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pGBD-C1-Rab11A-delta Cys-S25N (#RDB17406)

Expression vector of constitutive negative mutants of mouse Rab11A (S25N) for yeast two-hybrid assay.

Clone info. Expression vector of constitutive negative mutants of mouse Rab11A (S25N) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin, TRP1
Gene/insert name mouse Rab11a cDNA
Reference of insert sequence AB232605 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kuroda, T. S. et al. J. Biol. Chem. 277, 9212-9218, 2002); (Tamura, K. et al. Mol. Biol, Cell 20, 2900-2908, 2009)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17406 pGBD-C1-Rab11A-delta Cys-S25N Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab11A-delta Cys-S25N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17406).

Reference section:

Tamura, K., Ohbayashi, N., Maruta, Y., Kanno, E., Itoh, T., Fukuda, M., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]
reference Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082. [link to RRC of NBRP]