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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET47-eIF4A1-19-406

Bacterial expression vecter of mouse eIF4A1 fragment from Pro19 to C-terminal Ile406.

Catalog number RDB17294
Resource name pET47-eIF4A1-19-406
Clone info. Bacterial expression vecter of mouse eukaryotic translation initiation factor 4A1 (eIF4A1) fragment from Pro19 to C-terminal Ile406 tagged with 6xHis at N-terminus.
Comment Expression was confirmed by the depositor with CBB staining. This clone contains a cDNA fragment corresponding to the 177nt to 1343nt of NM_144958.4. Known differences (at least) : G to C substitution at 179nt (P, synonym) ; C to T substitution at 1325nt (N, synonym) ; T to C substitution at 1340nt (I, synonym).
Vector backbone pET47 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Kan^r.
Gene/insert name Mouse Eif4a1 cDNA
Depositor|Developer Iwasaki, Shintaro |
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External Database
Mouse Eif4a1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。
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          RDB17294 pET47-eIF4A1-19-406 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pET47-eIF4A1-19-406 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17294).

          Reference section:

          Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K., Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17294_A9Dbp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBR_BamHI_R2 (Pr0141)
          Region: T7 pro,lacO,RBS,6xHis,HRV 3C site,insert 5'
          Sequence file: RDB17294_A9Dba.seq check
          >D06253A1_A9Db_4_pBR_BamHI_R2_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNCGNNNGGC NNANNNGNNC GAGATCGATC
             61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
            121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCAC ATCACCACCA
            181 CCATCACTCC GCGGCTCTTG AAGTCCTCTT TCAGGGACCC GGGTACCAGG ATCCCGAAGG
            241 CGTCATCGAG AGTAACTGGA ACGAGATTGT GGATAGCTTT GATGACATGA ATCTCTCAGA
            301 GTCCCTCCTC CGTGGTATTT ATGCCTATGG TTTTGAGAAG CCCTCTGCCA TCCAGCAGCG
            361 AGCTATTCTT CCTTGTATCA AGGGTTATGA TGTGATTGCT CAAGCCCAGT CTGGGACTGG
            421 GAAAACAGCT ACATTTGCCA TATCAATTCT GCAGCAGATT GAATTAGATC TAAAGGCCAC
            481 TCAGGCTTTG GTTCTGGCAC CCACACGTGA ATTGGCTCAG CAGATACAAA AGGTGGTTAT
            541 GGCATTAGGA GACTACATGG GTGCCTCTTG TCATGCCTGC ATTGGGGGCA CCAATGTGCG
            601 TGCTGAGGTG CAGAAGCTGC AGATGGAAGC TCCCCATATC ATCGTGGGTA CCCCTGGCCG
            661 GGTGTTTGAC ATGCTTAACC GGAGNTACCT GTCCCCCAAA TACATCAAGA TGTTCGTACT
            721 GGATGAAGCA NANGAAATGT TAAGCCNANG GTTCNAGGAT CANATCTATG ANATATTCCA
            781 NAAGCTCAAC NGCAACACAC AGGTAGTTTT GTTGTCTGCT ACNATGCCTT CTGATGTNCT
            841 TGANGTGANN NANAANTTTA TGANAGANCC TANTNNGNNT CNTGTNAAGA ANGGAANANN
            901 TGANCCNNGN ANGGNANNCN CCNNTTCTNC NTNNATGTGN AANGAGANGA NTGGNAANCT
          961 TGNNNNNNTN NTGNGANNNG NATGANNNNC TNANNATNNN CCAGNNNNNN NNNN
          //
          Primer: f1ori_R3 (Pr0142)
          Region: T7 ter,insert 3'
          Sequence file: RDB17294_A9Dbb.seq check
          >D06253A1_A9Db_4_f1ori_R3_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCCTTNNNG CNNNANCCCT CAAGANCCGT TTAGAGGCCC
             61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
            121 CTTTGTTTAG CAGCCTAGGT TAATTAAGCC TCGAGAGCAG CAGAAGTAGA GCTGTCCATG
            181 TGCTGGCGTT CGAATTTAGC AGCAGCGGTT TCTTTACTAC CGCGTGGCAC CAGAGCGAGC
            241 TCTGCGGCCG CAAGCTTGTC GACTCAGATG AGGTCAGCAA CATTGAGGGG CATCTCTTCA
            301 ATGGAGGTGT TGTAGAAAGT CTCAATGTCT CGAAGAGTCC TCTTGTCTTC TTCGGTCACC
            361 ATGTTAATAG CCACACCCTT ACGACCAAAC CGACCACCTC GACCGATTCT GTGGATGTAG
            421 TTTTCCCTGT TGGTGGGAAG GTCATAGTTG ATGACTAAGG AGACCTGCTG CACATCAATG
            481 CCTCTGGCCA ACAGGTCAGT GGTAATTAAT ACTCTGCTAG AGCCAGACCG GAACTCCCTC
            541 ATGATCACAT CTCGTTCCTT TTGGTCCATA TCTCCGTGCA TGGCAGAAAC AGTGAAATCT
            601 CGGGCATGCA TCTTCTCGGT GAGCCAGTCC ACCTTCCTTC TGGTGTTGAT AAAGATGACT
            661 GCCTGGGTGA TGGTCAGCGT CTCATACAAG TCACACAATG TGTCAAGCTT CCACTCCTCT
            721 CGTTCCACAT TGATGTAGAA TTGGCGGANA CCCTCCAGGG TCAATTCTTC CTTCTTGACA
            781 AGAATCCGAA TAGGGTCTCT CATAAATTTC TTGGTCACCT CAAGGACATC AGAANGCATT
            841 GTANCAGANA ACANAACTAC CTGTGTGTTG CTGTTGANCT TCTGGAATAT GTCATANATC
            901 TGATNCTTNA ANCCNCGGCT NAACNTTTCA TCTGCTNCAT CNNNNACGAA NNTCTNNNTG
            961 TATTTNGGGG GANAGGTATN NNCNNNNNNN NCANGNNAAA NNCCNNNNCN NNGGNNNNNC
          1021 NNNNNNNNNN
          //
          Primer: lacIpro_F (Pr0409)
          Region: lacI pro,lacI
          Sequence file: RDB17294_A9Dbc.seq check
          >D06253A1_A9Db_4_laclpro_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNATTC NGGGTGGTGA ATGTGAAACC
             61 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
            121 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
            181 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
            241 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
            301 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
            361 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
            421 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
            481 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
            541 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
            601 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
            661 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
            721 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAANNGAGGG CATCGTTCCC ACTGCGATGC
            781 TGGTTGCCAN CGATCAGATG GCGCTGGGCG CANTGCGCGC CATTACCNAG TCNNGGCTGC
            841 NCGTTGGTGC GGNNATCTCN NNNNNTGGGA TANNANNNAT ACCNAANACA GCTCNNGTTA
            901 TATCCCGCCG TTAACCACCN TCAAANNNGN NTTTCNNCNG CNNGGGGCAA ACNNNCNNGN
          961 ANCNNNTNGC TGNNNCTCTC TCNNGNCNNN NNNNNGANNG NNAANNANCN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl