Please review the QC test results indicated by icon below as well as clone information before placing your order.

CTCF-mAC donor (Hygro)

Donor plasmid to generate CTCF-mAID degron cell lines with mClover as a marker, hygromycin resistance.

Sequence (full)	RDB18354zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18354_B2Hap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-M_R (Pr0743) Region: insert mid(mClover 5',mAID 3') Sequence file: RDB18354_B2Haa.seq
>D07105C4_B2Ha_3_EGFP-M_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNANNTNNTG NNGGNNCTNN NNNTAGCCTT GATGCCGTTC TTCTGCTTGT    61 CGGNNNNNNN NTANACGTTG TGGCTGTTGA AGTTGTACTC CAGCTTGTGC CCCAGGATGT   121 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT   181 CGAACTTCAC CTCGGCGCGG GTCTTGTAGG TACCGTCGTC CTTGAAAGAG ATGGTGCGCT   241 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG   301 GGTAGCGGCT GAAGCAGGCC ACGCCGTAGC CGAAGGTGGT CACGAGGGTG GGCCAGGGCA   361 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTTG GTGGCATCGC   421 CCTCGCCCTC GCCGCGGACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA   481 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGCTA GCTTTATACA   541 TCCTCAAATC GATTTTCCTC AAGTACGGTG CTCCGTCCAT TGATACCTTC ACGAACGCCG   601 CCGCCTCCGG GCCACCGCTT GATTTTTGGC AGGAAACCAT CACGTTCTTC CGGTATGATC   661 TCACCGGTGG CCATCCCACA ACTTGTGCCT TGGCCGGAGG TTTGGCTGGA TCTTTANGAC   721 AAGCACTCTT CTCCTTGGCG CCTGCACCGG ATCCCCGGTC CATCATGCTG ANGATCATCT   781 CGGGCGTGAN GTCTCCGTTG GGGGCATCTG TGGCAGCTGG CTGGGCCTCC TCTTCCTCTC   841 NCTCTGCNGG CTCAGCATCT GGCTCTTTTT TTTACTTCAA CTATAATGNT CTCNATTGCA   901 CCNNTATTCT GGNCTTCAAC CNGNANTGAT AGCTGTTGCT GGNAAANAAN ANCNCAGANA 961 GAACAAATGG NNCATNNAAG CCCNNNGAAT CANNNNNNNN NTTNNNNNNN NNN //
Primer: mPGKter_F (Pr0894) Region: insert 3'(loxP.2,CTCF homology arm.2) Sequence file: RDB18354_B2Hab.seq
>D07105C4_B2Ha_3_mPGKter_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT GTTTTNCNNA GTTCTAATTC NNTCAGAAGC TGGTCGAGAT    61 CCGGAACCCT NNNNNNNNNC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCCCTCG   121 AAGAGGTTCA CTAGGATCCG CGTCGCCAGG ACTTCTCTGG GCTGTGTTTA AACGGCCCGC   181 ATCTTAATTT TTCTCCCTTC TTTCTTTTTT TGGCTTTGGG AAAAGCATCA TTTTACCAAA   241 CATACCGAGA ACGAAAACTT CAAGGATGAT GTTAGAAAAA AATGTGATTT AACTAGAACT   301 TGCTGTCTGA TGTTAGCAAA TCATGGAATG TTCTGAGTCC CTGAGGGTTT ACTGTGAAGT   361 GCTGAGGACA GTGTTGACAA CTAACTCGTT TTCCTAGATG GAAACGGAGA CATTGACCCC   421 TCCCTCCATG TGGTAAACCA CTCCAGAATG GCCACCAGGC TTCCCAGAGT TCTATGGTCT   481 TCTTCCCAAG AGAGTTTTTA ATTGTAAATG CATACTTGGG AAGGACTTAG AGTTTTAAAC   541 TGTTTTTTGC TTTTGCTTTT CCCTGACTCC CTTTGCTTGG AGGGTACCCA GCTTTTGTTC   601 CCTTTAGTGA GGGTTAATTG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG   661 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGGAGCATAA AGTGTAAAGC   721 CTGGNGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT   781 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGANAGG   841 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GANTCGCTGC GCTCGGNCGT   901 TCGGCTGCNG CNANCGGTAT CAGCTCACTC NAAGGCGGNA ATACNNNTAT NCNCAGNNTC 961 NNNGGNNNAC NCNNNAAGNN NNTGNNANCN AANNNNCANC AAANNNNNNN NNN //
Primer: PGK_N_rev (Pr0243) Region: insert mid(SV40 pA,mClover 3') Sequence file: RDB18354_B2Hac.seq
