Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEBMulti-Neo-human-CDC123

Expression vector of human cell division cycle 123 (CDC123).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17728_B0Jup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 Region: inser 5' Sequence file: RDB17728_B0Jua.seq
>D06721A7_B0Ju_3-3_pCAG-F_V3_A07_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCANGNC GGGGTTCGGC TTCTGGNNNG    61 TGANCGGCGG CTNNNNNNNN NNNGNNNNCC ATGTTCATGC CTTCTTCTTT TTCCTACAGC   121 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTAACCCTCA   181 CTAAAGGGGT ACCGGGCCCC CCCTCGAGGT CGACGCCACC ATGAAGAAGG AGCATGTGCT   241 TCACTGCCAG TTCTCCGCGT GGTACCCGTT CTTCCGAGGC GTTACCATCA AGAGTGTCAT   301 TCTTCCACTT CCTCAGAATG TGAAGGATTA TTTACTCGAT GATGGAACTC TGGTGGTTTC   361 AGGAAGGGAT GATCCACCAA CACATTCTCA GCCAGACAGT GATGATGAAG CAGAAGAAAT   421 ACAGTGGTCT GATGATGANA ACNCAGCCAC GCTTACGGCA CCAGAATTTC CTGAGTTTGC   481 CACTAAAGTC CAGGAAGCTA TCAATTCCCT CGGGGGCAGT GTCTTTCCTA AGCTTAATTG   541 GAGTGCCCCA AGGGATGCGT ATTGGATAGC AATGAATAGT TCTCTGAAAT GTAAAACCCT   601 CAGCGACATC TTTCTGCTTT TCAAGAGTTC CGATTTCNTN NNNCGTGACT TCNNTCNNCC   661 GTTTATTCNT TGTACTGATG ATTCTCCNNA TCCATGTATA GAATATGAGC TCGTTCTCCG   721 AAAATGGNGT GAATTGATTC NNNGGGGCTG AGTTTCNATG TTTTGTCAAG GAAAACNAGC   781 TTANTGGTAT TTCTCNAAGA GANTACNCAC NATACTATGN ATCNNNNTNT CTAANCAAAA   841 GNNAANAANN TNCGCANANG CATANCAANN NTTTTTTCNA NGNAACANAT NNNNNACANA   901 NTNNNNNNNA TGAANANTTT NNTGNTNNNA NANNTANNNA NACNNNNNNG GGGAANGNGN   961 GGNNNNNTNN NNTTTNANNN CNNNTNGNNN NANNNNNNAN NNTNNNNNTG NNNNNNNNNC 1021 NNNGNNAGNA NNNGNNNNNN NNNN //
Primer: SV40polyA_R2 Region: SV40 pA, insert 3' Sequence file: RDB17728_B0Jub.seq
>D06721A7_B0Ju_3-3_SV40polyA_R2_B07_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ACAATTGCAT TCNTTTTATG    61 TTTCNGGTTC AGGGGGAGNN NNNNNNGGTT TTNNNNNNCA AGTAAAACCT CTACAAATGT   121 GGTATGGCTG ATTATGATCT AGAGTCGCGG CCGCTCAGTC GTCCTCCTGC TGATTTCTCT   181 TCAGCTTAAG GAAGTCTATT AGCTTGTGAG CGTCCTCCCC AGTAGAGAGG TCTACAAAGT   241 CCTTGGGTAG CCGGTAACTC AAATAGGGGC TGGGCTGGAC TGTCACTTCA CTGTTTGTGC   301 AACGGAAAGC TGGGGAATCC TGCTCTTGAG CGTCAACTTC ACTAAAATCG CCGTTTAAGT   361 TGTTCTCAGA TATCAGTTCT TCCCAGGTGA ACAGCAGTGA ATCTGTGACT TCACCAAATG   421 GATTAAAGTC AATGAGCCAC ACCTTCCCCC TACTGTCTCT GTATATATCG AACACAAAGT   481 CTTCATCTAA GAATTTGTAC TGTATGTGTT TCTTGAAAAA GTCTTGTATG CATCTGCGAA   541 TTTCTTCCTT TTGTTTAGAA ATATGATCAT AGTATTGTGT GTAGTCTCTT TGAGAAATAC   601 CAATAAGCTT GTTTTCCTTG ACAAAACATC GAAACTCAGC CCCAGGAATC NATTCACACC   661 ATTTTCGGAG AACGAGCTCA TATTCTATAC ATGGATCTGG AGAATCNNNN NNACAATGAA   721 TAAACGGCTG ANTGAAGNNN NNAGTGATGA ANTCGGAACT CTTGAAAAAG CAGAAAAGAN   781 GTCNNTGANG NNTTTACATT TCNNANANCT ATTCATTGCT ATCCNATACN CATCCCTTGG   841 GGCACTNCNA TTANNCTTAN GAAAGANACT GNCCCCCNAN GNNNNNNNAT AGCTTCCTGG   901 ACTTTANTGG CAAACTNNNN AAANTNCNGG NNGNCGNAAG CNNGGNNTGN GGTNCTCNTC   961 NTCANANCNN NNNNATTTTC NTNCNNGNNT NNNNNNNNNC ACNNGNNTGN NNNANNNNNN 1021 NNTTNNNNGN AATNNNNNNC NTTNNNCNNN NAANCNNCNN NNANNTNNNN NNNNNN //
