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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CTCF-C CRISPR (#RDB18355)

Expression vector of Cas9 and gRNA for a CTCF tagging donor.

Clone info. Expression vector of Cas9 and gRNA for a CTCF tagging donor, see Fig. 4E of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone px330 (plasmid)
Selectable markers Ampicillin
Gene/insert name human CTCF cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18355zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
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必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18355 CTCF-C CRISPR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CTCF-C CRISPR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18355).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18355_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3element Reverse (Pr0108)
Region: U6 pro,sgRNA for CTCF,CBh pro
Sequence file: RDB18355_B0Gfa.seq check
>D07105C5_B0Gf_3_SV40pA3'element Reverse_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNANGC NNGCAACGCG GCCTTTTTAN GGTTCCTGGC
   61 CTTTTNNNNN NNNNNNNCNN NCATGTGANG GCCTATTTCC CATGATNNCN NNNNNTTTGC
  121 ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA AACACAAAGA
  181 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
  241 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
  301 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGAGAA GTCCTGGCGA CGCACAGTTT
  361 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA
  421 CCGAGTCGGT GCTTTTTTGT TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG
  481 TTTTTAGCGC GTGCGCCAAT TCTGCAGACA AATGGCTCTA GAGGTACCCG TTACATAACT
  541 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAGT
  601 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
  661 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
  721 TAAATGGCCC GCCTGGCATT GTGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
  781 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCNAN GTGAGCCCCA CGTTCTGCTT
  841 CACTCTNNNC ATCTCCCCCC CCCTCCCCAC CCCCNATTTT GTATTNATTT ATTTTTTAAT
901 TATTTTGTGC ANNGATGGGG GCGGGGGGGG GGGGGGGNGN NNNNCNCNNN NNNNNNN
//
Primer: Cas9-N_R3 (Pr0754)
Region: Cas9,SV40 NLS,3xFLAG,CBh pro
Sequence file: RDB18355_B0Gfb.seq check
>D07105C5_B0Gf_3_cas9_N_R3_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NGCCGANGTC NNGGCCGANG CTGTACTTCT TGTCGGCTGC
   61 TGGGACTCCG TGGATACCGA CCTTCCGCTT CTTCTTTGGG GCCATCTTAT CGTCATCGTC
  121 TTTGTAATCA ATATCATGAT CCTTGTAGTC TCCGTCGTGG TCCTTATAGT CCATGGTGGC
  181 ACCGGTCCAA CCTGAAAAAA AGTGATTTCA GGCAGGTGCT CCAGGTAATT AAACATTAAT
  241 ACCCCACCAA CCAACCATCC CTTAAACCCT TACCTCTTGC TCAGCTAATT ACAGCCCGGA
  301 GGAGAAGGGC CGTCCCGCCC GCTCACCTGT GGGAGTAACG CGGTCAGTCA GAGCCGGGGC
361 GGGCGGCGCG AGGCGGNNGC NGANCNNNNN NNNGNNNNNA NGNNNNNNNN NN
//
Primer: b-actin pro_F1 (Pr1046)
Region: 3xFLAG,SV40 NLS,Cas9
Sequence file: RDB18355_B0Gfc.seq check
>D07105C5_B0Gf_3_b-actin pro_F1_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNT GNNTGGNGGG GTANTAATGT TTAATTACCT GGAGCACCTG
   61 CCTGAANNNN NNTTTTTTCA GGTTGGACCG GTGCCACCAT GGACTATAAN GNCCACGACG
  121 GAGACTACAA GGATCATGAT ATTGATTACA AAGACGATGA CGATAAGATG GCCCCAAAGA
  181 AGAAGCGGAA GGTCGGTATC CACGGAGTCC CAGCAGCCGA CAAGAAGTAC AGCATCGGCC
  241 TGGACATCGG CACCAACTCT GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA
  301 GCAAGAAATT CAAGGTGCTG GGCAACACCG ACCGGCACAG CATCAAGAAG AACCTGATCG
  361 GAGCCCTGCT GTTCGACAGC GGCGAAACAG CCGAGGCCAC CCGGCTGAAG AGAACCGCCA
  421 GAAGAAGATA CACCAGACGG AAGAACCGGA TCTGCTATCT GCAAGAGATC TTCAGCAACG
  481 AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACAGACTGGA AGAGTCCTTC CTGGTGGAAG
  541 AGGATAAGAA GCACGAGCGG CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC
  601 ACGAGAAGTA CCCCACCATC TACCACCTGA GAAAGAAACT GGTGGACAGC ACCGACAAGG
  661 CCGACCTGCG GCTGATCTAT CTGGCCCTGG CCCACATGAT CAAGTTCCGG GGCCACTTCC
  721 TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG
  781 TGCAGANCTA CAACCAGCTG TTCGAGGAAA ACCCCATCAA CGCCAGCGGC GTGGACGCCN
  841 AGGCCATCCT GTCTGCCNGA NTGANCAAGA NCAGANNGCT GGAAAATCTG ATCGCCNAGC
  901 TGCCCNGNGA NAAGAAGANT GGCCTGNNCG GAAACCNNAT TGCCCTNANC CNGGGCCTGA
961 NCCCNNACTT CNNNANCNNC TTCNNNNNNN NNN
//
Primer: bGH_rev3 (Pr0885)
Region: nucleoplasmin NLS,Cas9
Sequence file: RDB18355_B0Gfd.seq check
>D07105C5_B0Gf_3_bGH_rev3_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNGAT GGCTGGCAAC
   61 TAGAAGGCAC AGTCGAGGCT GATNNNNNNN GCTCTAGGAA TTCTTACTTT TTCTTTTTTG
  121 CCTGGCCGGC CTTTTTCGTG GCCGCCGGCC TTTTGTCGCC TCCCAGCTGA GACAGGTCGA
  181 TCCGTGTCTC GTACAGGCCG GTGATGCTCT GGTGGATCAG GGTGGCGTCC AGCACCTCTT
  241 TGGTGCTGGT GTACCTCTTC CGGTCGATGG TGGTGTCAAA GTACTTGAAG GCGGCAGGGG
  301 CTCCCAGATT GGTCAGGGTA AACAGGTGGA TGATATTCTC GGCCTGCTCT CTGATGGGCT
  361 TATCCCGGTG CTTGTTGTAG GCGGACAGCA CTTTGTCCAG ATTAGCGTCG GCCAGGATCA
  421 CTCTCTTGGA GAACTCGCTG ATCTGCTCGA TGATCTCGTC CAGGTAGTGC TTGTGCTGTT
  481 CCACAAACAG CTGTTTCTGC TCATTATCCT CGGGGGAGCC CTTCAGCTTC TCATAGTGGC
  541 TGGCCAGGTA CAGGAAGTTC ACATATTTGG AGGGCAGGGC CAGTTCGTTT CCCTTCTGCA
  601 GTTCGCCGGC AGAGGCCAGC ATTCTCTTCC GGCCGTTTTC CAGCTCGAAC AGGGAGTACT
  661 TAGGCAGCTT GATGATCAGG TCCTTTTTCA CTTCTTTGTA GCCCTTGGCT TCCAGAAAGT
  721 CGATGGGANT CTTCTCNAAN CTGCTTCTTT CCATGATGGT GATCCCCAGC AGCTCTTTCA
  781 NNNTCTTCAG TTTCTTGNAC TTGNCCNTTT TCCACNTTNN GNCNCCACCN NNNNNNAANA
  841 GGCCNCGGTG GGGCTGTCGA NNCCGCCGTA NTTCTTANGG NNNNNNTCNN TTCNTNCTGG
  901 NGANNANCNN NNNNCTNTTC CTCTTNGGCA GGNNANANTC NTTNGCTGAA NCCNCCTGTN
961 TGNACNTNNN NCTTTTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]