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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-IRES2-Bsd-RfA (#RDB17869)

Lentivirus expression vector with Gateway (R) cloning, EF-1 alpha promoter, IRES-Bsd-RfA.

Alternative name CS-EF-IB-RfA
Clone info. Lentivirus expression vector, backbone vector. Gateway (R) cloning. EF-1 alpha promoter, IRES-Bsd-RfA.
Vector backbone CSII-EF-MCS (RDB04378) (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions LB+Ampicillin, Chloramphenicol, 30oC
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name Aspergillus terreus blasticidin S deaminase (BSD) cDNA
Depositor|Developer Fujii, Masahiro | Higuchi, Masaya |
 
Sequence (full) RDB17869zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Niigata University (ip@adm.niigata-u.ac.jp). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Higuti, M. et al. J. Virol. 81(21): 11900-11907, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者（連絡先：新潟大学 ip@adm.niigata-u.ac.jp）と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
付加的使用条件：
レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17869 CSII-EF-IRES2-Bsd-RfA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-IRES2-Bsd-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17869).

Reference section:

Higuchi, M., Tsubata, C., Kondo, R., Yoshida, S., Takahashi, M., Oie, M., Tanaka, Y., Mahieux, R., Matsuoka, M., Fujii, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17869_A9Lhp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1alpha pro, RfA 5' (attR1, CmR)
Sequence file: RDB17869_A9Lha.seq check
>D6420A4_B0Cb_1_EF1a-C_F_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNTTTTNANT TTGGNNCTTG GTTNNTTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AACACGCTAC CGGTCTCGAG AATTATCACA
  121 AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT
  181 TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA
  241 TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT
  301 GGATTTTGAG TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA
  361 AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG
  421 CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT
  481 TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG
  541 CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA
  601 TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT
  661 CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACNCATATAT TCGCAAGATG
  721 TGGCGTGTTA CGGTGAAAAC CTGNCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT
  781 TCGTCTCANC CAATCCCTGG GTGAGTTTCA CCNNNTTTTG ATTTAAACGT GGCCNANNAT
  841 GGACAACTTC TTCGCCNCCG TTTTCNCCAT GGGCANATAT TATACGCAAG GCGANAAGGN
  901 GCTGANGCCG CTNGCGATTC NNGTTCATCA TGCCGTTTGT GANGNNTTCC ATGTCGGCAN
  961 ANNGNTNNAN NGAATTACAN NAGTNNTGCN ANGANTGGNN NNGNCGGGGC NNAAACNCGT
1021 GNNNNCNGNN TTNNTAAAAN CNNNNNANNN NNNNNNN
//
Primer: IRES-R (Pr0037)
Region: IRES2, RfA 3' (attR2, ccdB)
Sequence file: RDB17869_A9Lhb.seq check
>D6420A4_B0Cb_1_IRES-R_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTT AGGGGGGGGG GNNGGANAGG GGCGGATCCG
   61 CGGCCGCTGC AGAATTATCA CCACTTTGTA CAAGAAAGCT GAACGAGAAA CGTAAAATGA
