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CoralHue(TM) Kikume Green-Red 1 (pKikGR1-S1) (#RDB18272)

CoralHue™ Kikume Green-Red 1 (pKikGR1-S1)

Alternative name AM-V0081M
Clone info. ・For subcloning.
・Kikume Green-Red1 is bright green fluorescent protein that can be irreversibly converted to red by UV or violet light(350-410nm).
・Ex/Em(nm): 507/517(Green),583/593(Red)
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Favia favus Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18272zzk01.seq provided by the depositor
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18272 CoralHue(TM) Kikume Green-Red 1 (pKikGR1-S1) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) Kikume Green-Red 1 (pKikGR1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18272).

Reference section:

Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., Miyawaki, A., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18272_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18272_B0H4a.seq check
>D07039D5_B0H4_14_lacIpro-B_F_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN CGNNNCNGTG AGCGNNGANC GGAAGANCGC CCAATACGCA
   61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
  121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
  181 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
  241 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT CGAGCTCGGT ACCCGGGGAT
  301 CCCCACCATG GTGAGTGTGA TTACATCAGA AATGAAGATC GAGCTGCGTA TGGAAGGCGC
  361 TGTAAACGGG CACAAGTTCG TGATTACAGG GAAAGGAAGT GGCCAGCCTT TCGAGGGAAT
  421 ACAGAATGTG GACCTGACAG TCATAGAGGG CGGACCTCTT CCTTTTGCTT TCGATATCCT
  481 GACAACAGCA TTCCATTACG GCAACCGGGT ATTTGTCGAA TACCCAGAAG AAATAGTAGA
  541 CTACTTCAAG CAGTCGTTTC CTGAGGGTTA TTCTTGGGAA CGAAGCATGA GTTACGAAGA
  601 CGGGGGAATT TGCCTCGCCA CAAACAATAT AACGATGAAG AAAGACGGCA GCAACTGTTT
  661 TGTCAATGAA ATTCGATTTG ATGGTGTGAA CTTTCCTGCC AATGGTCCAG TTATGCAGAG
  721 GAAGANCGTC AAATGGGAGC CATCCACTGA GAAAATGTAT GTGCGTGATG GANTGCTGAA
  781 NGGTGANGTA AACATGGCTC TGTTGCTTCN AGGANGTGGC CATTACCGAT GTGACTTCAG
  841 AACTACTTAC NAAGCAAAGA ANGNTGTCCA GTNGNNNGAN NATCACTTCN NGGATCATCN
  901 AANNNNNATN ACAAGCNNNG ANNAGGNNNA NNACNANGNT TNANCTGNNN NANCNCGNNN
961 AAGNNNNNNN CNNGNNNNNN NNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert 3'
Sequence file: RDB18272_B0H4b.seq check
>D07039D5_B0H4_14_pUC-M13_-100_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNANGCCNG GNNTTTCCCN GTCNCGACGT TGTAAAACGA
   61 CGGCCAGTGC CAAGCTTGCA TGCCTGCAGT TACTTGGCCA GCCTTGGCAG CCCGGAATGA
  121 GCTTTAGCGT GCTCATACAG CTTAACCTTG TTGTAATCCT TGTCATGGCT TGTTATCTCC
  181 ATTTGATGAT CCACGAAGTG ATAGTCTGGC AACTGGACAA CCTTCTTTGC TTTGTAAGTA
  241 GTTCTGAAGT CACATCGGTA ATGGCCACCT CCTTGAAGCA ACAGAGCCAT GTTTACATCA
  301 CCCTTCAGCA CTCCATCACG CACATACATT TTCTCAGTGG ATGGCTCCCA TTTGACGGTC
  361 TTCCTCTGCA TAACTGGACC ATTGGCAGGA AAGTTCACAC CATCAAATCG AATTTCATTG
  421 ACAAAACAGT TGCTGCCGTC TTTCTTCATC GTTATATTGT TTGTGGCGAG GCAAATTCCC
  481 CCGTCTTCGT AACTCATGCT TCGTTCCCAA GAATAACCCT CAGGAAACGA CTGCTTGAAG
  541 TAGTCTACTA TTTCTTCTGG GTATTCGACA AATACCCGGT TGCCGTAATG GAATGCTGTT
  601 GTCAGGANAT CGAAAGCAAA AGGAAGAGGT CCGCCCTCTA TGANTGTCAN GTCCACATTC
  661 TGTATTCCCT CNAAAGGNTG GNCACTTCCT TTCCCTGNAA TCNNNAANTT GTGNCCGNNT
  721 ANAGCGNCTT CCNTACNCAG CTCGNTCTTC ATTTCTGANG TANTCACACT CACCATGGTG
  781 GGGNNNCCCN GNTANCGANC TCGAANTCNN ANNCNNGGTC NTANCTGTTT NNNGNGNGAA
  841 ANNGNNATCC NNTCNCNNTN NCNCACNNNN TANNANCNNG NNNNATAAAN NGNAAANNNN
901 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]