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pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met)

Gateway based destinatin vector of human N-terminal region (NTD, 1-237) of eIF4A1 substitutions (F163L, I199M) tagged with 6xHis and MBP at N-terminus.

Catalog number RDB17305
Resource name pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met)
Clone info. Gateway based destinatin vector of human N-terminal region (NTD, 1-237) of eukaryotic translation initiation factor 4A1 (eIF4A1) substitutions (F163L, I199M) tagged with 6xHis and MBP at N-terminus.
Comment Expression was confirmed by the depositor with CBB staining.
Vector backbone pET-28a (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r.
Gene/insert name human EIF4A1 cDNA
Depositor|Developer Iwasaki, Shintaro |
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External Database
human EIF4A1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iwasaki S. et al. Mol. Cell. 73(4): 738-748. e9, 2019)。
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          RDB17305 pHISMBP-eIF4A1-NTD (Phe163Leu-Ile199Met) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Iwasaki, S., The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol. Cell 73 (4): 738-748.e9 (2019). PMID 30595437.

          2022.05.17

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