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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK265 (#RDB18345)

Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, hygromycin resistance.

Alternative name RAD21-mAC Hygro
Clone info. Donor plasmid to generate RAD21-mAID degron cell lines with mClover as a marker, hygromycin resistance, see Fig. 3 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Kanamycin (E. coli), Hygromycin (mammalian cell).
Gene/insert name human RAD21 genomic Arabidopsis thaliana IAA17 cDNA Aequorea victoria GFP (mClover) cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18345zzk01.seq provided by the depositor

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18345 pMK265 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK265 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18345).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18345_B2Hap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: insert 5'(SP6 pro,homology arm.1,mAID,mClover 5')
Sequence file: RDB18345_B2Haa.seq check
>D07105D6_B2Ha_1_pUC-CAPbs_up_F2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTTNNNNCT TNATGCTTCC GGCTCGTATG TTGTGTGGAA
   61 TTGTGAGCNN ANNNNNNNTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCNNNCTAT
  121 TTAGGTGACA CTATAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
  181 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTGCTA AAACTGGAGC TGAATCTATC
  241 AGTTTGCTTG AGTTATGTCG AAATACGAAC AGAAAACAAG CTGCCGCAAA GTTCTACAGC
  301 TTCTTGGTTC TTAAAAAGCA GCAAGCTATT GAGCTGACAC AGGAAGAACC GTACAGTGAC
  361 ATCATCGCAA CACCTGGACC AAGGTTCCAT ATTATAGGAT CCGGTGCAGG CGCCAAGGAG
  421 AAGAGTGCTT GTCCTAAAGA TCCAGCCAAA CCTCCGGCCA AGGCACAAGT TGTGGGATGG
  481 CCACCGGTGA GATCATACCG GAAGAACGTG ATGGTTTCCT GCCAAAAATC AAGCGGTGGC
  541 CCGGAGGCGG CGGCGTTCGT GAAGGTATCA ATGGACGGAG CACCGTACTT GAGGAAAATC
  601 GATTTGAGGA TGTATAAAGC TAGCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
  661 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTCCGCGGC
  721 GAGGGCGAGG GCGATGCCAC CAACGGCAAG CTGANCCTGA AGTTCATCTG CACCACCGGC
  781 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG NNCACCTTCG GCTACNGCGT GGCCTGCTTC
  841 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCN AGTNCGCCAN GCCCGAANGC
  901 TACNNNCAGG ANCGCANCAT CTCTTTCNNG GANNANNGNA CCTANNNGAN CCGCGCCNAN
  961 GTGAAGTNCN AGGGCNANNC CCNNNNNNNN NGCATCNNNC NNNNNNNATC NNNNTNNNNN
1021 NNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 3'(homology arm.2,T3 pro,loxP.2,mPGK ter)
Sequence file: RDB18345_B2Hab.seq check
>D07105D6_B2Ha_1_pUC-M13_-100_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGNNNGCN NGGGTTTTCC CNGTCNCGAC GTTGTAAAAC
   61 GACGGCCAGT GNNNNNNNNN NTACGACTCA CTATAGGGCG AATTGGGCCC TCTAGATGCA
  121 TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CGCCCTTTCC CTCAAAGATG
  181 AAATTGACAA ATTTAATGTA CTGGAAAAAA ATGAAGAAGG AAAAAGGCAA AGACTTTGTA
  241 CAGACAAAAA TCTAAGTTTT CTCAAAGGGT TCTGTGTCCC CTACACATGG GGGCAATTTG
  301 TAAGCACTAG TGAATCAAAC ACTAGCTATA ATGCTTCTAG CTGCGCGCAA TTAACCCTCA