Primer: oriP_F Region: EBNA-1 Sequence file: RDB17728_B0Juc.seq
>D06721A7_B0Ju_3-3_oriP_F_D07_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGGGNNNNN NNNNTTCGCN GCTNGTCCTT    61 TTCCTGCTGG TTGCTCCCNN NNNNNNNNNN ATTTAAGGAG GCCAGGCTAA AGCCGTCGCA   121 TGTCTGATTG CTCACCAGGT AAATGTCGCT AATGTTTTCC AACGCGAGAA GGTGTTGAGC   181 GCGGAGCTGA GTGACGTGAC AACATGGGTA TGCCCAATTG CCCCATGTTG GGAGGACGAA   241 AATGGTGACA AGACAGATGG CCAGAAATAC ACCAACAGCA CGCATGATGT CTACTGGGGA   301 TTTATTCTTT AGTGCGGGGG AATACACGGC TTTTAATACG ATTGAGGGCG TCTCCTAACA   361 AGTTACATCA CTCCTGCCCT TCCTCACCCT CATCTCCATC ACCTCCTTCA TCTCCGTCAT   421 CTCCGTCATC ACCCTCCGCG GCAGCCCCTT CCACCATAGG TGGAAACCAG GGAGGCAAAT   481 CTACTCCATC GTCAAAGCTG CACACAGTCA CCCTGATATT GCAGGTAGGA GCGGGCTTTG   541 TCATAACAAG GTCCTTAATC GCATCCTTCA AAACCTCAGC AAATATATGA GTTTGTAAAA   601 AGACCATGAA ATAACNNACA ATGGACTCCC TTANCGGGCC AGGTTGTGGG CCGGGTCCAG   661 GGNNNNNNCC NAAGGGGAGA CGACTCNANT GGTGTAAGAC GACATTGTGG AATAGCNANG   721 GGCNGTTCCT CGCCTTANGT TGTAAAGGGA GGTCTTACTA CCTCCNTATA CNAACACANC   781 GGCNANCCAA GTTCCTTCGN CGGNAGTCCT TTCTANNTGA CTCCTANNCN NNNANAGCTC   841 TTNANANCNT TCTGCAATGN NCTCNAAATT TCGGGTTGNA ANCTCCTTGA NNNNNATGNN   901 TTTNCNAACC ACCCNNCNTT TTTGNNNCNN GNCTCCATNN NCCNNNNCCN NGGGNNNNNN   961 NGCTNNGGNN TTCTNNNNNG NNNNNCNGNN GGGGCCCNGN TCNNNNCGNN NCCNNGNNNN 1021 NACNNNNNGG NNNNNNCCNN NNTNGGGCNN NNCNTNNNNN NNNNN //
Primer: SV40pro_ori_F Region: NeoR_KanR Sequence file: RDB17728_B0Jud.seq
>D06721A7_B0Ju_3-3_SV40pro_ori_F_C07_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNCTCGGC CTCTGNNCTN NTCNNGAAGT AGTGAGGAGG    61 CTTTTTTGGA GGNNNNNNNN NTTGCANNGA TCATCAAGAG ACAGGATGAG GATCGTTTCG   121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT   181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC   241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT   301 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT   361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA   421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT   481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG   541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA   601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA   661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA   721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGNNGGANC GCTATCANGA   781 CATANCGNNG GCTACCCGTG ATATTGCTGA ANGANCTTGG CGGNGAATGG GCTGACCGCT   841 TCNNCGTGCT TTACGGTATC GCCGCTCCCN ANTNNGCAGC GCATCNCCTN NTATCGCNTT   901 CTTGANNAGT TCTNNTGANC NGGNNNTCTG GGGGTTCNAA ATGNCNGANN NAGNGACNNC   961 CANNCTGNNN TCACGAGNNT TCNANTNCNC CNNCNNCNTT CTATGAAANN NNNGNNNTNN 1021 NGNNNNNNNT TTNNNNGNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.27

GNP_filter3_RDBDEP_html_220727.pl