RDB18571 - pSFL17 pRS315-GPDp-Ifa38-GFP1-10

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pSFL17 pRS315-GPDp-Ifa38-GFP1-10 (#RDB18571)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: endoplasmic reticulum.

Alternative name	pSFL17, pRS315-GPDp-Ifa38-GFP1-10
Clone info.	The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment	Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pRS315 (plasmid)
Selectable markers	Ampicillin (E. coli), LEU2 (S. cerevisiae).
Gene/insert name	S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Tamura, Yasushi \|

Sequence (full)	RDB18571zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18571	pSFL17 pRS315-GPDp-Ifa38-GFP1-10	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSFL17 pRS315-GPDp-Ifa38-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18571).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18571_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18571_B0J5a.seq
>D07393A5_B0J5_3_HB-NOS_R_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNC GCCNGGGTTT TCCCAGTCAC GACGTTGTAA 61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC 121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA 181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA 241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG 301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC 361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG 421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT 481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA 541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG 601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA 661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT 721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCATGAC 781 TTTTATGCAA CAGCTTCAAG ANGCTGGGGA ANGANTTAGG TGTATCAATG GTCTTTTATG 841 GNTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTANAT TTTTTAGCTC 901 TTATTTTTGA TCTTTTTTTT ACTACCAGCA GNCNANNTTC GANNNNNNTN GNGCTNAAAC 961 TGGNAAATNC TNNNCNATCA NNNGNNGCNN NNNNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3') Sequence file: RDB18571_B0J5b.seq
>D07393A5_B0J5_3_SV40pA3'ElementReverse_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNGNNCCN ANGNNNNNGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC 61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA 121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG 181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG 241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA 301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT 361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG 421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT 481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT 541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA 601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG 661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC 721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC 781 TTACTTTTCG TTGGGATCTT TCGAAAGGAC AGTTTGTGTC GACAGGTAAT GGTTGTCTGG 841 TAAAAGGACA GGGCCATCGC CNATTGGANT ATTTTGTTGA TNATGGNCTG CTANTTGAAN 901 GGAACCATCT NNNNNGNNNN GNCNAANNNN GAANTTAGCN TTGANTNCNT TCTTTTNNTT 961 GNCNGNCNNN NNGNNNNNNN NNN //
Primer: paGFP-M_R1 (Pr0893) Region: insert mid (GFP1-10 5', Ifa38 3') Sequence file: RDB18571_B0J5c.seq
>D07393A5_B0J5_3_paGFP-M_R1_D03_12_ABI24.ab1 1 NNNNNNNNNN NNANNCNTGC CTTTTNNTGT GATCCGGANN NACGGGAAAA GCATTGAACA 61 CCATAGGTCA GAGTAGTGAC AAGTGTTGGC CATGGAACAG GTAGTTTTCC AGTAGTGCAA 121 ATAAATTTAA GTGTGAGTTT TCCGATTGTA GCATCACCTT CACCCTCTCC TCTGACAGAA 181 AATTTGTGCC CATTAACATC ACCATCTAAT TCAACAAGAA TTGGGACAAC TCCAGTGAAA 241 AGTTCTTCTC CTTTGCTCAT ACTAGTGCCA CCCATTGATC CGCCACCAGA CCCTCCGGAT 301 CCTTCCTTTT TAACCTGTCT TGCGGCTTTT TTTAAGGCTC TAATTCTGAT AGATTTATGG 361 AAGGAATAAT TAATGGAGTT AACAATCTTA GAGTAAACGC CAAAGGTCTC TGTAATTACA 421 AATTGATACA CTGCGTGCGC CCAGTAAGGG GTCATAGTAG CGTATCTTTC TTGAGAGCCG 481 CAGCGTCTGC CAACGCTTCT TAAAGTGGAT TTTACAAACT GTTGTGGATT TGGGATCATT 541 AAAGATGATC TTCTGATTTT AGACATCGAG CTAGTGACCA AATATGAAAT GATTAATTCA 601 ACATCGATAG CATCTTTAGA TAATTCCCCA GCCAAAGAGT TAGACCAACC TTGTAAGAAT 661 GATTTCGAAC CACTGTATGT AGCCAAAAGT GGGGTGGGAA TCAGACCACC AAATGAGCCC 721 ATGGTTAAGA TCAAACCACG AGTACCCGAC TTCTTGTTTT CAGCTTTCAC AGTTTCCACA 781 ATCTTTGGTG CAATGATTTG TGTAATTAAT AATGTANCAG TGTTATTGAT AGTGATAATA 841 TTTCTAAGCT CCTTCTCTTC TGTTTCCAAA AATGGAACAG GANTNNAGTG TGATTGANNN 901 ACATTATTGA NCNAAACGGT GATNNGNAAC TGCGCACACA ATTCCTTNAN NNNNTCATAN 961 TTGNNNTCTT TATCCNNCGC NANGNCNNNG GCTANNNNNN TTNNNNNNNN NN //
Primer: LEU2pro_F (Pr0169) Region: LEU2 pro, LEU2 Sequence file: RDB18571_B0J5d.seq
>D07393A5_B0J5_3_LEU2pro_F_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNATT NANGGNNCTA TTGTTTTTTC NNATAGGTGG TTAGCAATCG 61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA 121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT 181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC 241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT 301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT 361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA 421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT 481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC 541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC 601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT 661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT 721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGANGAAA CCATCAAGAA CGAATTCCCT 781 ACATTGAANG NTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA 841 ACCCACCTAA NTGGTATTAT AATCACCAGC AACATGNTTG GTGANATCAT CTCCNATGAA 901 GCCTCCGTNA TCCCANGNTC CTNNGGNTNG NTGCCNTCNG CNNCCNNGGN CNCTTTGCCN 961 GANAAGAANN CCGCATTTNG NTTNGNNCNA NNCNNTGNNN NNGNNNCNGN TNNNNNNTTN 1021 NCNNAANNNN NNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18571_B0J5e.seq
>D07393A5_B0J5_3_TDH3pro_F_E03_15_ABI24.ab1 1 NNNNNNNNNN NGNNNTGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA 61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG 121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG 181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT 241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG 301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG 361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT 421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA 481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT 541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT 601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT 661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT 721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC 781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG 841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GNCACTAGCT 901 CGATGNCTAA AATCNNAANA TCATCTTTAA TGATCCNNAA NNCNNNANNN NTTGNAAAAT 961 CCANTTTNAN NNNNNNNNGN NANNNNNNNN NN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP1-10) Sequence file: RDB18571_B0J5f.seq
>D07393A5_B0J5_3_CYC1ter_R_F03_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT 61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT 121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG 181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT 241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA 301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT 361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA 421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT 481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG 541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT 601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT 661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA 721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC 781 CAGANCCTCC GGANCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAN GNTCTAATTC 841 TGATAGATTT ATGGAAGGAA TAATTAATGN ANTTAACAAT CNNANANTAA ANGCCAANNN 901 TCTCTGTAAT TACNAATNNA TACNCTGCGN GCGCCCANNA ANGGGNCATA NNANCGNATC 961 TTTCTTGANA NCNNCANCNN CNGNNNNCGC TTNNNNAAGN NNNNTTNNNN NNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18571_B0J5g.seq
>D07393A5_B0J5_3_Amp_R_G03_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNTGAN NANTTATCNG GGTTATTGTC TCATGAGCGG ATACATATTT 61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA 181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC 241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC 301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA 361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT 421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT 481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC 541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA 601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCCTAAGAAA CCATTATTAT 661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA 781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGNGTCG 841 GGGCTGGCTT AACTATGCGG CATCNNANCA GATNGTACTG ANAGTGNACC ATATCGACNA 901 CNTCGTTAAN GNCGTTTCTG ANANANTAAA ANTNTTGANG GAACTTTCNC NNTTANNGGN 961 AANGNNTCNN GNNNNNNNTG ANTNNAACTN NNNCNANNNN NCNNNNCNNN NNNNNNTTTT 1021 NNNTNNNNNN NNTTTTTTTT TTTTTTTNNN ANNNNNNNNN N //

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References

Original, user report and related articles

original	Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]