Please review the QC test results indicated by icon below as well as clone information before placing your order.

pETDuet-eIF2Bd-eIF2Bb

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit beta (eIF2Bbeta) and human eukaryotic translation initiation factor 2B subunit delta (eIF2Bdelta).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17723_B0Adp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBE322_BamHI_R Region: lacO, RBS, insert1 5' Sequence file: RDB17723_B0Ada.seq
>D06721A2_B0Ad_2_pBR322_BamHI_R_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNNNNNNNN TCNCTATNNN NNNAANTGTG AGCGGANAAC    61 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG   121 CAGCCAGGAT CCGAATTCGA GCTCGGCGCG CCTGCAGGTC GACGCTGCTG TGGCCGTGGC   181 TGTTCGCGAG GACTCGGGAT CCGGGATGAA GGCGGAGCTT CCCCCCGGGC CTGGGGCAGT   241 GGGGAGGGAA ATGACCAAAG AAGAAAAGCT GCAGCTTCGG AAGGAAAAGA AACAGCAGAA   301 GAAGAAACGG AAGGAAGAAA AGGGGGCAGA ACCAGAGACT GGCTCTGCTG TATCTGCAGC   361 CCAATGTCAA GTAGGCCCAA CCAGAGAACT GCCAGAATCG GGCATTCAGT TGGGCACTCC   421 TCGGGAGAAA GTTCCAGCTG GTCGGAGTAA GGCCGAACTT CGGGCTGAGC GTCGAGCCAA   481 GCAGGAGGCC GAGCGGGCCC TGAAACAGGC AAGAAAAGGG GAACAAGGAG GACCACCTCC   541 TAAGGCCAGC CCCAGCACAG CTGGAGAAAC CCCCTCAGGA GTGAAGCGTC TCCCTGAGTA   601 CCCTCAGGTT GATGACCTAC TTCTGANAAG GCTTGTTAAA AAACCAGAGC GTCAACAGGT   661 TCCTACACGA AAGGATTATG GATCCAAAGT CAGTCTCTTC TCTCACCTAC CCCAGTACAG   721 CAGACAAAAC TCTCTGANCC AGTTTATGAG CATCCCATCC TCTGTGATCC ACCCAGCCNT   781 GGTGCGACTC GGCCTGCAGT ACTCCCAGGG CCTGGTCAGT GGCTCCAATG CCCGGNGTAN   841 TGNCCNGCTT NCGNGNCNNG CANCNNNGNN NTCNNGANTA NNCNACACCG NCNTAANGNA   901 AGAACTCNNC NGGGNTCTAG TGNANNNNTN AAANCCTNNN TGANNNNNNN NNANNCNNNN 961 NNNNNCCNNN NCNNNNNNNN NN //
Primer: hEIF2Bb_R Region: insert2 5', RBS, lacO, T7 pro, insert1 3' Sequence file: RDB17723_B0Adb.seq
>D06721A2_B0Ad_2_hEIF2Bb_R_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGCCCTCT CNGNNNNNNN    61 NNNNNNNNNG CTCCCCCGCG TTGNNNNNNN GGTGGTCCGT GATGATCTGG CGCAGCAACC   121 CTAGNNNNNN CCGAGCCATT TCCTCGGAGC TGCGCGGCCC ACCACCCCGC TTCAGGGTCT   181 CCACGAAGCT CTCGATCCTC TCTGACAACT CCGAGCCCTT CGCTGCGGAT CCCGGCATAT   241 GTATATCTCC TTCTTATACT TAACTAATAT ACTAAGATGG GGAATTGTTA TCCGCTCACA   301 ATTCCCCTAT AGTGAGTCGT ATTAATTTCG ATTATGCGGC CGTGTACAAT ACGATTACTT   361 TCTGTTCGAC TTAAGCATTA TGCGGCCGCT CACTGGTCAC TGCTCTTGAC TCGTAGAACA   421 ACAGGTACAG AACTGCAAGG GATCATCCCC AGCTCCGTGA TCACCAGATC CACAAGCTCT   481 GGGGGAGTCA CATCATAGAC TAGATTCAAC AACCGTAGGG ATGCGTGGTT CTGCCAGTTA   541 GCCAGCGCAA CATGTTCTCC CCGCTTACAT TGCAGATCAT CAGGGTCATC TAGCTCATTA   601 GAGACAAAGG CATCAGTCTG CACACGCTCA CAGAACTTGT ATGTTTNNNN NNAAACCANC   661 ACTGGTACAT TATGGGCTCG AGCCACCAGG GCTAACTGTG CTGTCCCTAC CCGTGACATC   721 ACAGACCCGT TGGCCAANAN TGCATGAGCT CCCAATAGCA CCTTGGAAAC CTCTGGGAGC   781 ANNTANGANG CTGCANGAAT CANCAGGTAG GANGNTGGGA CNNNAGCATG GACTANNNAN   841 NGTAGTGTGT GNCNTTCCTT CNNGCCATGG CCGGCTGTCC ANCANNNNCC NNCNGAANNC   901 GNCNGNCCTC TGNNNNNNNC TNNNNNNANA NNNNNNNGAT NNCNNNAGAT GAGCNTCNNN   961 NNTNCCNNNN ATCANNNNNN NCNNTTNNNN GANNNTNNTG GNNNANNCAA AAGNNNNGNA  1021 AANNNGNCNT GNANCNNNNN NNCNNCNANN NNNNCNNCNT NGNNNNNNNA NCNNNNNNNN 1081 NANNNGNNNN NNNNNN //
Primer: T7 terminator Region: insert2 3' Sequence file: RDB17723_B0Adc.seq
>D06721_A2_B0Ad_2_T7terminator_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNGNGC NNNNANNNGA NTCNNTGTGC TGGCGTTCNA ATTTCGCAGC    61 AGCGGTTTCT TTACCAGACT CGAGGGTACC GACGTCTCAT AAAACATGAT CATCAGGATG   121 GTAGAGTTCA CTCATCAGGC GGTAGATGTA GGAAGGTGCA TTCCCACCAA TGTTGGAGAT   181 AAAGAGGGTA ATGAGCTCTG GGGGAACGTA GTCAAACACA GGGCAATGCA CGCTGACCTT   241 CTCCAGAATG TCCCCTTCTG TGAATGGCAG GACTTCTTCA GGAGCCACAA ACTTATGAAA   301 TGAGTCTTCT TCATTGGGGA ACTGTGGAGA AAGTTTGAAC ATAGGTGCAC AGACGATGAG   361 TGGGGTGGAA TGGTGTTTTG CTGCCAGTGC CAGAGTGTGA GTTCCTGTCA CAGCTCTCAG   421 GGCCCCATTG GCCAGGATGG TCTTCGTGCC AATGATCACC TTGTTGACTC TTGACATAAC   481 GGCAAAAATG GCAGCATCAG TCATGACAGT TGTCTCAATA CCTGCTTTGG ACAAATTCAC   541 AGCCATTTCA TGACCCTGGC AGAAAGGAGC ACACTCTGCT ACAATGACAT GGAATTTCCT   601 CTTTCGGGCA GCCTCTTTGA GGAAGGCCTC TACTGTTCGG GAGAAGCCAA TGGTCATGAT   661 CACCTCATTG GAGTGAATGT GCTCCAGAGC CTGGGCTGCA ATGTTCTCCA TTGTCCCTTC   721 CAGCTCCACT AGCAGCTCAT TAATCGCCTC NATGATGTTG GACTGGAGTT GGGCNTAATG   781 GAAGCTGAAA TCCTCGTTTA NGCCTCCGGA NGNCAACAGT TTGTGCAGGG ACTCCTGCTG   841 ATCACTCTCG TCGCTGCNNC CATGGANTCT GCCATACTCC NNCNGGATAA TCTNGANCAN   901 TCNNCGCACN NNNTNGCCCA CGGNNGNTCT CGGANGGCTG ANCNGCNNNN ATNNNNNNNN 961 CNNNNNNNNN GNATCNNNNN CNNNNNNNNC CNNCNNNNNN NNNN //
Primer: pET_lacIpro_F2 Region: lacI pro, lacI Sequence file: RDB17723_B0Add.seq
>D06721_A2_B0Ad_2_pET_lacIpro_F2_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGCGGT TGCTGGCGCC TATATCGCCG ANATCNCCGA    61 TGGGGAAGAN CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT   121 GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC   181 GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA   241 TAAGGGAGAG CGTCGAGATC CCGGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG   301 CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT   361 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG   421 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT   481 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG   541 CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG   601 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT   661 GTAAAGCGNC GGTGCACAAT CTTCTCGCGC ANCGCGTCAG TGGGCTGATC ATTAACTATC   721 CGCTGGATGA NCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT   781 TTCTTGATGT CTCTGANCAG ACACCCATCA ACAGTATTAN TTTCTCCCAT GAAGANNGTA   841 CGCGACTNNN CGTGGNNCAT CTGGNCGCAT NGGGNCACCA GCAAATCGCG CTGTTAGCGG   901 GNCCNTTAAN TTCTGTCNCN GCGCNNNTGC NNNNGGCTGG CTGGCATAAA TATNNNANNN 961 GNNANTCNAA TTNNNNNNAN NNNGGNNNNG GNNANGGCNN NNNGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl