. 278, 15373-15380); (Tsuboi, T. and Fukuda, M. (2006) J. Cell Sci. 119, 2196-2203)。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17277 pEGFP-C1-Rab32 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEGFP-C1-Rab32 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17277).

          Reference section:

          Tsuboi, T., Fukuda, M., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17277_A9Elp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3',Insert 5'
          Sequence file: RDB17277_A9Ela.seq check
          >D06280G5_A9El_1_EGFP-C2_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN TCNNNTGGTC CTGCTGGANT TCGTGACCGC CGCCGGGNTC
             61 ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CCATGGCGGG CGAGGGACTA
            121 GGGCAACAGG GCGCGTCGGC CACCGCGGCG CCGGAGACCC GAGAGCACCT CTTTAAGGTG
            181 CTGGTCATCG GCGAGCTCGG CGTGGGTAAG ACGAGCATCA TCAAGCGCTA CGTGCACCAG
            241 CTCTTCTCCC AGCACTACCG CGCCACCATT GGCGTGGACT TCGCCCTCAA AGTTCTCAAC
            301 TGGGACAGCA GGACGCTCGT GCGCCTGCAG CTGTGGGATA TCGCGGGACA GGAACGGTTT
            361 GGCAACATGA CTCGAGTATA CTATAAGGAA GCTCTTGGAG CATTTGTAGT CTTTGATATA
            421 TCGAGAAGTT CTACATTTGA TGCAGTCCTA AAATGGAAAA ACGATCTGGA TAGTAAAGTT
            481 CATCTTCCCA ACGGCAGCCC CATTCCTGCT GTCCTCCTAG CTAACAAATG TGACCAGAAG
            541 AAGGACAACA GCCAGAGTCC TTCCCAGATG GACCAGTTTT GTAAAGACCA TGGCTTCACT
            601 GGATGGTTTG AAACCTCTGC CAAGGATAAT ATAAACATCG ACGAGGCCAC CCGCTTCCTA
            661 GTGGAAAACA TGCTTGCAAA CCAGCAAAGT TTTCCTAGTG AAGAAATCGA CCTGGACAGA
            721 ATTAAACTGG TAGAGGAGCC CCCCACAACA AAGCCCAGGT CCCAGTGCTG CTGANGTGTC
            781 TCTGTGAATC ACTAGTGAAT TCTGCAGTCG ANGGTACCGC GGGCCCGGGA TCCACCGGAT
            841 CTAGATAACT GATCATAATC AGCCNTACNA CATTTGNAGA NGNTTNACTT GCTTTAAAAA
            901 ANCTCCCNCA NCNCCCCCTG AANCNGAANN ATAAANNGAN TGNNATTGNN GNNGNNAACT
            961 TGNTTANNGN NGCTNANNAT GGNNNNNAAN NAANCNNNAN CNTCNCAANT TTCNNNAANN
          1021 ANNNNNTTTT TTNNNNNNNN NN
          //
          Primer: SV40polyA-R (Pr0109)
          Region: insert 3'
          Sequence file: RDB17277_A9Elb.seq check
          >D06280G5_A9El_1_SV40polyA-R2_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNGNNNNNAA NNNGTTAANN ANNACNATTG CATTCNTTTT ATGTTTCNGG
             61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
            181 GAATTCACTA GTGATTCACA GAGACACATC AGCAGCACTG GGACCTGGGC TTTGTTGTGG
            241 GGGGCTCCTC TACCAGTTTA ATTCTGTCCA GGTCGATTTC TTCACTAGGA AAACTTTGCT
            301 GGTTTGCAAG CATGTTTTCC ACTAGGAAGC GGGTGGCCTC GTCGATGTTT ATATTATCCT
            361 TGGCAGAGGT TTCAAACCAT CCAGTGAAGC CATGGTCTTT ACAAAACTGG TCCATCTGGG
            421 AAGGACTCTG GCTGTTGTCC TTCTTCTGGT CACATTTGTT AGCTAGGAGG ACAGCAGGAA
            481 TGGGGCTGCC GTTGGGAAGA TGAACTTTAC TATCCAGATC GTTTTTCCAT TTTAGGACTG
            541 CATCAAATGT AGAACTTCTC GATATATCAA AGACTACAAA TGCTCCAAGA GCTTCCTTAT
            601 AGTATACTCG AGTCATGTTG CCAAACCGTT CCTGTCCCGC GATATCCCAC AGCTGCAGGC
            661 GCACGAGCGT CCTGCTGTCC CAGTTGAGAA CTTTGAGGGC GAAGTCCACG CCAATGGTGG
            721 CGCGGTAGTG CTGGGAGAAG AGCTGGTGCA CGTAGCGCTT GATGATGCTC GTCTTACCCA
            781 CGCCGANCTC GCCGATGANC AGCACCTTAA AGANGTGCTC TCGGGTCTCC GGCGCCGCGG
            841 NGGCCGACGC GCCCTGTTGC CCTAGNCCCN CGCCCGCCAN GGATCNNANN NCNGANTTGT
            901 ACNGCTCGNC CNTGCCNANA NTGATNCCGG CGGNNNNCAC GAACTCCAGC ANGANCNTGN
          961 NNTCGCGCTT CNCNNTNNGG GNNTTTGNTC NNNNNGNNNN NGNNGNNCNN NNNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: EGFP 5'
          Sequence file: RDB17277_A9Elc.seq check
          >D06280G5_A9El_1_CMV_Forward_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNGC NNTGGGCGGT AGGCGTNNTA CGGTGNNNNN
             61 NTCTATATNA GCAGAGCTGG NNNNNNGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA
            121 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
            181 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
            241 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
            301 CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA
            361 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT
            421 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
            481 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
            541 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA
            601 ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG
            661 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACANCC
            721 ACTACCTGAG CACCCNGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG
            781 NCCTGCTGGA NTTCGTGACC GCCGCCGGGA TCNCTCTCGG CATGGACNAG CTGTACAAGT
            841 CCGGANTCAG ATNCATGGCG GGCGANGGAC TNNNGGCNAC NGGGCGCGNC NGNCCACCGC
            901 GGCGCCNGNN ANCCNANNNC NNCTCTTTAA NGNGCTGGNC ATCNGNCGAN NTCGGCGTGG
            961 GTAAGANNAN NATCATCNNG CGNTACGTGN NCCAGCNNNT NNTNCNAGCA CTNNNNNCNN
          1021 NNNCNNNNNN NNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB17277_A9Eld.seq check
          >D06280G5_A9El_1_SV40pro_F_V2_D01_10_ABI24.ab1
              1 NNNNNNNNNT TNNNGNNGNN NNNGCTTTTG CAAGNNCGAT CAAGAGACAG GATGAGGNTC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GANCGGGNNT CTGGGGTTCG AAATGANNGA NNNAGCGNCG
            901 CCCAACCNGN CATCACGAGN NTTCGANNNC NCCGCCNCCT NCTATGAAAG GNTGGGCTTC
          961 NNAATCGTTT NCCNNNNNNN NNGNNNGNNT GANNCNTNNN NCGNNGGNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [link to RRC of NBRP]
          reference Fukuda, M., Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2. J. Biol. Chem. 278 (17): 15373-15380 (2003). PMID 12578829. [link to RRC of NBRP]

          2024.03.26

          HTML_filter_RDB_240326.pl