  121 TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA
  181 AACACAACAT ATCCAGTCAC TATGGTCGAC CTGCAGACTG GCTGTGTATA AGGGAGCCTG
  241 ACATTTATAT TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT GATGTCATTT TCGCGGTGGC
  301 TGAGATCAGC CACTTCTTCC CCGATAACGG AGACCGGCAC ACTGGCCATA TCGGTGGTCA
  361 TCATGCGCCA GCTTTCATCC CCGATATGCA CCACCGGGTA AAGTTCACGG GAGACTTTAT
  421 CTGACAGCAG ACGTGCACTG GCCAGGGGGA TCACCATCCG TCGCCCGGGC GTGTCAATAA
  481 TATCACTCTG TACATCCACA AACAGACGAT AACGGCTCTC TCTTTTATAG GTGTAAACCT
  541 TAAACTGCAT TTCACCAGCC CCTGTTCTCG TCAGCAAAAG AGCCGTTCAT TTCAATAAAC
  601 CGGGCGACCT CAGCCATCCC TTCCTGATTT TCCGCTTTCC AGCGTTCGGC ACGCAGACGA
  661 CGGGCTTCAT TCTGCATGGT TGTGCTTACC AGACCGGAGA TATTGACATC ATATATGCCT
  721 TGAGCAACTG ATAGCTGTCG CTGTCAACTG TCACTGTAAT ACGCTGCTTC ATAGCATACC
  781 TCTTTTTGAC ATACTTCNGG TATACNTATC AGTATATATT CTTATACCGC AAAAATCAGC
  841 GCGCAAATAC GCATACTGTT ATCTGGCTTT TANNAANNCC GGANCCACGC GTTTACNCCC
  901 CGCCCTGCCA CTCNTCGCAN TACTGTTGTA ATTCATNNAN CATTCTGCCN ANNNGGNNNN
  961 NCNTCNCAAA CGGCATGATG AACCTGAATC GNNAGCNGCN TCAGCNNNNT NGTCGCNNNG
1021 NGNANANNAT TTNNNNNNNN NN
//
Primer: PRE_rev (Pr0250)
Region: PRE, BSD, IRES2 3'
Sequence file: RDB17869_A9Lhc.seq check
>D6420A4_B0Cb_1_PRE_rev_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNNGGANCN NCATAGTTAA GAATACCNGT CAATCTTTCA
   61 CAAATTTTGT AATCCAGAGG TTGATTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG
  121 AGGCTCGACT TAGCCCTCCC ACACATAACC AGAGGGCAGC AATTCACGAA TCCCAACTGC
  181 CGTCGGCTGT CCATCACTGT CCTTCACTAT GGCTTTGATC CCAGGATGCA GATCGAGAAG
  241 CACCTGTCGG CACCGTCCGC AGGGGCTCAA GATGCCCCTG TTCTCATTTC CGATCGCGAC
  301 GATACAAGTC AGGTTGCCAG CTGCCGCAGC AGCAGCAGTG CCCAGCACCA CGAGTTCTGC
  361 ACAAGGTCCC CCAGTAAAAT GATATACATT GACACCAGTG AAGATGCGGC CGTCGCTAGA
  421 GAGAGCTGCG CTGGCGACGC TGTAGTCTTC AGAGATGGGG ATGCTGTTGA TTGTAGCCGT
  481 TGCTCTTTCA ATGAGGGTGG ATTCTTCTTG AGACAAAGGC TTGGCCATGG TTGTGGCCAT
  541 ATTATCATCG TGTTTTTCAA AGGAAAACCA CGTCCCCGTG GTTCGGGGGG CCTAGACGTT
  601 TTTTTAACCT CGACTAAACA CATGTAAAGC ATGTGTACCG AGGCCCCAGA TCAGATCCCA
  661 TACAATGGGG TACCTTCTGG GCATCCTTCA GCCCCTTGTT GAATACGCTT GAGGAGAGCC
  721 ATTTGACTCT TTCCACAACT ATCCAACTCA CAACGTGGCA CTGGGGTTGT GCCGCCTTTG
  781 CAGGTGTATC TTATACACGT GGCTTTTGGC CGCAGANGCA CCTGTCGCCA GGTGGGGGGG
  841 TTCCGCTGCC TGCAAAGGGT CNCTACAGAC GTTGTTTGTC TTCAAGAANC TTCCNGANGA
  901 NNTGNTTCCT TCNCGACATT CNACAGANCT TGNATTCCTT TGGCGANAGG GGAAAGACCC
  961 CTANGAATGN TCGNCAANAA NANAGGGNNN NGNTTNNCNG GNNNCNCNCA TTGNCAANNN
1021 NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, 5'LTR, HIV-1 psi
Sequence file: RDB17869_A9Lhd.seq check
>D6420A4_B0Cb_1_CMV_Forward_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
  121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
  181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
  241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC
  301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG
  361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG
  421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT
  481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC
