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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) Mitochondria-targeted mKeima-Red (pMT-mKeima-Red) (#RDB18321)

Distribution of this resource is pending.

Alternative name AM-V0251M
Clone info. ・For mammalian expression of the Mitochondria-targeted fluorescent protein.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone pMT (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18321zzk01.seq provided by the depositor
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Availability
RDB18321 CoralHue(TM) Mitochondria-targeted mKeima-Red (pMT-mKeima-Red) Distribution of this resource is pending.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) Mitochondria-targeted mKeima-Red (pMT-mKeima-Red) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18321).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18321_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'(MT Targeted mKeima-Red 5')
Sequence file: RDB18321_B0H4a.seq check
>18321_18321_B1KgF_CMV-Forward_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNCGTGTACG GTGGGNGGTC
   61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCATTGCCA CCATGCTGAG
  121 CCTGCGCCAG AGTATCCGCT TCTTCAAGCC CGCCACCAGG ACTCTGTGCA GTTCCAGGGC
  181 GGCCGCGGGG ACAATGGTGA GTGTGATCGC TAAACAAATG ACCTACAAGG TTTATATGTC
  241 AGGCACGGTC AATGGACACT ACTTTGAGGT CGAAGGCGAT GGAAAAGGAA AGCCTTACGA
  301 GGGAGAGCAG ACAGTAAAGC TCACTGTCAC CAAGGGTGGA CCTCTGCCAT TTGCTTGGGA
  361 TATTTTATCA CCACAGCTTC AGTACGGAAG CATACCATTC ACCAAGTACC CTGAAGACAT
  421 CCCTGATTAT TTCAAGCAGT CATTCCCTGA GGGATATACA TGGGAGAGGA GCATGAACTT
  481 TGAAGATGGT GCAGTGTGTA CTGTCAGCAA TGATTCCAGC ATCCAAGGCA ACTGTTTCAT
  541 CTACAATGTC AAAATCTCTG GTGAGAACTT TCCTCCCAAT GGACCTGTTA TGCAGAAGAA
  601 GACACAGGGC TGGGAACCCA GCACTGAGCG TCTCTTTGCA CGAGATGGAA TGCTGATAGG
  661 AAACGATTAT ATGGCTCTGA AGTTGGAAGG AGGTGGTCAC TATTTGTGTG AATTTAAATC
  721 TACTTACAAG GCANAGAAGC CTGTGANGAT GCCAGGGCGC CACGAGATTG ACCGCNAACT
  781 GGATGTAACC AGTCACAACA GGGATTACAC ATCTGTTGAG CAGTGTGAAA TAGCCATTGC
  841 ACGCCACTCT TNGCTCGGNT AANCTAGANG GGGGCCGCNA NTCTNGATCA TAATCAGCCN
  901 TACCNNATTT GNAGANGTTT TACTTGCTTT NAAAANNNNN NCNNCANCNT NCCNCNNNNA
961 NNNNNNANNN NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'(MT Targeted mKeima-Red 3')
Sequence file: RDB18321_B0H4b.seq check
>D07039I9_B0H4_4_SV40polyA-R2_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNAANNT TAANNACNAC NATTGCATTC ATTTTATGTT
   61 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
  121 TATGGCTGAT TATGATCTAG AGTCGCGGCC CCCTCTAGAT TAACCGAGCA AAGAGTGGCG
  181 TGCAATGGCT ATTTCACACT GCTCAACAGA TGTGTAATCC CTGTTGTGAC TGGTTACATC
  241 CAGTTTGCGG TCAATCTCGT GGCGCCCTGG CATCCTCACA GGCTTCTTTG CCTTGTAAGT
  301 AGATTTAAAT TCACACAAAT AGTGACCACC TCCTTCCAAC TTCAGAGCCA TATAATCGTT
  361 TCCTATCAGC ATTCCATCTC GTGCAAAGAG ACGCTCAGTG CTGGGTTCCC AGCCCTGTGT
  421 CTTCTTCTGC ATAACAGGTC CATTGGGAGG AAAGTTCTCA CCAGAGATTT TGACATTGTA
  481 GATGAAACAG TTGCCTTGGA TGCTGGAATC ATTGCTGACA GTACACACTG CACCATCTTC
  541 AAAGTTCATG CTCCTCTCCC ATGTATATCC CTCAGGGAAT GACTGCTTGA AATAATCAGG
  601 GATGTCTTCA GGGTACTTGG TGAATGGTAT GCTTCCGTAC TGAAGCTGTG GTGATAAAAT
  661 ATCCCAAGCA AATGGCAGAG GTCCACCCTT GGTGACAGTG AGCTTTACTG TCTGCTCTCC
  721 CTCGTAAGGC TTTCCTTTTC CATCGCCTTC GACCTCAAAG TAGTGTCCAT TGACCGTGCC
  781 TGACATATAA ACCTTGTANG TCATTTGTTT AGCGATCACA CTCACCATTG TCCCCGCGGC
  841 CGCCCTGNAA CTGCACAGAN TCCTGGTGGC GGGCTTGAAG AAGCGNANAC TCTGGCGCNN
  901 NTCNNCATGG NGGCNANGCT AGCGGATCTN ANGGTNCACN NAACCAGCTC TGCTNATATA
961 NANNTCCCNC NNNACACGCC NANCNNCNNT TTGCGTCNAN GGGGNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB18321_B0H4c.seq check
>D07039I9_B0H4_4_SV40pro_ori_F_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNGNNGNCN CCTCNNCCTC TGAGCTATTC CNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCA TCAAGAGACA GGATGAGGAT CGTTTCGCAT
  121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
  181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
  241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
  301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
  361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
  421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
  481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
  541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
  601 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG
  661 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG
  721 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT
  781 ANCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGNCGGC GAATGNGCTG ANCGCTTCCT
  841 CGNGCTTTAC NGTATCGCCG CNCCCNATTC NCAGCGCATC GCCTTCTATC GCCTTCTTGA
  901 NNANTTNTTC TGANCGGGAN TCNNGGGGTT CGAAATGACC GANCNANCGA CGCCCNNCNN
961 GNCNTCNNNA GATTTCNNNT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]