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CSII-EF-IRES-GFP-RfA (#RDB17868)

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-GFP-RfA.

Alternative name CS-EF-IG-RfA
Clone info. Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-GFP-RfA.
Vector backbone CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, Chloramphenicol, 30oC
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Fujii, Masahiro | Higuchi, Masaya |
 
Sequence (full) RDB17868zzk03.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者（連絡先：新潟大学 ip@adm.niigata-u.ac.jp）と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
付加的使用条件：
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17868 CSII-EF-IRES-GFP-RfA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-IRES-GFP-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17868).

Reference section:

Higuchi, M., Tsubata, C., Kondo, R., Yoshida, S., Takahashi, M., Oie, M., Tanaka, Y., Mahieux, R., Matsuoka, M., Fujii, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17868_A9Lhp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1a pro, RfA 5' (attR1, CmR)
Sequence file: RDB17868_A9Lha.seq check
>D6420A3_B0Cb_1_EF1a-C_F_A05_02_ABI24.ab1
    1 NNNNNNNNTN NNNNTTTTGA NTTTGGNNCT TGGTTNNTTC TCNAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGCT ACCGGTCTCG AGAATTATCA
  121 CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA
  181 ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC
  241 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT
  301 GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA
  361 AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA
  421 GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC
  481 CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT
  541 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT
  601 GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC
  661 ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA
  721 TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGNTTTATTG AGAATATGTT
  781 TTTCGTCTCA GCCAATCCCT GNGTGAGTTT CACCAGTTTT GANTTAAACG TGGCCNATAT
  841 GGACAACTTC TTCNCCCCCG TTTTCNCCAT GGGCAAATAT TATACGCAAG GNGACAAGGT
  901 GCTGANGNCG CTGGNNANTC NNNNCATCAT GNCGTTTGNG ANGGCTTNCN TGTCNGCANA
  961 ATGCTTANTG NATTACNNCA GTNNTGNGAT GANTGNNNNG GNNGGGCGNA AACNCNNNNN
 1021 NCCNNNTTNN TAAAANNNAN ANANNGTATG CGNATTTGNN NNNNNNNTTN NNNGNNNNNN
1081 NNNN
//
Primer: ccdB-M_F (Pr0951)
Region: RfA 3' (ccdB, attR2), IRES
Sequence file: RDB17868_A9Lhb.seq check
>D06420A3_A9Lh_1_ccdb-M_F_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNGGCTGNN CTCNGCCNCC GCGAAANTGA NATCAAAAAC
   61 GCCATTAACC TGATGTTCTG GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT
  121 GCAGGTCGAC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT
  181 ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT
  241 CTTGTACAAA GTGGTGATAA TTCTGCAGCG GCCGCTACGT AAATTCCGCC CCCCCCCCCC
  301 CTCTCNNNNN NCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC
  361 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA
  421 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT
  481 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC
  541 AACGTCTGTA NCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG
  601 CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCNCGT
  661 TGTGAGTTGG ANAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCNAGCGTAT TCAACNAGGG
  721 NNTGANNGAN GCCCANANNG TACCCCATTG TATGGGNTCT GATCTGGGGC CTCGGTGNAC
  781 ATGCTTTACA TGTGTTTAGT CNAGGTTAAA AAAANGTCTA NGCCCCCCCN ANNCANNGGN
  841 NNGTGNTTTT CCTTTGAAAA ANNCNANGAN NANNATNGNN CNCNNNCNAT GGNNNNNCNN
901 NNNNNNNGAN CTGTNNNNCN GGNNNNNNNN NN
//
Primer: IRES-F (Pr0036)
Region: IRES, EGFP 5'
Sequence file: RDB17868_A9Lhc.seq check
>D6420A3_B0Cb_1_IRES-F_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGNNG TTANNAAACG TCTAGGCCCC CCGAACCACG
   61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
  121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  781 GTGANCGCCG CCNGGATCAC TCTCGGCATG GACNANCTGT ACAAGTAAGT CAACATCGAG
  841 GGATCAAGCT TATCGATAAT CNACCTCTGG ANTACAAAAT TTGTGAAANA NTGACTGGNA
  901 TTCTTANNTA TGTTGCTCCT TTTANNCTAT GTGNANACGC TGCTTTAATG NNTTTGTATC
  961 ATGCTATNGC TNCCCGTATN NNTTTCNNTT TCTCCNCCTT GNNTAANTCC NNNNNGNTGT
1021 CNCNTNATNA NNANNNGNNG NNCNGNTNNN CNNNNANNNN NNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE, EGFP 3'
Sequence file: RDB17868_A9Lhd.seq check
>D6420A3_B0Cb_1_PRE_rev_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNCGNAAA NGGANCNNNN TAGTTAAGAA TACCAGTCAA TCTTTCNCAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTG ACTTACTTGT
  121 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
  181 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
  241 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
  301 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT
  361 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA
  421 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
  481 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
  541 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
  601 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC
  661 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTANGTGG
  721 CATCGCCCTC GCCCTCGCCG GANACGCTGA ACTTGTGGNC GTTTACGTCG CCGTCCAGCT
  781 CGACCAGGAN GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTTGTGG
  841 NCATATTATC ATCGTGTTTT TCAAAGGAAA ACCACGTCCC CGNGGTTCGG GGGGNCTANA
  901 NGTTTTTTNA ACCTCNANTA AACACNTGTA AAGCATGNGC ACCGAGGNCC CANATCANAT
  961 CCCATACANT GGGGTACCTT CTGGGNANNC TNCNNCCCNN NGNTNNNNNN NCTTGANNAN
1021 ANNNNTTNNN NNNNTTCNNN NNCTATNNNN CNNNNNNNNN N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, 5' LTR, HIV-1 psi
Sequence file: RDB17868_A9Lhe.seq check
>D6420A3_B0Cb_1_CMV_Forward_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GCGCGTTTTG CCTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG
  121 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT
  181 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC
  241 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA
  301 GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA
  361 GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG
  421 AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA
  481 ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC
  541 AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA
  601 CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA
  661 TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG
  721 GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGGG
  781 CCGCTGATCT TCANACCTGG AGGANGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT
  841 AAATATAAAG TAGTAAAAAT TGAACCNTTA GGAGTAGCNC CCACCAAGGC AAAGAGAANA
  901 GTGGTGNANA NAGAAAAAAG ANCANTGGGA ATAGGAGCTT TGTNNCTTGG GTTCTTGGGA
  961 NCAGCNNGAN NCNCTATNGG CGCNGCGTCN NTGANNCTGA NGGNANNNNN CNANANNNNT
1021 ATTGNCNGGN NNNNNGNANC NNCNNNNNNA TTTGNTNNNN GNNNNNNNNN N
//
Primer: RRE_check (Pr0837)
Region: RRE
Sequence file: RDB17868_A9Lhf.seq check
>D6420A3_B0Cb_1_RRE_check_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCTTTTC TTGCTGGTTT TGCGATTCTT CNATTAAGGA
   61 GTGTATTAAG CTTGTGTAAT TGTTAATTTC TCTGTCCCAC TCCATCCAGG TCGTGTGATT
  121 CCAAATCTGT TCCAGAGATT TATTACTCCA ACTAGCATTC CAAGGCACAG CAGTGGTGCA
  181 AATGAGTTTT CCAGAGCAAC CCCAAATCCC CAGGAGCTGT TGATCCTTTA GGTATCTTTC
  241 CACAGCCAGG ATTCTTGCCT GGAGCTGCTT GATGCCCCAG ACTGTGAGTT GCAACAGATG
  301 CTGTTGCGCC TCAATAGCCC TCAGCAAATT GTTCTGCTGC TGCACTATAC CAGACAATAA
  361 TTGTCTGGCC TGTACCGTCA GCGTCATTGA CGCTGCGCCC ATAGTGCTTC CTGCTGCTCC
  421 CAAGAACCCA AGGAACAAAG CTCCTATTCC CACTGCTCTT TTTTCTCTCT GCACCACTCT
  481 TCTCTTTGCC TTGGTGGGTG CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA
  541 TAATTCACTT CTCCAATTGT CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCC
  601 TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT
  661 GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA
  721 TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT
  781 ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT
  841 TGCCCATACT ATATGTTTNA ATTTATATTT TTTCTTTCCC CCTGNCCTTA NCCNAANTTT
  901 TTCCCATCGC GATCTAATTC TCCCCCGCTN NANACTGACG CTCTCNCACC CNNTCTCTCT
  961 CCNTCTANCC TCNGCTAGTC NAANTTTTTN GNNGNANTCN CCAGTCNNCN NCCCNNCNNC
 1021 NTNNNGNCNN GNNNNNNTCA NNNNNNNNAN NCCNGNGNNN NNANNNNNNC NNNNNGNNTN
1081 NCNNTTNNNN NTNNNNNNNC NNNNNNNGNN NNNNNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF1a pro, cPPT_CTS, RRE
Sequence file: RDB17868_A9Lhg.seq check
>D6420A3_B0Cb_1_EF1a-N_R_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGCGNNGT GCGCTCTGCC NNCTGACGGG CACCGGANCC
   61 TCACGAAGCT TGAATTAATT CAAAGTGAAT TTCTGCTGTC CCTGTAATAA ACCCGAAAAT
  121 TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT
  181 CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA
  241 TGAATACTGC CATTTGTAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT
  301 CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC
  361 TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA
  421 ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC
  481 CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT
  541 TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG
  601 TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA
  661 GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG
  721 CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTANGTATC TTTCCACAGC CAGGANTCTT
  781 GCCTGGAGCT GCTTGANGCC NCAGACTGTG ANTTGCAACA GATGCTGTTG CGCCTCAATA
  841 GCCCTCANCA AATTGNTCTG CTGCTGCACT ATACCAGANA ATAATTGTCT GGNCTGNANC
  901 GTCAGCGTCA TTGANGCTGC NCCCATANNG CTTCCTGCNG NTCCCNAGAA CCCNANGAAN
  961 AAANCTNCNA TTCCCACNGN NNTTTTTTCT CTCTGCANNA NNNNNNNNNT TGNNNTNNNN
1021 GNNNNNANNC CTNNNNNNNN NN
//
Primer: BGH_rev2 (Pr0606)
Region: BGH p(A), 3'LTR
Sequence file: RDB17868_A9Lhh.seq check
>D6420A3_B0Cb_1_BGH_rev2_H05_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNGGGGG NNGGGNAACN ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG
  121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
  181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
  241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT
  301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC
  361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC
  421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT
  481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT
  541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT
  601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC
  661 CTGANGTGTG ACTGGAANAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC
  721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GANTGCTCCN TGTTTTTCTA GGTCTCGACN
  781 GNATCNATGC GGGGANGCGG CCCAAAGGGA NATCCNANTC NTCTGANGNC GANNNNAANA
841 NNCNNNANNN NNNNNANANN NNNNANCNNN NCNNNGGNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, Zeo, SV40 p(A)
Sequence file: RDB17868_A9Lhi.seq check
>D06420A3_B0Cb_1_SV40pro_ori_F_F07_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
  121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
  181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
  241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
  301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
  361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
  421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
  481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT
  541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG
  601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
  661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT
  721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
  781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT
  841 CNACCTCTAG CTAGAGCTNG NCGNAATCAT GGNCATAGCT GTTTCCTGTG TGAANTTGTT
  901 ATCCGCTCAC NATTCCNCAC NNCATACGAN NCNGGANGCA TAAAGNGNAA ANCCNNNNNN
  961 NGNCNNANTG NNNGANCNNN NNCNCNNTNA NTTGNNNTNN CNNNCANNNN CNNNNTTNNN
1021 NNNNNNGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]
reference Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936. [link to RRC of NBRP]