Resource data sheet
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pGL3-AID region 1 (positions positions -1500 to +101) (#RDB17354)

Luciferase reporter of the mouse Aicda promoter (positions -1500 to +101).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hanihara-Tatsuzawa, F., J. Biol. Chem. 289 (45): 30925-30936 (2014). PMID 25124037. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Luciferase reporter of the mouse activation-induced cytidine deaminase (Aicda) promoter (positions -1500 to +101). Four regions within the genomic Aicda locus are highly conserved among many species.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL3 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37℃
Gene/insert name mouse Aicda genomic
Depositor|Developer Maruyama, Takashi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanihara-Tatsuzawa, F. et al. J. Biol. Chem. 289(45) : 30925-30936, 2014) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Tatsushi Muta is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanihara-Tatsuzawa, F. et al. J. Biol. Chem. 289(45) : 30925-30936, 2014)。利用者は、研究成果の公表にあたって寄託者ならびに牟田達史博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB17354 pGL3-AID region 1 (positions positions -1500 to +101) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGL3-AID region 1 (positions positions -1500 to +101) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17354).

Reference section:

Hanihara-Tatsuzawa, F., Miura, H., Kobayashi, S., Isagawa, T., Okuma, A., Manabe, I., MaruYama, T., Control of Toll-like receptor-mediated T cell-independent type 1 antibody responses by the inducible nuclear protein IkappaB-zeta. J. Biol. Chem. 289 (45): 30925-30936 (2014). PMID 25124037. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hanihara-Tatsuzawa, F., Control of Toll-like receptor-mediated T cell-independent type 1 antibody responses by the inducible nuclear protein IkappaB-zeta. J. Biol. Chem. 289 (45): 30925-30936 (2014). PMID 25124037. [PubMed] [Article] [RRC of NBRP]