>D07105C4_B2Ha_3_PGK_N_rev_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNANANTTC GAGGTCGAGN NNCCTAAGCT TGGCTGGACG    61 TAAACTCCTN NNCNNACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TNNNNNTAAG   121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA   181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA   241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG   301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCATG TGTAATCCCG   421 GCGGCGGTCA CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC   481 AGCTTGGACT GATGGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG   541 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCAAC GTTGTGGCGG   601 ATCTTGAAGT TAGCCTTGAT GCCGTTCTTC TGCTTGTCGG CCGTGATATA GACGTTGTGG   661 CTGTTGAAGT TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG   721 CCCTTCAGCT CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC   781 TTGTANGTAC CGTCGTCCTT GAAAGAGATG GTGCGCTCCT GGACGTANCC TTCGGGCATG   841 GCGGANTTGA ANAANTCGTG CTGCTTCATG TGGTCGGGGT ANCGGCTGAA GCNNNCCACG   901 CCGTANCCGA NNNNGTCACN ANGGTGGGNC AGGGNACGGG CAGCTTGNCN GTGGTGNNNA 961 NGANNTTCNN GNNCANCNTG NCNNNNGNNN NNTCNNNNNG CCNNNNNNNN NN //
Primer: PGKpro_F (Pr0249) Region: insert mid(HygR) Sequence file: RDB18354_B2Had.seq
>D07105C4_B2Ha_3_PGKpro_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTCTCCNGG CCTTTCGACC TGCATCCNTC TAGATCTCGA    61 GCAGCTGANN NNTNNNNCTA GCATGGATAG ATCCGGAAAG CCTGAACTCA CCGCGACGTC   121 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA   181 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT   241 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC   301 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG   361 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC   421 TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC   481 GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT   541 CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT   601 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA   661 AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG   721 CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGANGTCGC   781 CAACATCTTC TTCTGGANGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCNA   841 GCGGANGCAT CCGGANCTTG CANGATCGCC GCGGCTCCNG GCGTATATGC TCCGCANTGG   901 TCTTGANCNN CTCTATCAGA NCNNNGNTNG ANGGCNATTT CGATGANGCN NCTTGNNNNC 961 NNGNNCGATG CNANNCNNNC NNNNNNTNNN GNANNCNNGN NNNGNNGGGN NNNNNNNNN //
Primer: pUC-CAPbs_up_F2 (Pr0739) Region: insert 3'(CTCF homology arm.2, loxP.2,mPGK ter) Sequence file: RDB18354_B2Hae.seq
>D07105C4_B2Ha_3_pUC-CAPbs_up_F2_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCTTTNNNC TTTATGCTCC CGGCTCGTAT GTTGTGTGGA    61 ATTGTGAGCG GNNNNNAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCNAGCGC   121 GCAATTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCC TCCAAGCAAA GGGAGTCAGG   181 GAAAAGCAAA AGCAAAAAAC AGTTTAAAAC TCTAAGTCCT TCCCAAGTAT GCATTTACAA   241 TTAAAAACTC TCTTGGGAAG AAGACCATAG AACTCTGGGA AGCCTGGTGG CCATTCTGGA   301 GTGGTTTACC ACATGGAGGG AGGGGTCAAT GTCTCCGTTT CCATCTAGGA AAACGAGTTA   361 GTTGTCAACA CTGTCCTCAG CACTTCACAG TAAACCCTCA GGGACTCAGA ACATTCCATG   421 ATTTGCTAAC ATCAGACAGC AAGTTCTAGT TAAATCACAT TTTTTTCTAA CATCATCCTT   481 GAAGTTTTCG TTCTCGGTAT GTTTGGTAAA ATGATGCTTT TCCCAAAGCC AAAAAAAGAA   541 AGAAGGGAGA AAAATTAAGA TGCGGGCCGT TTAAACACAG CCCAGAGAAG TCCTGGCGAC   601 GCGGATCCTA GTGAACCTCT TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA   661 AGTTATATTA AGGGTTCCGG ATCTCGACCA GCTTCTGATG GAATTAGAAC TTGGCAAAAC   721 AATACTGAGA ATGAAGTGTA TGTGGAACAG ANGCTGCTGA TCTCGTTCTT CANGCTATGA   781 AACTGACACA TTTGGAAACC ACAGTACTTA GAACCACAAA GTGGGAATCA AGAGAAAAAC   841 AATGATCCCA CGAGAGATCT ATAGATCTAT ANATCATGAN TGGGANGAAT GAGCTGGNCC   901 TTNATTTGGT TTTNNTTGNT TAAATTATGA TATNCNACTA TGAAACATTA TCATAAAGNN   961 NTAGTAAAGA NCCTNCNNTN AANANNANGN ATTTNNCTNA NTCCNNCCCN NNCCCNNNNN 1021 NCNNNNNNNN NN //
Primer: pUC-M13_-100 (Pr0310) Region: insert 5'(T7 pro,CTCF homology arm.1,mAID 5') Sequence file: RDB18354_B2Haf.seq
>D07105C4_B2Ha_3_pUC-M13_-100_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNANGCNN GGGTTTTCCC NGTCNCGACG TTGTAAAACG    61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CTGAGGCAGG   121 AGAATTGCTT TAACCCGGGG GGCAGAGGTT GCAGTGAGCT GAGATCATGC CCGTGCACTC   181 CAGCCTGGTC ATAGAGCAAG ACTCCGTCTC AAAAAATAAA ATAAAAAATA AATTGACTGT   241 CTCTGGACCG CTATCTAATA AGGCTGTCCT GCATTGCTGA CATCCCGTTC GCTGTCAGTC   301 TAAAAGACCC TTGTGATTCT TGGGGCTTTA ATGGACCATT TGTTCTGTCT GTGCTCTTCT   361 TTGCCAGCAA CAGCTATCAT TCAGGTTGAA GACCAGAATA CAGGTGCAAT TGAGAACATT   421 ATAGTTGAAG TAAAAAAAGA GCCAGATGCT GAGCCCGCAG AGGGAGAGGA AGAGGAGGCC   481 CAGCCAGCTG CCACAGATGC CCCCAACGGA GACCTCACGC CCGAGATGAT CCTCAGCATG   541 ATGGACCGGG GATCCGGTGC AGGCGCCAAG GAGAAGAGTG CTTGTCCTAA AGATCCAGCC   601 AAACCTCCGG CCAAGGCACA AGTTGTGGGA TGGCCACCGG TGAGATCATA CCGGAAGAAC   661 GTGATGGTTT CCTGCCAAAA ATCAAGCGGT GGCCCGGANG CGGCGGCGTT CGTGAAGGTA   721 TCAATGGACG GAGCACCGTA CTTGANGAAA ATCGATTTGA GGANGTATAA AGCTAGCATG   781 GTGAGCAAGG NCGAGGANCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA NCTGGACNGC   841 GACGTAAACG GCCACAAGTT CAGCGTCCGC GGCGAGGGCG ANGGCGANGN CNCCNACGGC   901 AAGCTGANCC NGAAGTTCAT CTGCACCACC NGNNAGCTGC CCNNNCCCNN NNCCNNNCNN 961 CNNGNNCANC NTNCNNNNAN NNNNNNNCNN GNTTCANNCN GCTACCCNNN NNNNNNN //
Primer: YFP_R (Pr0127) Region: insert 5'(mClover 5',mAID,CTCF homology arm.1) Sequence file: RDB18354_B2Hag.seq
>D07105C4_B2Ha_3_YFP_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN GNNGCTNANT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA    61 CCAGGANGGN NACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG CTAGCTTTAT   121 ACATCCTCAA ATCGATTTTC CTCAAGTACG GTGCTCCGTC CATTGATACC TTCACGAACG   181 CCGCCGCCTC CGGGCCACCG CTTGATTTTT GGCAGGAAAC CATCACGTTC TTCCGGTATG   241 ATCTCACCGG TGGCCATCCC ACAACTTGTG CCTTGGCCGG AGGTTTGGCT GGATCTTTAG   301 GACAAGCACT CTTCTCCTTG GCGCCTGCAC CGGATCCCCG GTCCATCATG CTGAGGATCA   361 TCTCGGGCGT GAGGTCTCCG TTGGGGGCAT CTGTGGCAGC TGGCTGGGCC TCCTCTTCCT   421 CTCCCTCTGC GGGCTCAGCA TCTGGCTCTT TTTTTACTTC AACTATAATG TTCTCAATTG   481 CACCTGTATT CTGGTCTTCA ACCTGAATGA TAGCTGTTGC TGGCAAAGAA GAGCACAGAC   541 AGAACAAATG GTCCATTAAA GCCCCAAGAA TCACAAGGGT CTTTTAGACT GACAGCGAAC   601 GGGATGTCAG CAATGCAGGA CAGCCTTATT AGATAGCGGT CCAGAGACAG TCAATTTATT   661 TTTTATTTTA TTTTTTGAGA CGGAGTCTTG CTCTATGACC AGGCTGGAGT GCACGGGCAT   721 GATCTCAGCT CACTGCAACC TCTGCCCCCC GGGTTAAAGC AATTCTCCTG CCTCAGANCT   781 CCAATTCGCC CTATAGTGAG TCGTANTACG CGCGCTCACT GGCCGTCGTT TTACAACGTC   841 GTGACTGGGA AAACCCTGGC GTTNCCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG   901 CCNGCTGGCG TAATANCNAA NANGNCCGCA CCGATCGCCC NNCCNNNCAN TTGCNCANCC   961 TGAATGGNGA ANGGNNNNCN NCNNGNANCN GCNCNNTNAN GCGNNGNNNG GNNNNNNNGN 1021 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl