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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2alpha (#RDB17725)

Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha).

Alternative name eIF2alpha
Clone info. Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha) cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name human EIF2S1 cDNA
Depositor|Developer Ito, Takuhiro |
 
Sequence (partial) RDB17725pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17725 pEBMulti-Neo-human-eIF2alpha DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEBMulti-Neo-human-eIF2alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17725).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17725_A9Kcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 (Pr0495)
Region: CAG pro, insert 5'
Sequence file: RDB17725_A9Kca.seq check
>D06721A4_A9Kc_1_pCAG-F_V3_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTN NNNNNNNNNN GGNNNGNCGG GGTTCGGCTT CTGGCGTGTG
   61 ACCGGCGGCT CTANNNCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
  121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT AACCCTCACT
  181 AAAGGGGTAC CGGGCCCCCC CTCGAGGTCG ACGCCACCAT GCCGGGTCTA AGTTGTAGAT
  241 TTTATCAACA CAAATTTCCT GAGGTGGAAG ATGTAGTGAT GGTGAATGTC AGATCCATTG
  301 CTGAAATGGG GGCTTATGTC AGCTTGCTGG AATACAACAA CATTGAAGGC ATGATTCTTC
  361 TTAGTGAATT ATCCAGAAGG CGTATCCGTT CTATCAACAA ACTCATCCGA ATTGGCAGGA
  421 ATGAGTGTGT GGTTGTCATT AGGGTGGACA AAGAAAAAGG ATATATTGAT TTGTCAAAAA
  481 GAAGAGTTTC TCCAGAGGAA GCAATCAAAT GTGAAGACAA ATTCACAAAA TCCAAAACTG
  541 TTTATAGCAT TCTTCGTCAT GTTGCTGAGG TGTTAGAATA CACCAAGGAT GAGCAGCTGG
  601 AAAGCCTATT CCAGAGGACT GCCTGGGTCT TTGATGACAA GTACAAGAGA CCTGGATATG
  661 GTGCCTATGA TGCATTTAAG CATGCAGTCT CAGACCNATC TATTTTGGAT AGTTTAGATT
  721 TGAATGAAGA TGAACGGGAA GTACTCATTA ATAATATTAA TAGGCGCTTG ACCCCACAGG
  781 CTGTCAAAAT TCGAGCAGAT ATTGAAGTGG CTTGTTATGG TTATGAANGC ATTGATGCTG
  841 TAAAAGAAGC CCTAAGAGCN NGTTTGAATT GTTCTACAGA AAACATGCCN ATTAAGANTA
901 ATCTAATAGC TCNNNCNNNG NATGTNNTGN CTACNNNNNN NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40 pA, insert 3'
Sequence file: RDB17725_A9Kcb.seq check
>D06721A4_A9Kc_1_SV40polyA_R2_H05_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTGCATTC NTTTTATGTT
   61 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
  121 TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTTAATCTT CAGCTTTGGC TTCCATTTCT
  181 TCTGCATCAT CATCTCCATC CACTTCGGCA TTTTCTCTTT CAAGCCTCTC CATCTGCCTC
  241 GCAAGTTCAG TCTCATCTGT ATCTGTGACC ACTTTGGGCT CCATTTGAAC ATTGAACACA
  301 CCCCTCTTTT CCTCAATCTT CTCTTTGATA ACAGCCATAG CTTGACTGAG GACAGAAAGG
  361 CCTTCTGTTC TCTCCAGGGT TGTCGTAGTC ATTACATACC GAGGAGGAGC TATTAGATTA
  421 ATCTTAATGG GCATGTTTTC TGTAGAACAA TTCAAACCTG CTCTTAGGGC TTCTTTTACA
  481 GCATCAATGC CTTCATAACC ATAACAAGCC ACTTCAATAT CTGCTCGAAT TTTGACAGCC
  541 TGTGGGGTCA AGCGCCTATT AATATTATTA ATGAGTACTT CCCGTTCATC TTCATTCAAA
  601 TCTAAACTAT CCAAAATAGA TGGGTCTGAG ACTGCATGCT TAAATGCATC ATAGGCACCA
  661 TATCCAGGTC TCTTGTACTT GTCATCAAAG ACCCAGGCAG TCCTCTGGAA TAGGCTTTCC
  721 AGCTGCTCAT CCTTGGTGTA TTCTAACACC TCAGCAACAT GACGAAGAAT GCTATAAACA
  781 GTTTTGGANT TTGTGAATTT GTCTTCACAT TTGATTGCTT CCTCTGGANA AACTCTTCTT
  841 TTTGACAAAT CNATATATCC TTTTTCNTTG TCCACCNTAA TGACAACCAC ACACTCATTC
  901 CTGCCAATTC NGATGANTTT GTTGATANAN NGGANACNCC NTNNTGGANA NTTCNCTNNN
  961 ANNAANCNTG CCTNNNNTGN TGNTGTATTC CANCAANCTG ANNTAAGCCC CNNNTTTCNN
1021 NNNNGNANNT GANANTNNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB17725_A9Kcc.seq check
>D06721A4_A9Kc_1_SV40pro_ori_F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNGNCGCCT CNGCCTCTGN NCTANTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAGATCATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
  121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
  181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
  241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA
  301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
  361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
  421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
  481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
  541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
  601 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA
  661 NGNTCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG
  721 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATAGC
  781 GTTGGCTANC CGNGANATTG CTGAAGANCT NNGCGGCGAA TGGGCTGANN GCTNCCNCNN
  841 NCTTTACNGN ATCGCNGCTN CNNATTCNNA GCGNNTCGNC NNTCNATCNN CNTTNNNNNC
901 NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]