RDB18574 - pSFL68 pRS314-GPDp-Erg6-V5-GFP11

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pSFL68 pRS314-GPDp-Erg6-V5-GFP11 (#RDB18574)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: lipid droplets.

Alternative name	pSFL68, pRS314-GPDp-Erg6-V5-GFP11
Clone info.	The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment	Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pRS314 (plasmid)
Selectable markers	Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name	S. cerevisiae ERG6 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Tamura, Yasushi \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18574	pSFL68 pRS314-GPDp-Erg6-V5-GFP11	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSFL68 pRS314-GPDp-Erg6-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18574).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18574_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB18574_B0J5a.seq
>D07393A8_B0J5_3_HB-NOS_R_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNAACGCC NGGGTTTTCC CAGTCACGAC 61 GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCA 121 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT 181 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC 241 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT 301 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA 361 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT 421 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCAATG 481 GAGTGATGCA ACCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC 541 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGATTTGGA AAAAGCTGAA 601 AAAAAAGGTT GAAACCAGTT CCCTGAAATT ATTCCCCTAC TTGACTAATA AGTATATAAA 661 GACGGTAGGT ATTGATTGTA ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT 721 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC NGANGCGGCC 781 GCNTGANTGA AACAGAATTG AGAAAAAGAC AGGNCCNATT CACTANGGAG TTACATGGTG 841 ATGATATTGG TAAAAAGANN GGNTTGANNG CATTGATGTC GAANNAACAA CTCTGCNCNA 901 ANNNNANCCG TNNNNAANTA CTTGANAAAT TGGGNNGGNA GAANCGATNA ANANGCCNNA 961 NAANNNCNTC NTGNNNNANT ATANNGNNNN CNCNCNNTNC CTACNNNNAN NNNNTTNNNN 1021 ANNNNNNNNN N //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3') Sequence file: RDB18574_B0J5b.seq
>D07393A8_B0J5_3_SV40A3'ElementReverse_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNAANNCNNG CAACGCGGCC TTTTTACGGT 61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG 121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG 181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC 241 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG 301 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC 361 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG 421 GAAACAGCTA TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA 481 AGCTGGTACC GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT 541 TTCAGTATAA TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT 601 ATAAATAACG TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA 661 GGTTGTCTAA CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG 721 CGTGACATAA CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT 781 CTTANGTAAT GCCTGCAGCA TTGACATATT CGTGTAGAAN CATATGATCT CTGCTTGTGG 841 AACCNCCACC ACTTCCNCCA GTTGAGTCTA AGCCCNAAAN ANGGTTNNGT ATGGGTTTCC 901 CANAANCCTC CGGCNNANGN NCCTNGANTT GCTNNTTGGG AANTTTGGGA NGGGGNTNCG 961 GNGTTTTCTG GNNTNNNNAN CNNNNAAAAG CATCATNNGN NNNGNNNNAN NTTNGNNNNN 1021 NNNNN //
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18574_B0J5c.seq
>D07393A8_B0J5_3_TRP1pro_F_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNAGGCAGCT TGGANTANGT CTGTTATTAA TTTCNCAGGT 61 AGTTCTGGTC CNTTGGTGAA AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCT 121 CTAGATTCCG ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT 181 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC 241 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG 301 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCAAGAATAC 361 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA 421 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA 481 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG AAGGCAAGAG 541 AGCCCCGAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT 601 GCGCTTAGAT TAAATGGCGT TATTGGTGTT GATGTAAGCG GAGGTGTGGA GACAAATGGT 661 GTAAAAGACT CTAACAAAAT AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT 721 GAGTAGTATT TATTTAAGTA TTGTTTGTGC ACTTGCCGAT CTATGCGGTG TGAAATACCG 781 CACAGATGCG TAAGGAGAAA ATACCGCATC ANGAAATTGT AAGCGTTAAT ATTTTGTTAA 841 AATTCGCGTT AAATTTTTGT NNAATCAGCT CATTTTTTAA CCAATANGCC GAAATCGGCA 901 AAATCCCTTA TAAATCNAAA GAATANANCG ANATAGGGTT GANTGNTGNT CCNGTTTGGN 961 ANNAGAGTNN NCTATTAAAG AACGNGNNNN NNNNCNNNNA NNNNNAAAAA NCGNCTATCN 1021 NNNNNNNGNN NNNNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, V5-GFP11, Erg6 3') Sequence file: RDB18574_B0J5d.seq
>D07393A8_B0J5_3_CYC1ter_R_F03_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GCGGNNGNGG GGGGAGGGCG TGAATGTAAG CGTGACATAA 61 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTAGGTAAT 121 GCCTGCAGCA TTGACATATT CGTGTAGAAC CATATGATCT CTGCTTGTGG AACCACCACC 181 ACTTCCACCA GTTGAGTCTA AGCCCAAAAG AGGGTTTGGT ATGGGTTTCC CAGAACCTCC 241 GGCAGAGGAT CCTTGAGTTG CTTCTTGGGA AGTTTGGGAG GGGGTTTCGG CGTTTTCTGG 301 CTTCCTAGCG ACGAAAAGCA TCATTGGAGT GAATAACTTG GACTTACCAC CGGCAACTAA 361 ACCAACCGCA GCATTTTCTA GAGCAGCAGT AACTTCCTTG GAACCTTCTG GGGCTAGACC 421 TAATTTTTCC ATTACAGTAA CCATTGCTGT AGTAAATTGT CTACCCAAGT AAGAAGTTCT 481 GAAAAATGTG GCCAAATTAG CTAAGTTTTG AACGTACTTC CACTCACCAG TTAATGGGTA 541 ATACCAAGGG ATTTCATCAT CATTGTCCGC CAGGTCTTCG CTAACGAGGA CTTCGAAACC 601 ACAGTTCTTC AATGCTTTCC TAGCCACGTC GACATGGAAC ATCTTTGGGA TACCATCACC 661 TAGTTCAATT TCATAAGCGA TCTTTCTATG TTCAGGATTG TTTTCGTCAT ATTTATCAGT 721 CATTACCCAT TCGTAAACAG CAAAGGTANC ACCCGGTTTC AAAACCTTGT AGATTTCGCT 781 GTATACACCT TCTAATTTTG GAGCGTGACA TGTGGCCTCA ATTGCATAAA CTTTGTCNAA 841 AGTGTTTTCT TCGAAATCCA TTTTCNTGAA ATCACCCTTT ACAAAGTCCA TTTGGTCANT 901 CAAATTGTAT TTTTTTANCG NAATATTTTG NCNNNNCAAT TTGGNAATCG TTATTGNTTA 961 NACNNATGAN GTTNCANNCN GNNAATNNNG NNNTCTNNNT NGNTGGGNCC NNNNCACNNN 1021 NNNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Erg6 5') Sequence file: RDB18574_B0J5e.seq
>D07393A8_B0J5_3_TDH3pro_F_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTGNNNTTCN GNAAATCTAT TTCTTAAACT TCTTAAATTC 61 TACTTTTATA GTTAGTCTNT NNTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG 121 CGGCCGCATG AGTGAAACAG AATTGAGAAA AAGACAGGCC CAATTCACTA GGGAGTTACA 181 TGGTGATGAT ATTGGTAAAA AGACAGGTTT GAGTGCATTG ATGTCGAAGA ACAACTCTGC 241 CCAAAAGGAA GCCGTTCAGA AGTACTTGAG AAATTGGGAT GGTAGAACCG ATAAAGATGC 301 CGAAGAACGT CGTCTTGAGG ATTATAATGA AGCCACACAT TCCTACTATA ACGTCGTTAC 361 AGATTTCTAT GAATATGGTT GGGGTTCCTC TTTCCATTTC AGCAGATTTT ATAAAGGTGA 421 GAGTTTCGCT GCCTCGATAG CAAGACATGA ACATTATTTA GCTTACAAGG CTGGTATTCA 481 AAGAGGCGAT TTAGTTCTCG ACGTTGGTTG TGGTGTTGGG GGCCCAGCAA GAGAGATTGC 541 AAGATTTACC GGTTGTAACG TCATCGGTCT AAACAATAAC GATTACCAAA TTGCCAAGGC 601 AAAATATTAC GCTAAAAAAT ACAATTTGAG TGACCAAATG GACTTTGTAA AGGGTGATTT 661 CATGAAAATG GATTTCGAAG AAAACACTTT CGACAAAGTT TATGCAATTG AGGCCACATG 721 TCACGCTCCA AAATTAGAAG GTGTATACAG CGAAATCTAC AAGGTTTTGA AACCGGGNGG 781 TACCTTTGCT GTTTACGANT GGGTAATGAC TGATAAATAT GACGAAAACA ATCCTGAACA 841 TAGAAAGATC GCTTATGAAA TTGANCTANG TGANGGTATC CCNAAGANGT TNCANGTCNA 901 CGNGGCTAGG AAAGCATTGA AGAANTGTGN TTTCNAAGTC CTCGTTAGCG ANNNNNNNNN 961 N //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18574_B0J5f.seq
>D07393A8_B0J5_3_Amp_R_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNATTTATCN GGGTTATTGT CTCATGAGCG GATACATATT 61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA 181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT 241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT 301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG 361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC 421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT 481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT 541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT 601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT 661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG 841 GGGCTGGCTT AACTATGCGG CATCNNANCA GANTGNACTG AGANNGNNCC NTANATCACG 901 ANATTACTAT ATATATAATA TAGGAAGCAT TTAATANAAN NGCATCGTAA TATATGTGTA 961 CTTTGCAGTT ATGANNNNNN ANGNNANNAN NGNNNNANAN NCNTTANNGN AAAATANNNN 1021 GNNNCNNTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]