  541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC
  601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA
  661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT
  721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
  781 TCTTCAGACC TGGAGGANGA GATATGAGGG ANAATTGGAG AAGTGAATTA TATANATATA
  841 AAGTAGTAAA AATTGNACCN TTAGGANTAG CACCCACCNA NGCAAAGANA AGAGTGGTGC
  901 AGANAGAAAA AAGANCAGTG GGAATAGGAN CTTTGNNCCT NGGGNTCTTG GGANCAGCNN
  961 NAAGCNCTAT GGGCNNANCG NNNNNGACNC NNNNNNNNCN NNNNNGNNNN TNNNNGNCTN
1021 NNNTNNNNNN NNNN
//
Primer: RRE_check (Pr0837)
Region: RRE
Sequence file: RDB17869_A9Lhe.seq check
>D6420A4_B0Cb_1_RRE_check_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNTNNNNNN TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT
   61 GTATTAAGCT TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC
  121 AAATCTGTTC CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA
  181 TGAGTTTTCC AGAGCAACCC CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA
  241 CAGCCAGGAT TCTTGCCTGG AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT
  301 GTTGCGCCTC AATAGCCCTC AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT
  361 GTCTGGCCTG TACCGTCAGC GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCA
  421 AGAACCCAAG GAACAAAGCT CCTATTCCCA CTGCTCTTTT TTCTCTCTGC ACCACTCTTC
  481 TCTTTGCCTT GGTGGGTGCT ACTCCTAATG GTTCAATTTT TACTACTTTA TATTTATATA
  541 ATTCACTTCT CCAATTGTCC CTCATATCTC CTCCTCCAGG TCTGAAGATC AGCGGCCCTT
  601 GCTGTGCGGT GGTCTTACTT TTGTTTTGCT CTTCCTCTAT CTTGTCTAAA GCTTCCTTGG
  661 TGTCTTTTAT CTCTATCCTT TGATGCACAC AATAGAGGGT TGCTACTGTA TTATATAATG
  721 ATCTAAGTTC TTCTGATCCT GTCTGAAGGG ATGNNTGTAG CTGTCCCAGT ATTTGTCTAC
  781 AGCCTTCTGA NGTTTCTAAC AGGNCAGGAN TAACTGCGAA TCGTTCTAGC TCCCTGCTTG
  841 CCCATACTAT ATGTTTTAAT TTATATTTTT TCTTTCCCCC TGGCCTTAAC CNAATTTTTT
  901 CCNNTCGCGA TCNAATTCTC CCCCGCTNAA TACTGACGCT CTCGCACCCN TCTCTCTNNT
  961 TCNANCNNNC GCNAGTCNAA ATTTTNGGNN NANTCACCAN NCGNCNNCCC NNNGCNNNNN
1021 GNNNNGNNNN NNNCANCNNN NCNNANNNNN NNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF-1alpha pro, cPPT_CTS, RRE
Sequence file: RDB17869_A9Lhf.seq check
>D6420A4_B0Cb_1_EF1a-N_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNGCGAN GTGCGCTCTG CCCNCTGACG GGCACCGGAN
   61 CCTCACGAAG CTTGAATTAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA
  121 ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT
  181 GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG
  241 GATGAATACT GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT
  301 CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG
  361 TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA
  421 AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA
  481 GCCAATTTGT TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT
  541 CTTGTTCATT CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAAGGAGTGT ATTAAGCTTG
  601 TGTAATTGTT AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCAA ATCTGTTCCA
  661 GAGATTTATT ACTCCAACTA GCATTCCAAG GCACAGCAGT GGTGCAAATG AGTTTTCCAG
  721 AGCAACCCCA AATCCCCAGG AGCTGTTGAT CCTTTAGGTA TCTTTCCNCA GCCAGGATTC
  781 TTGCCTGGAG CTGCTTGATG CCCCAGACTG TGAGTTGCAA CAGATGCTGT TGCGCCTCAA
  841 TANCCCTCAN CAAATTGTTC TGCTGCTGCA CTATACCANA NAATAATTGT CTGGNCTGNA
  901 CCGNCAGCGT CATTGACGCT GCGCCNNTAG TGCTTCCTGC TGCTCCCAAN AACCCNAGGA
  961 NNAAAGCTCC TATTCCNNCT GNTCTTTTTT NTCTCTGCAN CANNCNTCNN TTTNNCTNNN
1021 NNGGNNNTAN NNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: BGH pA, 3'LTR, PRE
Sequence file: RDB17869_A9Lhg.seq check
>D6420A4_B0Cb_1_BGH_rev2_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGGGNNG GGCANCAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG
  721 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT
  781 CTCGACGGTA TCGATGCGGG GAGGCGGCCC NAAGGGAGAT CCGACTCGTC TGANGGCGAA
  841 NGCGAAGANG CGGAANANGC CGCANANCNG GCAGCANGCC GCGGGAANGA ANGTCCGCTG
  901 GANTGANGGN CGAANGGNNG TANCANAANG NNGTCCCGCG CANAATCNNN GNGGNANCNC
  961 ANGCNANCAG CCNTGGAAAG GANGTCAGCT TNCCNNANNN NCNNCGGNAN TNNNCANTGN
1021 CNNNCAGNCN ANCCCNNNNN NNGNNNCNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, Zeo, SV40 pA
Sequence file: RDB17869_A9Lhh.seq check
>D6420A4_B0Cb_1_SV40pro_ori_F_H06_24_ABI24.ab1
    1 NNNNNNNNNN NNNGCCGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAGCACGTG TTGACAATTA ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG
  181 GTGAGGAACT AAACCATGGC CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC
  241 GTCGCCGGAG CGGTCGAGTT CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG
  301 GACGACTTCG CCGGTGTGGT CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC
  361 CAGGTGGTGC CGGACAACAC CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC
  421 GCCGAGTGGT CGGAGGTCGT GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC
  481 GAGATCGGCG AGCAGCCGTG GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC
  541 GTGCACTTCG TGGCCGAGGA GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC
  601 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
  661 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT
  721 AATGGTTACN AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCNCTG
  781 CATTCTAGTT GTGGTTTGTC CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG
  841 ACNTCTANCT AGAGCTTGNC GTAATCATGG TCATAGCTGN TTCCTGNGTG AAANTGTTAT
  901 CCGCTCACAA TTNCNCACAA CATACNANCN NNAANCATAA AGTGTAAAGC CTGGGGNGNC
  961 TNNNGAGTGA NCTNNCTCNC ANTNNNTNGN NNNGNNNTCN CNGCCNNNTT TCCANNNNGN
1021 NNANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Higuchi, M., Cooperation of NF-kappaB2/p100 activation and the PDZ domain binding motif signal in human T-cell leukemia virus type 1 (HTLV-1) Tax1 but not HTLV-2 Tax2 is crucial for interleukin-2-independent growth transformation of a T-cell line. J. Virol. 81 (21): 11900-11907 (2007). PMID 17715223. [link to RRC of NBRP]
reference Takachi, T., Human T-cell leukemia virus type 1 Tax oncoprotein represses the expression of the BCL11B tumor suppressor in T-cells. Cancer Sci. 106 (4): 461-465 (2015). PMID 25613934. [link to RRC of NBRP]
reference Higuchi, M., Downregulation of proapoptotic Bim augments IL-2-independent T-cell transformation by human T-cell leukemia virus type-1 Tax. Cancer Med. 3 (6): 1605-1614 (2014). PMID 25175936. [link to RRC of NBRP]