  361 CTAAAGGGAA CAAAAGCTGG GTACCGGATC CTAGTGAACC TCTTCGAGGG ACCTAATAAC
  421 TTCGTATAGC ATACATTATA CGAAGTTATA TTAAGGGTTC CGGATCTCGA CCAGCTTCTG
  481 ATGGAATTAG AACTTGGCAA AACAATACTG AGAATGAAGT GTATGTGGAA CAGAGGCTGC
  541 TGATCTCGTT CTTCAGGCTA TGAAACTGAC ACATTTGGAA ACCACAGTAC TTAGAACCAC
  601 AAAGTGGGAA TCAAGAGAAA AACAATGATC CCACGAGAGA TCTATAGATC TATAGATCAT
  661 GAGTGGGAGG AATGAGCTGG CCCTTAATTT GGTTTTGCTT GTTTAAATTA TGATATCCAA
  721 CTATGAAACA TTATCATAAA GCAATAGTAA AGAGCCTTCA GTAAAGAGCA NGCATTTATC
  781 TAATCCCACC CCACCCCCAC CCCCGTAGCT CCNATCCTTC CATTCAAAAT GTANGTACTC
  841 TGTTCTCACC CTTCTTAACA AAGTATGACA GGAAAAACTT CNATTTNAGT GGANATCTTT
  901 ATTGTTTAAT AGATCATCAA TTTCTGCNTC CCNGGGNTCT GATATCATCN ATGCNTGGGG
  961 GTCNNGCNCT CCTTTCGGNC GGNNNCTGNN GGNNNNGGGG CGGGNGGCNA NNCNTATTNC
1021 NNNNNCNNNN NNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: insert mid(HygR)
Sequence file: RDB18345_B2Hac.seq check
>D07105D6_B2Ha_1_PGKpro_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN NNNNTCCNNG NCTTTCGACC TGCATCCNTC TAGATCTCGA
   61 GCAGCTGANN NNNNNNNCTA GCATGGNTAG ATCCGGAAAG CCTGAACTCA CCNNGACGTC
  121 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA
  181 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT
  241 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC
  301 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG
  361 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC
  421 TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC
  481 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT
  541 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT
  601 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA
  661 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG
  721 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GANTCCCAAT ACGAGGTCGC
  781 CAACATCTTC TTCTGGANGC CGTGGTTGGC TTGTATGGAG CAGCAGANGC GCTACTTCGA
  841 NCGGANGCAT CCGGANCTTG CNNGANCGCC GCGGCTCCNG GCGTATATGC TCCGCATTGG
  901 TCTTGACCAA CTCTATCAGA NCTNNNNTNG ANGGCNATTT CGATGANGCA NCTTGGGNNC
  961 NNGNNCGANG CNNNGCNNNC NNNCNATNNN GNANNCNNGN NNNGNNGGNN NNNNNNNANN
1021 NNNNNGNNNA NNNNNNNCNN NNNNNNNN
//
Primer: PGKpro_R (Pr0793)
Region: insert mid(PGK pro,loxP.1,SV40 pA,mClover3')
Sequence file: RDB18345_B2Had.seq check
>D07105D6_B2Ha_1_PGKpro_R_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGNGTGCG ANGCCNGANG CCACTTGTGT AGCGCCNAGT
   61 GCCCAGCGNN NNNNNNNNAG CGCATGCTCC AGACTGCCTT GGGAAAAGCG CCNCNNCTAC
  121 CCGGTAGAAT TTCGAGGTCG AGATCCTAAG CTTGGCTGGA CGTAAACTCC TCTTCAGACC
  181 TAATAACTTC GTATAGCATA CATTATACGA AGTTATATTA AGGGTTATTG AATATGATCG
  241 ACGCGTTAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT
  301 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA
  361 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG
  421 AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCAGTTAT
  481 CTAGATTACT TGTACAGCTC GTCCATGCCA TGTGTAATCC CGGCGGCGGT CACGAACTCC
  541 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCTTGGA CTGATGGCTC
  601 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
  661 TGGTCGGCGA GCTGCACGCT GCCGTCCTCA ACGTTGTGGC GGATCTTGAA GTTAGCCTTG
