Resource data sheet
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pT2MP.EF-ITPKA-pHlu (#RDB17734)

pH measurement, F-actin, Tol2 transposon

Alternative name pT2MPEF-ITPKA-pHlu (stock no. 1044)
Clone info. pH measurement, F-actin (ITPKA), Tol2 transposon. EF promoter.
Comment Expression was confirmed by the depositor in mammalian cell.
Vector backbone pT2+MCS (plasmid)
Gene/insert name Aequorea victoria GFP cDNA Rat Itpka cDNA
Depositor|Developer Okada, Yasushi | Yaginuma, Hideyuki |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. The BIOLOGICAL RESOURCE shall be transferred to a not-for profit organization. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Koichi Kawakami of the National Institute of Genetics (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004))is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること 。本件リソースは非営利機関の学術研究目的にのみ利用することができる。
MTAに書く付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。利用者は、研究成果の公表にあたって国立遺伝学研究所 川上浩一博士が指定する文献 (Urasaki,A. et al., Genetics 174, 639-649 (2006); Kawakami, K. et al., Developmental Cell 7: 133-144 (2004)) の引用を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17734 pT2MP.EF-ITPKA-pHlu Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pT2MP.EF-ITPKA-pHlu was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17734).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

reference Miesenböck, G., Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394 (6689): 192-195 (1998). PMID 9671304.