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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

RAD21 C-tag CIRPSR (#RDB18346)

Expression vector of Cas9 and gRNA for a RAD21 tagging donor.

Clone info. Expression vector of Cas9 and gRNA for a RAD21 tagging donor, see Fig. 3 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone px330 (plasmid)
Selectable markers Ampicillin
Gene/insert name human RAD21 cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18346zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18346 RAD21 C-tag CIRPSR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The RAD21 C-tag CIRPSR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18346).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18346_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3element Reverse (Pr0108)
Region: U6 pro,sgRNA for RAD21,CBh pro
Sequence file: RDB18346_B0Gfa.seq check
>D07105B5_B0Gf_4_SV40pA3'element Reverse_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNG CNNGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTNNNNN NNNNNTTGCT CACATGTGAG GGCCTATTTC CCATGATTCC TTCATATTTG
  121 CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG
  181 ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA
  241 AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
  301 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGCCA AGGTTCCATA TTATATAGTT
  361 TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC
  421 ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
  481 GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC
  541 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG
  601 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC
  661 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG
  721 GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC
  781 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA NGTGAGCCCC ACGTTCTGCT
  841 TCACTCTCCC CATCTCCCCC CCCCNCNCCA NCCCCAATTT TGTATTTATT TATTTTTTNN
901 ATTATTTTGT GCANCGATGG GGGCNNNNNN NNNN
//
Primer: b-actin pro_F1 (Pr1046)
Region: 3xFLAG,SV40NLS,Cas9
Sequence file: RDB18346_B0Gfb.seq check
>D07105B5_B0Gf_4_b-actin pro_F1_C06_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGTGGGGNN NTAATGTTTA ATTACCTGGN
   61 NNNNNNGCCT GAANTCNNTT TTTTTCNGGT TGGACCGGTG NCNCCATGGA CTATAAGGAC
  121 CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC
  181 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC
  241 ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG
  301 GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC
  361 CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA
  421 ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC
  481 AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG
  541 GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG
  601 GCCTACCACG AGAAGTACCC CACCATCTAN NNNNNGAGAA AGAAACTGGT GGACAGCACC
  661 GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGNNNN NNATGATCAA GTTCCGGGGC
  721 CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACANNN NNNTGGACNA AGCTGTTCNT
  781 CCAGCTGGTG NANANCTACA NCCNGCTGTT CGAGGNAAAA CCCCATCNAN NNCCAGCGGC
  841 GTGGNACGCC NNNNGCCATC NNNNTCTGCC AGACTGANNN ANNANCANAN NGGCNGGAAA
  901 ATNNNNNNNC CCANNTGNCC NGCNNNGNAN AANANNGGNN NNNGTNNNGN ANNNNNNNNN
961 N
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,nucleoplasmin NLS,Cas9
Sequence file: RDB18346_B0Gfc.seq check
>D07105B5_B0Gf_4_bGH_rev3_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGNNAAACAA CNGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC
  121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG
  181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC
  241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA
  301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC
  361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT
  421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA
  481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA
  541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC
  601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA
  661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG
  721 GATTCTTCTC NAAGCTGCTT CTTTCCATGA TGGTGATCCC CANCAGCTCT TTCACACTCT
  781 TCAGTTTCTT GGACTTGCCC TTTTNCACTT TGGCCNCCAC CAGCACANNN NNNGNCACNG
  841 TGGGGCTGTC GAANCCGCCN TACTTCTTNN GNNCCCNNNN CTTCNTTCTG GNNANCAGCT
  901 TATCNNTGNT NNCTCTTGGG CNNGANANAN TCTTTNNCTG ANNCCGCCNG TCNGCNNCTC
961 GNNCNTTTNN ACNNNNNTCN CNTGGGNNAN GNTCNNNNNN TNCNNNNNNN NNN
//
Primer: Cas9-N_R1 (Pr0650)
Region: SV40 NLS,3xFLAG,CBh pro
Sequence file: RDB18346_B0Gfd.seq check
>D07105B5_B0Gf_4_Cas9-N_R1_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTGNNNT CGTCGGTGNN CACGGCCCAG CCCACAGAGT
   61 TGGTGCNNNN NNNNNNNNNN ATGCTGTACT TCTTGTCGGC TGCTGGNACT NNNTGGATAC
  121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT
  181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA
  241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA
  301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG
361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGN GNGAGGNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]