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CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1) (#RDB18275)

CoralHue™ humanized Kikume Green-Red 1 (phKikGR1-S1)

Alternative name AM-V0084M
Clone info. ・For subcloning.
・The codons are humanized.
・Kikume Green-Red1 is bright green fluorescent protein that can be irreversibly converted to red by UV or violet light(350-410nm).
・Ex/Em(nm): 507/517(Green),583/593(Red)
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Favia favus Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18275zzk02.seq provided by the depositor
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18275 CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18275).

Reference section:

Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., Miyawaki, A., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18275_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18275_B0H4a.seq check
>D07039D8_B0H4_15_lacIpro-B_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN NCGNNTCNGT GNGCGAGGAA GCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
  121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
  181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG
  301 ATCCCCACCA TGGTGAGCGT GATCACCAGC GAGATGAAGA TCGAGCTGAG GATGGAGGGC
  361 GCCGTGAACG GCCACAAGTT CGTGATCACC GGCAAGGGCA GCGGCCAGCC CTTCGAGGGC
  421 ATCCAGAACG TGGACCTGAC CGTGATCGAG GGCGGCCCCC TGCCCTTCGC CTTCGACATC
  481 CTGACCACCG CCTTCCACTA CGGCAACAGG GTGTTCGTGG AGTACCCCGA GGAGATCGTG
  541 GACTACTTCA AGCAGAGCTT CCCCGAGGGC TACAGCTGGG AGAGGAGCAT GAGCTACGAG
  601 GACGGCGGCA TCTGCCTGGC CACCAACAAC ATCACCATGA AGAAGGACGG CAGCAACTGC
  661 TTCGTGAACG AGATCAGGTT CGACGGCGTG AACTTCCCCG CCAACGGCCC CGTGATGCAG
  721 AGGAAGANCG TGAAGTGGGA GCCCAGCACC GAGAAGANGT ACGTGAGGGA CGGCGTGCTG
  781 AANGGCGACG TGAACATGGC CCTGCTGCTC CAGGGCGGCG GCCACTACAG GTGCGACTTC
  841 NNGNNCACCT ACAANGNCAA GAANGTGGTG CAGCTGCCCG ANTACCACTT CNNNGANNAC
  901 CAGATGGANN TCACCAGCCA CGACAAGGNN TACNACNANN NNGAANCTGN NNNANCACGC
961 CNNNGNCNNC AGCNGNCCNG NCNNNGNNNG GNCNNNNNNN NNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert 3'
Sequence file: RDB18275_B0H4b.seq check
>D07039D8_B0H4_15_pUC-M13_-100_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNANGCNNG GNNTTTCCCN GTCNCGACGT TGTAAAACGA
   61 CGGCCAGTGC CAAGCTTGCA TGCCTGCAGT TACTTGGCCA GCCTGGGCAG GCCGCTGTGG
  121 GCCTTGGCGT GCTCGTACAG CTTCACCTTG TTGTAGTCCT TGTCGTGGCT GGTGATCTCC
  181 ATCTGGTGGT CCACGAAGTG GTAGTCGGGC AGCTGCACCA CCTTCTTGGC CTTGTAGGTG
  241 GTCCTGAAGT CGCACCTGTA GTGGCCGCCG CCCTGGAGCA GCAGGGCCAT GTTCACGTCG
  301 CCCTTCAGCA CGCCGTCCCT CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCACGGTC
  361 TTCCTCTGCA TCACGGGGCC GTTGGCGGGG AAGTTCACGC CGTCGAACCT GATCTCGTTC
  421 ACGAAGCAGT TGCTGCCGTC CTTCTTCATG GTGATGTTGT TGGTGGCCAG GCAGATGCCG
  481 CCGTCCTCGT AGCTCATGCT CCTCTCCCAG CTGTAGCCCT CGGGGAAGCT CTGCTTGAAG
  541 TAGTCCACGA TCTCCTCGGG GTACTCCACG AACACCCTGT TGCCGTAGTG GAAGGCGGTG
  601 GTCAGGATGT CGAAGGCGAA NGGCAGGGGG CCGCCCTCGA TCACGGTCAG GTCCACGTTC
  661 TGGATGCCCT CGAAGGGCTG GCCGCTGCCC TTGCCGGTGA NCACGAACTT GTGGCCGTTC
  721 ACGGCGCCCT CCATCCTCAG CTCGATCTTC ATCTCGCTGG TGATCACGCT CACCATGGTG
  781 GGGATCCCCG GGTACCGAGC TCGAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
  841 NTTGTTATCC GCTCACAATT CCNCACAACA TACGANCCGG AANCATAAAG TGTAAAGCCT
  901 GGGGNGNCTA ATGANTGANC NAACTCACAT TAATTGCGTT GCGCNNNCTG NCNGCTTNNC
  961 ANNNNGNNAA CCNNNCNNNN CANCNNCNTT AANGAANNNN GNCNACGNNN NGGNNNNNNN
1021 NGNNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765. [link to RRC of NBRP]