Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMK322

Donor plasmid to generate TOP2A-mAID degron cell lines with mClover as a marker, hygromycin resistance.

Sequence (full)	RDB18361zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18361_B1K1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739) Region: hTOP2A_1, linker, mAID 5' Sequence file: RDB18361_B1K1a.seq
>18361_18361_B1K1_3_pUC-CAPbs_up_F2_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNNNNNNN TTATGCTTCC GGCTCGTATG TTGTGTGGAA    61 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCG   121 GCGCGCCATT GGGATGGAAC GAAAACTCCT TGAATATAGT GTCTTGATAC ATTTTTTTAC   181 ATTACAATTA TGTTGTCAGA TTTACAATGT GCAAGTTACC TGGGCTTTTC TCTTTTAGAA   241 ATCCAAGGGG GAGAGTGATG ACTTCCATAT GGACTTTGAC TCAGCTGTGG CTCCTCGGGC   301 AAAATCTGTA CGGGCAAAGA AACCCATTAA ATACCTGGAA GAGTCAGATG AAGATGATCT   361 GTTTGGATCC GGTGCAGGCG CCAAGGAGAA GAGTGCTTGT CCTAAAGATC CAGCCAAACC   421 TCCGGCCAAG GCACAAGTTG TGGGATGGCC ACCGGTGAGA TCATACCGGA AGAACGTGAT   481 GGTTTCCTGC CAAAAATCAA GCGGTGGCCC GGAGGCGGCG GCGTTCGTGA AGGTATCAAT   541 GGACGGAGCA CCGTACTTGA NGAAAATCGA TTTGAGGATG TATAAAGCTA GCATGGTGAG   601 CAAGGGCGAN GANCTGTTCN CCGGGGTGGT GNCCATCCNG NNCGAGCTGG ANGGCGACNT   661 AAACNGNCNC NAGTTCNNCG TNCGCNGCNA GGGNNANGGC NANGCCNCNA NNNNNNNGCT 721 NANCCTGAAN TTNNNCNNNN NNNNNN //
Primer: pUC-M13_-100 (Pr0310) Region: hTOP2A_2, loxP-2, mPGK ter Sequence file: RDB18361_B1K1b.seq
>18361_18361_B1K1_3_pUC-M13_-100_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTTTCCNN GTCNCGACGT TGTAAAACGA CGGCCAGTGA    61 ATTGACGCGT ATTGGGATGG AACGCCCATG AGATGGTCAC TATTTAGACA GTATTATAAA   121 AAGCTAAAGA ACACTTGGGC TTTACTTCAC TTTGATGTCT TGTACTAAAA ACACCTTCCC   181 CAAACTAAAT TCAGAGGGGA GGAAGTTAAG AGCTTCAGGT AACTTTAAAA CCAGTCTTGG   241 GCTTGGTAAG ATAATTACTT AAAATAATCG CCTCACATTT TAAAACAGAT CATCTTCATC   301 TGACTCTGGA TCCTAGTGAA CCTCTTCGAG GGACCTAATA ACTTCGTATA GCATACATTA   361 TACGAAGTTA TATTAAGGGT TCCGGATCTC GACCAGCTTC TGATGGAATT AGAACTTGGC   421 AAAACAATAC TGAGAATGAA GTGTATGTGG AACAGAGGCT GCTGATCTCG TTCTTCAGGC   481 TATGAAACTG ACACATTTGG AAACCNNNNN NCTTAGAACC ACAAAGTGGG AATCAAGAGA   541 AAAACAATGA TCCCACGAGA GATCTATAGA TCTATAGATC ATGAGTGGGA GGAANGAGCT   601 GGNCCTTAAT TNGGNTTNGN NTGNTTAANT NANNATATCN NANTATGAAA NNNTATCNTA   661 AAGNANNNNN NNAAGAGCCT TCNNTAAAGA NNNNNNNTTN NTCTNATCCN NNCCCNNNCC 721 CCNNNCCCNN NNNNNNNCNN NNNNCTTNNN NNNNNNN //
Primer: PGKpro_R (Pr0793) Region: PGK pro, loxP-1. SV40 pA, mClover(A206K) 3' Sequence file: RDB18361_B1K1c.seq
>18361_18361_B1K1_3_PGKpro_R_C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNGNGTGCGA NGCCNGANGC CNNTTGTGTA GCGCCNAGTG    61 CCCAGCGGGG CTGCTAAAGC GCATGCTCCA GACTGCCTTG GGAAAAGCGC CTCCCCTACC   121 CGGTAGAATT TCGAGGTCGA GATCCTAAGC TTGGCTGGAC GTAAACTCCT CTTCAGACCT   181 AATAACTTCG TATAGCATAC ATTATACGAA GTTATATTAA GGGTTATTGA ATATGATCGA   241 CGCGTTAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG   301 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA   361 ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA   421 GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC   481 TAGATTACTT GTACAGCTCG TCCATGCCAT GTGTAATCCC GGCGGCGGTC ACGAACTCCA   541 GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGCTTGGAC TGATGGCTCA   601 GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT   661 GGTCGGCGAG CTGCACGCTG CCGTCCTCAA CGTTGTGGCG GATCTTGAAG TTAGCCTTGA   721 TGCCGTTCTT CTGCTTGTCG GCCGTGATAT AGACGTTGTG GCTGTTGAAG TTGTACTCCA   781 GCTTGTGCCN CANGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT   841 TCACCNNGGT GTCGCNCTCN AACTTCANCT CNGCGCGGGT CTTGNNNNNA CCNNCGNNNN   901 NGAAAGAGAN GGTGCGNTNN TGGACGTANC CNTCNGGCNT GGNNGANTTN NNNNNGNNGN 961 GCTNCNNCAT GNGNNNNGGG NANCNGNNTG NNNNNNNNNN //
Primer: PGKpro_F (Pr0249) Region: HygR Sequence file: RDB18361_B1K1d.seq
>18361_18361_B1K1_3_PGKpro_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNTCTC CNGGCCTTTC GACCTGCATC CNTCTAGATC TCGAGCAGCT    61 GAAGCTTACC GCTAGCATGG ATAGATCCGG AAAGCCTGAA CTCACCGCGA CGTCTGTCGA   121 GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA   181 AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG   241 CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT   301 CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC   361 CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT   421 GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG   481 GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG   541 CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG NTGGACGACA CCGTCAGTGC   601 GTCCGTCGCG CANGCTCTCG ATGANCTGAN GCTTTGGGCC GANGANTGNC CCNAAGTCCG   661 GCACCTCNNG CACNCNGNNT TCNGCTNCNN CNNTGNCCNG ANNNNNNNNN GNNNGCNTNN 721 NNNNNGTCNN NNNNNNNN //
Primer: EGFP-C-rev (Pr0026) Region: mClover(A206K) 5', mAID 3' Sequence file: RDB18361_B1K1e.seq
>18361_18361_B1K1_3_EGFP-C-rev_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTGGNN TGNNGGCTCN NNGNNNNGGT    61 TGTCGGGCAG CANCNCGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA   121 GCTGCACGCT GCCGTCCTCA ACGTTGTGGC GGATCTTGAA GTTAGCCTTG ATGCCGTTCT   181 TCTGCTTGTC GGCCGTGATA TAGACGTTGT GGCTGTTGAA GTTGTACTCC AGCTTGTGCC   241 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG   301 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGGT ACCGTCGTCC TTGAAAGAGA   361 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA   421 TGTGGTCGGG GTAGCGGCTG AAGCAGGCCA CGCCGTAGCC GAAGGTGGTC ACGAGGGTGG   481 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTTGG   541 TGGCATCGCC CTCGCCCTCG CCGCGGACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA   601 GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCTAG   661 CTTTATACAT CCTCAAATCG ATTTTCCTCN AGTACGGTGC TCCGTNNNNN GATACCTTCA   721 CGAACGCCGC CGCCTCCGGG CCACCGCTTG ATTTTTGGCA NGAAANCATC ACGTTCTTCC   781 GGTATGATCT CNNCGGNGGC CATCNCNCNN CTTGTGCCTT GGCCGGANGT TTGGCTGGAT   841 CTTTANGANA AGCACTCNTT CTCCTNNGCG NCNGCACCGG NNCCAAACAG NATCATCTTC   901 ATCTGACTNT NNNANGTATT TNANNGGGGN TTCTTTNNCC CGNNNNNGAT TTTNNNNNNN   961 AGGANNNNNC NNGNNGNANN NNNNANNNCN ANATNGNAAA NNCNNTNNNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl