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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1)

CoralHue™ β-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1)

Catalog number RDB18314
Resource name CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1)
Alternative name AM-V0205M
Clone info. ・For mammalian expression of the β‐Actin-targeted fluorescent protein.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone pActin (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Galaxea fascicularis green fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18314zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18314_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
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            提供同意書 (MTA, 営利目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18314 CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18314).

            Reference section:

            Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18314_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro,insert (mAG1 5')
            Sequence file: RDB18314_B0H4a.seq check
            >D07039I2_B0H4__2_CMV_Forward_A06_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNT GNNGCNNATG GGCGGTANGC GTGTACGGTG GGAGGTCTAT
               61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC ATTGCCACCA TGGTGAGTGT
              121 GATTAAACCA GAGATGAAGA TCAAGCTGTG TATGAGAGGC ACTGTAAACG GGCATAATTT
              181 CGTGATTGAA GGAGAAGGAA AAGGAAATCC TTACGAGGGA ACGCAGATTT TAGACCTGAA
              241 CGTCACTGAA GGCGCACCTC TGCCTTTCGC TTACGATATC TTGACAACAG TGTTCCAGTA
              301 CGGCAACAGG GCATTCACCA AGTACCCAGC AGATATTCAG GACTATTTCA AGCAGACTTT
              361 TCCTGAGGGG TATCACTGGG AAAGAAGCAT GACTTATGAA GACCAGGGCA TTTGCACCGC
              421 CACAAGCAAC ATAAGCATGA GGGGCGACTG TTTTTTCTAT GACATTCGTT TTGATGGCAC
              481 CAACTTTCCT CCCAATGGTC CGGTTATGCA GAAGAAGACT CTTAAATGGG AGCCATCCAC
              541 TGAGAAAATG TACGTAGAGG ATGGAGTGCT GAAGGGTGAT GTTAACATGC GCCTGTTGCT
              601 TGAAGGAGGT GGCCATTATC GATGTGATTT CAAAACTACT TACAAAGCAA AGAAGGAGGT
              661 CCGTTTGCCA GACGCGCACA AAATTGACCA CCGCATTGAG ATTTTGAAGC ATGACAAAGA
              721 TTACAACAAG GTCAAGCTCT ATGAGAATGC CGTTGCTCGC TATTCTATGC TGCCGAGTCA
              781 GGNCAAGACC GGTAATTCCG CTGACGGCGG CGGANGATCG GGTGGTAGTG GTGGTTCAGG
              841 ANGAGGANCG ANCCAAGGAN GNTCCATGGA TGANGATATC GCCGCGCTCG NCGTCGANNA
              901 NNGCTCCGGN ATGTGCAAGG NCGGNTNCGC GGGCGANNAT GCCCCCCGGG CCGTCTTCCC
              961 CNCCATCGNG GGGCGCCCCN NNNCNNNGGN NTGATGNNGG GNATGGGNCA NANNNANTCC
            1021 TATGTGGNNN NNNANNNCNN NNNNNNNN
            //
            Primer: SV40polyA_R2 (Pr0623)
            Region: insert (hActin beta 3')
            Sequence file: RDB18314_B0H4b.seq check
            >D07039I2_B0H4__2_SV40polyA_R2_B06_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAA CAACAANNAT TGCATTCNTT TTATGTTTCA
               61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
              121 GGCTGATTAT GATCTAGAGT CGCGGCCGCT TAGAAGCATT TGCGGTGGAC GATGGAGGGG
              181 CCGGACTCGT CATACTCCTG CTTGCTGATC CACATCTGCT GGAAGGTGGA CAGCGAGGCC
              241 AGGATGGAGC CGCCGATCCA CACGGAGTAC TTGCGCTCAG GAGGAGCAAT GATCTTGATC
              301 TTCATTGTGC TGGGTGCCAG GGCAGTGATC TCCTTCTGCA TCCTGTCGGC AATGCCAGGG
              361 TACATGGTGG TGCCGCCAGA CAGCACTGTG TTGGCGTACA GGTCTTTGCG GATGTCCACG
              421 TCACACTTCA TGATGGAGTT GAAGGTAGTT TCGTGGATGC CACAGGACTC CATGCCCAGG
              481 AAGGAAGGCT GGAAGAGTGC CTCAGGGCAG CGGAACCGCT CATTGCCAAT GGTGATGACC
              541 TGGCCGTCAG GCAGCTCGTA GCTCTTCTCC AGGGAGGAGC TGGAAGCAGC CGTGGCCATC
              601 TCTTGCTCGA AGTCCAGGGC GACGTAGCAC AGCTTCTCCT TAATGTCACG CACGATTTCC
              661 CGCTCGGCCG TGGTGGTGAA GCTGTAGCCG CGCTCGGTGA GGATCTTCAT GAGGTAGTCA
              721 GTCAGGTCCC GGCCAGCCAG GTCCAGACGC AGGATGGCAT GGGGGAGGGC ATACCCCTCG
              781 TAGATGGGCA CAGTGTGGGT GANCCCGTCA CCGGANTCCA TCACGATGCC AGTGGTACGG
              841 CCAGAGGCGT ACAGGGNTAG CACAGCCTNN NTAGCNACGT ACATGGCTGG GGTGNTGAAN
              901 GTCTCAAACA TGATCTGGGN CATCTTCTCG CGNTTGGCCT TGGGGTTCNN NGGGCCNCNG
              961 NCANCANCAN GGGGNGNTNN TNGGGANNCN CANNCNNCNC NNNNNANNNG NNNNNNNGNC
            1021 NNANTTTNNN NNNNNNN
            //
            Primer: mAG_MF1 (Pr1043)
            Region: insert mid(mAG1 3',linker,hActin beta 5')
            Sequence file: RDB18314_B0H4c.seq check
            >D07039I2_B0H4__2_mAG_MF1_D06_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN CNNTAGAGGN NGGAGTGCTG AAGGGTGANG
               61 TTAACATGCG CCTGTTGCTT GAAGGAGGTG GCCATTATCG ATGTGATTTC AAAACTACTT
              121 ACAAAGCAAA GAAGGAGGTC CGTTTGCCAG ACGCGCACAA AATTGACCAC CGCATTGAGA
              181 TTTTGAAGCA TGACAAAGAT TACAACAAGG TCAAGCTCTA TGAGAATGCC GTTGCTCGCT
              241 ATTCTATGCT GCCGAGTCAG GCCAAGACCG GTAATTCCGC TGACGGCGGC GGAGGATCGG
              301 GTGGTAGTGG TGGTTCAGGA GGAGGATCGA CCCAAGGAGG ATCCATGGAT GATGATATCG
              361 CCGCGCTCGT CGTCGACAAC GGCTCCGGCA TGTGCAAGGC CGGCTTCGCG GGCGACGATG
              421 CCCCCCGGGC CGTCTTCCCC TCCATCGTGG GGCGCCCCAG GCACCAGGGC GTGATGGTGG
              481 GCATGGGTCA GAAGGATTCC TATGTGGGCG ACGAGGCCCA GAGCAAGAGA GGCATCCTCN
              541 NCCTGAAGTA CCCCATCGAG CACGGCATCG TCACCAACTG GGACGACATG GAGAAAATCT
              601 GGCACCACAC CTTCTACAAT GAGCTGCGTG TGGCTCCCGA GGAGCACCCC GTGCTGCTGA
              661 CCGAGGCCCC CCTGAACNNN NNNGCCAACC GCGAGAAGAT GACCCAGATC ATGTTTGAGA
              721 CCTTCAACAC CCCAGCCATG TACGTTGCTA TCCAGGNNGN NCNNNNCCTG TNCGCCTCTG
              781 GCCGTACCAC TGNNNNCGTG ATGGACTCCG GTGANGGGGT CACNCACNCT GTGCCCATCT
              841 ACGANGGGTA TGCCCTCCNN ATGCCATCCN GCGTCTGGAN CTGNCTGGNC NGGANCTGNN
              901 CTGANTACNN NNTGAANNNC CTCACCGANC GCNNNTACNG CTNCACCNCN NNNGNCNANC
              961 GGGAAATCNN NGNNTGNNAT TAANGANAAN NCTGTGCNNN NGTCNNCCNN GNNCTNNNNN
            1021 NCANNNNNNN NGNNCNNNNN TGNNTNNCAG CNNCNTNCCN NNGNANNAAN NNNNNNNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB18314_B0H4d.seq check
            >D07039I2_B0H4__2_SV40pro_ori_F_C06_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNGNC GCCTCNGCCT CTGNNCTATT CCNGAAGTAG TGAGGAGGCT
               61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
              121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
              181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
              241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
              301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
              361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
              421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
              481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
              541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
              601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
              661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
              721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCAGGACA
              781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGNGCT GANCGCTTCC
              841 TCGNGCTTTA CGGTATCGCC GCTCCNGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG
              901 ANGAGTNCTT CTGANCGGGA NTCTGGGGTT CGAAATGANC GACCNAGCGA CGCCCAACCT
              961 GNCNNCACGA NNNTTCGATN NCNNCGNCNN CCNTCTATGA AANGNNGGGN TNNNNAANNN
            1021 NTTTNNCNGG NNNNNNNNNC NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

            2023.10.06

            GNP_filter3_RDBDEP_html_231001.pl