Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

ZBTB24 px330

Expression vector of guide RNA for targeting human ZBTB24 exon 4.

Catalog number RDB17379
Resource name ZBTB24 px330
Alternative name ZBTB24 exon4 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human zinc finger and BTB domain containing 24 (ZBTB24) exon 4 (5'-GTGATCAATGCGGAAAATAT-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human ZBTB24 dsOligo
Depositor|Developer Unoki, Motoko |
Other clones in our bank
External Database
human ZBTB24
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17379 ZBTB24 px330 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The ZBTB24 px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17379).

          Reference section:

          Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17379_A9Fhp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3'element reverse (Pr0108)
          Region: U6 pro, gRNA (ZBTB24) gRNA scaffold, CBh pro 5'
          Sequence file: RDB17379_A9Fha.seq check
          >D06399A6_A9Fh_2_SV40pA3'element reverse_H02_23_ABI24.ab1
              1 NNNNNNNNNN GNNGGNNNNN TGGNANNNGC NNGCAACGCG GCCTTTTTNC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTGAGG GCCTATTTCC CATGATTCCT TCATATTTGC
            121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA
            181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
            241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
            301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGATAT TTTCCGCATT GATCACGTTT
            361 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA
            421 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG
            481 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGGTACCCG TTACATAACT
            541 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAGT
            601 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
            661 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
            721 TAAATGGCCC GCCTGGCATT GTGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
            781 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAN GTGANCCCCA CGTTCTGCTT
            841 CACTCTCCCC ATCTCCCCCC CCCTCCCCAC CCCCNATTTT GTATTTATTT ATTTTTTAAT
          901 TATTTTGTGC AGCGANGGGG NNNNNNNNNN
          //
          Primer: Cas9-N_R1 (Pr0650)
          Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3'
          Sequence file: RDB17379_A9Fhb.seq check
          >D06399A6_A9Fh_2_Cas9-N_R1_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTNGTNN TCGTCGGTGN NCNCGGCCCN GCCCNCAGAG
             61 TTGGTGCCGA TGTCCAGGCC GATGCTGTAC TTCTTGTCGG CTGCTGGGAC TCCGTGGATA
            121 CCGACCTTCC GCTTCTTCTT TGGGGCCATC TTATCGTCAT CGTCTTTGTA ATCAATATCA
            181 TGATCCTTGT AGTCTCCGTC GTGGTCCTTA TAGTCCATGG TGGCACCGGT CCAACCTGAA
            241 AAAAAGTGAT TTCAGGCAGG TGCTCCAGGT AATTAAACAT TAATACCCCA CCAACCAACC
            301 ATCCCTTAAA CCCTTACCTC TTGCTCAGCT AATTACAGCC CGGAGGAGAA GGGCCGTCCC
          361 GCCCGCTCAC CTGTGGGAGT AACGCGGTCA GTCAGAGCCG GGGCGGGCGG CGCGAGGNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH p(A), nucleoplasmin NLS, Cas9 3'
          Sequence file: RDB17379_A9Fhc.seq check
          >D06399A6_A9Fh_2_BGH_rev2_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGNNGNNGG GCNAACNACA GATGGCTGGC AACTAGAAGG
             61 CACAGTCGAG GCTGATCAGC GAGCTCTAGG AATTCTTACT TTTTCTTTTT TGCCTGGCCG
            121 GCCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCCAGCT GAGACAGGTC GATCCGTGTC
            181 TCGTACAGGC CGGTGATGCT CTGGTGGATC AGGGTGGCGT CCAGCACCTC TTTGGTGCTG
            241 GTGTACCTCT TCCGGTCGAT GGTGGTGTCA AAGTACTTGA AGGCGGCAGG GGCTCCCAGA
            301 TTGGTCAGGG TAAACAGGTG GATGATATTC TCGGCCTGCT CTCTGATGGG CTTATCCCGG
            361 TGCTTGTTGT AGGCGGACAG CACTTTGTCC AGATTAGCGT CGGCCAGGAT CACTCTCTTG
            421 GAGAACTCGC TGATCTGCTC GATGATCTCG TCCAGGTAGT GCTTGTGCTG TTCCACAAAC
            481 AGCTGTTTCT GCTCATTATC CTCGGGGGAG CCCTTCAGCT TCTCATAGTG GCTGGCCAGG
            541 TACAGGAAGT TCACATATTT GGAGGGCAGG GCCAGTTCGT TTCCCTTCTG CAGTTCGCCG
            601 GCAGAGGCCA GCATTCTCTT CCGGCCGTTT TCCAGCTCGA ACAGGGAGTA CTTAGGCAGC
            661 TTGATGATCA GGTCCTTTTT CACTTCTTTG TAGCCCTTGG CTTCCAGAAA GTCGATGGGA
            721 TTCTTCTCGA AGCTGCTTCT TTCCATGATG GTGATCCCCA GCAGCTCTTT CNCACTCTTC
            781 AGTTTCTTGG ACTTGCCCTT TTCCACTTTG GCCACCACCA GCACAGAATA NGCCACGGNN
            841 NGGCTGTCGA AGCCGCCGTA CTTCTTANGG NCCCAGNNCT TCTTTCTGGC GATCAGCTTA
            901 TCGCNNTNCC TCTTGGGCAG GATAGANTCT TTGCTGAAGC CGNCTGNCTG CNNNNCGGNC
            961 TTTTTCNCNA NATNCACTNN GGGGCANGNN CAGCNNTTNN CNGNNCNGNN NNNNAANNNN
          1021 NNGNNNNNTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl