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CoralHue(TM) monomeric Kusabira-Orange 1 (pmKO1-S1) (#RDB18258)

CoralHue™ monomeric Kusabira-Orange 1 (pmKO1-S1)

Alternative name AM-V0051M
Clone info. ・For subcloning.
・Monomeric Kusabira-Orange1 is Orange fluorescent protein.
・Ex/Em(nm): 548/561
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18258zzk01.seq provided by the depositor
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18258 CoralHue(TM) monomeric Kusabira-Orange 1 (pmKO1-S1) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) monomeric Kusabira-Orange 1 (pmKO1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18258).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18258_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536)
Region: MCS, mKO1 5'
Sequence file: RDB18258_B0H4a.seq check
>D07039B9_B0H4_4_pUC-CAPbs_up_F_C05_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ACGCNANNTA ATGTGANNNN NNTCNCTCNT
   61 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
  121 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGAATTCGA GCTCGGTACC
  181 CGGGGATCCC CACCATGGTG AGTGTGATTA AACCAGAGAT GAAGATGAGG TACTACATGG
  241 ACGGCTCCGT CAATGGGCAT GAGTTCACAA TTGAAGGTGA AGGCACAGGC AGACCTTACG
  301 AGGGACATCA AGAGATGACA CTACGCGTCA CAATGGCCAA GGGCGGGCCA ATGCCTTTCG
  361 CGTTTGACTT AGTGTCACAC GTGTTCTGTT ACGGCCACAG ACCTTTTACT AAATATCCAG
  421 AAGAGATACC AGACTATTTC AAACAAGCAT TTCCTGAAGG CCTGTCATGG GAAAGGTCGT
  481 TGGAGTTCGA AGATGGTGGG TCCGCTTCAG TCAGTGCGCA TATAAGCCTT AGAGGAAACA
  541 CCTTCTACCA CAAATCCAAA TTTACTGGGG TTAACTTTCC TGCCGATGGT CCTATCATGC
  601 AAAACCAAAG TGTTGATTGG GAGCCATCAA CCGAGAAAAT TACTGCCAGC GACGGAGTTC
  661 TGAAGGGTGA TGTTACGATG TACCTAAAAC TTGAAGGAGG CGGCAATCAC AAATGCCAAT
  721 TCAAGACTAC TTACAAGGCG GCAAAAAAGA TTCTTAAAAT GCCNGGAAGC CATTACATCA
  781 NCNNTCGCCT CGTCAGGAAA ACCGAANGCA ACATTACTGA NCTGGTAGAA GANGCANNAN
  841 CTCATTCCTA ACTGCANGCA TGCAAGCTNG GCACTGNNCG TCGTTTTANN ACGTCGNGNC
  901 TGGGAAAANC CNNNNNTNNN CNNACTTNNA NCGCCNNGCN NCNNATNCNN NNNTNNNNNA
961 GCTGGNNNNN NNNCNNANNN GNCCNNNNNN ATCNNNNNNN CNNNNNNNNN N
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, mKO1
Sequence file: RDB18258_B0H4b.seq check
>D07039B9_B0H4_4_pUC-M13_-100_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNANGCNNN GGTTTTCCNN GTCNCGACGT TGTAAAACGA
   61 CGGCCAGTGC CAAGCTTGNA TGCCTGCAGT TAGGAATGAG CTACTGCATC TTCTACCAGC
  121 TCAGTAATGT TGCCTTCGGT TTTCCTGACG AGGCGATGGC TGATGTAATG GCTTCCTGGC
  181 ATTTTAAGAA TCTTTTTTGC CGCCTTGTAA GTAGTCTTGA ATTGGCATTT GTGATTGCCG
  241 CCTCCTTCAA GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA
  301 ATTTTCTCGG TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA
  361 GGAAAGTTAA CCCCAGTAAA TTTGGATTTG TGGTAGAAGG TGTTTCCTCT AAGGCTTATA
  421 TGCGCACTGA CTGAAGCGGA CCCACCATCT TCGAACTCCA ACGACCTTTC CCATGACAGG
  481 CCTTCAGGAA ATGCTTGTTT GAAATAGTCT GGTATCTCTT CTGGATATTT AGTAAAAGGT
  541 CTGTGGCCGT AACAGAACAC GTGTGACACT AAGTCAAACG CGAAAGGCAT TGGCCCGCCC
  601 TTGGCCATTG TGACGCGTAG TGTCATCTCT TGATGTCCCT CGTAAGGTCT GCCTGTGCCT
  661 TCACCTTCAA TTGTGAACTC ATGCCCATTG ACGGAGCCGT CCATGTAGTA CCTCATCTTC
  721 ATCTCTGGTT TAATCACACT CACCATGGTG GGGNNCCCCG GGTACCGAGC TCGAATTCGT
  781 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
  841 TACGAGCCGG NNNCATAAAG TGTAAAGCCT GGGGTGNCTA NTGANTGANC TAACTCACAT
  901 TAATTGCGTT GCGCTCACTG CCCGCTTNCC AGTCNGGAAA NNTGNCNNGN NNNCTGCNNN
961 AANNNNTCNG NCNAACGCNN NGGGNNNANG NNGNNTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]