Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK265

Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, hygromycin resistance.

Sequence (full)	RDB18345zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18345_B2Hap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739) Region: insert 5'(SP6 pro,homology arm.1,mAID,mClover 5') Sequence file: RDB18345_B2Haa.seq
>D07105D6_B2Ha_1_pUC-CAPbs_up_F2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTTNNNNCT TNATGCTTCC GGCTCGTATG TTGTGTGGAA    61 TTGTGAGCNN ANNNNNNNTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCNNNCTAT   121 TTAGGTGACA CTATAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA   181 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTGCTA AAACTGGAGC TGAATCTATC   241 AGTTTGCTTG AGTTATGTCG AAATACGAAC AGAAAACAAG CTGCCGCAAA GTTCTACAGC   301 TTCTTGGTTC TTAAAAAGCA GCAAGCTATT GAGCTGACAC AGGAAGAACC GTACAGTGAC   361 ATCATCGCAA CACCTGGACC AAGGTTCCAT ATTATAGGAT CCGGTGCAGG CGCCAAGGAG   421 AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA AGGCACAAGT TGTGGGATGG   481 CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT GCCAAAAATC AAGCGGTGGC   541 CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG CACCGTACTT GAGGAAAATC   601 GATTTGAGGA TGTATAAAGC TAGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG   661 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTCCGCGGC   721 GAGGGCGAGG GCGATGCCAC CAACGGCAAG CTGANCCTGA AGTTCATCTG CACCACCGGC   781 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG NNCACCTTCG GCTACNGCGT GGCCTGCTTC   841 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCN AGTNCGCCAN GCCCGAANGC   901 TACNNNCAGG ANCGCANCAT CTCTTTCNNG GANNANNGNA CCTANNNGAN CCGCGCCNAN   961 GTGAAGTNCN AGGGCNANNC CCNNNNNNNN NGCATCNNNC NNNNNNNATC NNNNTNNNNN 1021 NNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: T7 pro,insert 3'(homology arm.2,T3 pro,loxP.2,mPGK ter) Sequence file: RDB18345_B2Hab.seq
>D07105D6_B2Ha_1_pUC-M13_-100_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCN NGGGTTTTCC CNGTCNCGAC GTTGTAAAAC    61 GACGGCCAGT GNNNNNNNNN NTACGACTCA CTATAGGGCG AATTGGGCCC TCTAGATGCA   121 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CGCCCTTTCC CTCAAAGATG   181 AAATTGACAA ATTTAATGTA CTGGAAAAAA ATGAAGAAGG AAAAAGGCAA AGACTTTGTA   241 CAGACAAAAA TCTAAGTTTT CTCAAAGGGT TCTGTGTCCC CTACACATGG GGGCAATTTG   301 TAAGCACTAG TGAATCAAAC ACTAGCTATA ATGCTTCTAG CTGCGCGCAA TTAACCCTCA   361 CTAAAGGGAA CAAAAGCTGG GTACCGGATC CTAGTGAACC TCTTCGAGGG ACCTAATAAC   421 TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC CGGATCTCGA CCAGCTTCTG   481 ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT GTATGTGGAA CAGAGGCTGC   541 TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA ACCACAGTAC TTAGAACCAC   601 AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA TCTATAGATC TATAGATCAT   661 GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT GTTTAAATTA TGATATCCAA   721 CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA GTAAAGAGCA NGCATTTATC   781 TAATCCCACC CCACCCCCAC CCCCGTAGCT CCNATCCTTC CATTCAAAAT GTANGTACTC   841 TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT CNATTTNAGT GGANATCTTT   901 ATTGTTTAAT AGATCATCAA TTTCTGCNTC CCNGGGNTCT GATATCATCN ATGCNTGGGG   961 GTCNNGCNCT CCTTTCGGNC GGNNNCTGNN GGNNNNGGGG CGGGNGGCNA NNCNTATTNC 1021 NNNNNCNNNN NNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert mid(HygR) Sequence file: RDB18345_B2Hac.seq
>D07105D6_B2Ha_1_PGKpro_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNN NNNNTCCNNG NCTTTCGACC TGCATCCNTC TAGATCTCGA    61 GCAGCTGANN NNNNNNNCTA GCATGGNTAG ATCCGGAAAG CCTGAACTCA CCNNGACGTC   121 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA   181 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT   241 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC   301 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG   361 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC   421 TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC   481 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT   541 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT   601 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA   661 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG   721 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GANTCCCAAT ACGAGGTCGC   781 CAACATCTTC TTCTGGANGC CGTGGTTGGC TTGTATGGAG CAGCAGANGC GCTACTTCGA   841 NCGGANGCAT CCGGANCTTG CNNGANCGCC GCGGCTCCNG GCGTATATGC TCCGCATTGG   901 TCTTGACCAA CTCTATCAGA NCTNNNNTNG ANGGCNATTT CGATGANGCA NCTTGGGNNC   961 NNGNNCGANG CNNNGCNNNC NNNCNATNNN GNANNCNNGN NNNGNNGGNN NNNNNNNANN 1021 NNNNNGNNNA NNNNNNNCNN NNNNNNNN //
Primer: PGKpro_R (Pr0793) Region: insert mid(PGK pro,loxP.1,SV40 pA,mClover3') Sequence file: RDB18345_B2Had.seq
>D07105D6_B2Ha_1_PGKpro_R_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGNGTGCG ANGCCNGANG CCACTTGTGT AGCGCCNAGT    61 GCCCAGCGNN NNNNNNNNAG CGCATGCTCC AGACTGCCTT GGGAAAAGCG CCNCNNCTAC   121 CCGGTAGAAT TTCGAGGTCG AGATCCTAAG CTTGGCTGGA CGTAAACTCC TCTTCAGACC   181 TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTATTG AATATGATCG   241 ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT   301 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA   361 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG   421 AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCAGTTAT   481 CTAGATTACT TGTACAGCTC GTCCATGCCA TGTGTAATCC CGGCGGCGGT CACGAACTCC   541 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGATGGCTC   601 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG   661 TGGTCGGCGA GCTGCACGCT GCCGTCCTCA ACGTTGTGGC GGATCTTGAA GTTAGCCTTG   721 ATGCCGTTCT TCTGCTTGTC GGCCGTGATA TAGACGTTGT GGCTGTTGAA GTTGTACTCC   781 AGCTTGTGCC CCANGNTGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG   841 TTCACCAGGG TGTCGCCNNC GAACTTCACC TCNGCGCGGG TCTTGTANGT ACCGTCGTNC   901 TTNAAAGAGA NNGTGCGCTC CNNGNNGTAN CNNTCNGGCA TGGNGGANTT GAAGAANTCG   961 NGCTGCTTNN TGTNNNCNGG NNNNCGGCTG NNNCNNNNNC NNNNNNNCNN NNNNNNGNNN 1021 NNNNNGNNNG NNNNNNNNNN //
Primer: EGFP-M_R (Pr0743) Region: insert mid(mClover 3',mAID,homology arm.1) Sequence file: RDB18345_B2Hae.seq
>D07105D6_B2Ha_1_EGFP-M_R_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNTNGTGNN GGNNCTTGAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG    61 GCNNNNNNNT ANACGTTGTG GCTGTTGAAG TTGTACTCCA GCTTGNGCCC CAGGATGTTG   121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG   181 AACTTCACCT CGGCGCGGGT CTTGTAGGTA CCGTCGTCCT TGAAAGAGAT GGTGCGCTCC   241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG   301 TAGCGGCTGA AGCAGGCCAC GCCGTAGCCG AAGGTGGTCA CGAGGGTGGG CCAGGGCACG   361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTTGGT GGCATCGCCC   421 TCGCCCTCGC CGCGGACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG   481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGCTAGC TTTATACATC   541 CTCAAATCGA TTTTCCTCAA GTACGGTGCT CCGTCCATTG ATACCTTCAC GAACGCCGCC   601 GCCTCCGGGC CACCGCTTGA TTTTTGGCAG GAAACCATCA CGTTCTTCCG GTATGATCTC   661 ACCGGTGGCC ATCCCACAAC TTGTGCCTTG GCCGGAGGTT TGGCTGGATC TTTAGGACAA   721 GCACTCTTCT CCTTGGCGCC TGCACCGGAT CCTATAATAT GGAACCTTGG TCCAGGTGTT   781 GCGATGATGT CACTGTACGG TTCTTCCTGT GTCAGCTCAA TAGCTTGCTG CTTTTTAAGA   841 ACCAAGAAGC TGTANAACTT TGCGGCAGCT TGTTTTCTGT TCGTATTTCN ACATANCTCA   901 AGCAAACNGA TAGANNCAGC TCCNGTTTTA GCNAGGNCGA ATTNCNGCAC ACNGGNCGNN 961 CGTNACTANN NGNTNCNNNC NCNGNACNNN GCNNNNNNNN NN //
Primer: Zeo-M_R (Pr0675) Region: BleoR,KanR Sequence file: RDB18345_B2Haf.seq
>D07105D6_B2Ha_1_Zeo-M_R_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNNNNNNNCC CNGNNNNNNN TGTTGTCCGG    61 CACCACCTGG TCCNGGNNNN NGCTGATGAA CAGGGTCACG TCGTCCCGGA CCACACCGGC   121 GAAGTCGTCC TCCACGAAGT CCCGGGAGAA CCCGAGCCGG TCGGTCCAGA ACTCGACCGC   181 TCCGGCGACG TCGCGCGCGG TGAGCACCGG AACGGCACTG GTCAACTTGG CCATGGTGGC   241 CCTCCTCACG TGCTATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT   301 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT   361 GCCACCTGAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGA   421 AATTGTAAGC GTTAATAATT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT   481 GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA   541 GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGGTCC GCCACACCCA   601 GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC   661 AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG   721 CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA   781 GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG   841 NNNANGNAGC CGGATCANGC GTATGCAGCC GCCNCATTGC ATCAGCCATG ATGGATACTT   901 TCTCGGCANG ANCAAGGNNA NATGANNNNA NATNCNGNCC CCNGNNCTTC NCCNNNNANC 961 NNNCANNNCN NNCCNGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl