RDB18212 - pCAG-All-in-one-hCascade

pCAG-All-in-one-hCascade (#RDB18212)

Expression vector of CRISPR-associated complex for antiviral defense for genome editing tool.

Drawn by SnapGene® software

Sequence (full)	RDB18212hts01.seq
Publication	Morisaka, H., Nat. Commun. 10 (1): 5302 (2019). [link to RRC of NBRP]
Test sheet	RDB18212C0p1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	Expression vector of CRISPR-associated complex for antiviral defense (Cascade; Cse1, Cse2, Cas5, Cas6, Cas7) for genome editing tool, TypeI-E CRISPR/Cas3 system in mammalian cells. A components for genome editing in mammalian cells. Used with pPB-CAG-hCas3 (RDB18213) and pBS-U6-crRNA-empty (RDB18214) containing a target sequence.
Vector backbone	Plasmid
Gene/insert name	E. coli Cas7, Cse2, Cse1, Cas5, Cas6.
Depositor\|Developer	Mashimo, Tomoji \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morisaka, H. et al. Nat. Commun.10(1), 5302, 2019)

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morisaka, H. et al. Nat. Commun.10(1), 5302, 2019)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Morisaka, H. et al. Nat. Commun.10(1), 5302, 2019)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18212	pCAG-All-in-one-hCascade	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAG-All-in-one-hCascade was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18212).

Reference section:

Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., Mashimo, T., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Morisaka, H., CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nat. Commun. 10 (1): 5302 (2019). PMID 31811138. [link to RRC of NBRP]