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pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 (#RDB18572)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: mCherry-GFP11; location: endoplasmic reticulum.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kakimoto, Y., Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pSFL61, pRS316-GPDp-Ifa38-mCherry-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Growth conditions LB+Ampicillin, 37oC
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA Discosoma sp RFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。

Catalog # Resource name Shipping form Fee
RDB18572 pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18572).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [PubMed] [Article] [RRC of NBRP]