  721 ATGCCGTTCT TCTGCTTGTC GGCCGTGATA TAGACGTTGT GGCTGTTGAA GTTGTACTCC
  781 AGCTTGTGCC CCANGNTGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG
  841 TTCACCAGGG TGTCGCCNNC GAACTTCACC TCNGCGCGGG TCTTGTANGT ACCGTCGTNC
  901 TTNAAAGAGA NNGTGCGCTC CNNGNNGTAN CNNTCNGGCA TGGNGGANTT GAAGAANTCG
  961 NGCTGCTTNN TGTNNNCNGG NNNNCGGCTG NNNCNNNNNC NNNNNNNCNN NNNNNNGNNN
1021 NNNNNGNNNG NNNNNNNNNN
//
Primer: EGFP-M_R (Pr0743)
Region: insert mid(mClover 3',mAID,homology arm.1)
Sequence file: RDB18345_B2Hae.seq check
>D07105D6_B2Ha_1_EGFP-M_R_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNTNGTGNN GGNNCTTGAG TTAGCCTTGA TGCCGTTCTT CTGCTTGTCG
   61 GCNNNNNNNT ANACGTTGTG GCTGTTGAAG TTGTACTCCA GCTTGNGCCC CAGGATGTTG
  121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG
  181 AACTTCACCT CGGCGCGGGT CTTGTAGGTA CCGTCGTCCT TGAAAGAGAT GGTGCGCTCC
  241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG
  301 TAGCGGCTGA AGCAGGCCAC GCCGTAGCCG AAGGTGGTCA CGAGGGTGGG CCAGGGCACG
  361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTTGGT GGCATCGCCC
  421 TCGCCCTCGC CGCGGACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG
  481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGCTAGC TTTATACATC
  541 CTCAAATCGA TTTTCCTCAA GTACGGTGCT CCGTCCATTG ATACCTTCAC GAACGCCGCC
  601 GCCTCCGGGC CACCGCTTGA TTTTTGGCAG GAAACCATCA CGTTCTTCCG GTATGATCTC
  661 ACCGGTGGCC ATCCCACAAC TTGTGCCTTG GCCGGAGGTT TGGCTGGATC TTTAGGACAA
  721 GCACTCTTCT CCTTGGCGCC TGCACCGGAT CCTATAATAT GGAACCTTGG TCCAGGTGTT
  781 GCGATGATGT CACTGTACGG TTCTTCCTGT GTCAGCTCAA TAGCTTGCTG CTTTTTAAGA
  841 ACCAAGAAGC TGTANAACTT TGCGGCAGCT TGTTTTCTGT TCGTATTTCN ACATANCTCA
  901 AGCAAACNGA TAGANNCAGC TCCNGTTTTA GCNAGGNCGA ATTNCNGCAC ACNGGNCGNN
961 CGTNACTANN NGNTNCNNNC NCNGNACNNN GCNNNNNNNN NN
//
Primer: Zeo-M_R (Pr0675)
Region: BleoR,KanR
Sequence file: RDB18345_B2Haf.seq check
>D07105D6_B2Ha_1_Zeo-M_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNNNNNNNCC CNGNNNNNNN TGTTGTCCGG
   61 CACCACCTGG TCCNGGNNNN NGCTGATGAA CAGGGTCACG TCGTCCCGGA CCACACCGGC
  121 GAAGTCGTCC TCCACGAAGT CCCGGGAGAA CCCGAGCCGG TCGGTCCAGA ACTCGACCGC
  181 TCCGGCGACG TCGCGCGCGG TGAGCACCGG AACGGCACTG GTCAACTTGG CCATGGTGGC
  241 CCTCCTCACG TGCTATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
  301 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
  361 GCCACCTGAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGA
  421 AATTGTAAGC GTTAATAATT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT
  481 GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA
  541 GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGGTCC GCCACACCCA
  601 GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC
  661 AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG
  721 CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA
  781 GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG
  841 NNNANGNAGC CGGATCANGC GTATGCAGCC GCCNCATTGC ATCAGCCATG ATGGATACTT
  901 TCTCGGCANG ANCAAGGNNA NATGANNNNA NATNCNGNCC CCNGNNCTTC NCCNNNNANC
961 NNNCANNNCN NNCCNGNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]