Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL73 pRS314-GPDp-3xFLAG-GFP11

Expression vector of 3xFLAG-GFP11.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18575_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, 3xFLAG-GFP11 5') Sequence file: RDB18575_B0J5a.seq
>D07393A9_B0J5_1_HB-NOS_R_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN ANNNNGGGTN NNGCCNGGGT TTTCCCNGTC NCGACGTTGT    61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA   121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT   181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA   241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC   301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA   361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG   421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG   481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT   541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA   601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG   661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA   721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCTCT   781 AGAACTAGTG GATCCTCTGC CGGTGGTGAN TACAAAGACC ATGATGGCGA TTATAAGGAT   841 CATGACATTG ACTATAANGA TGATGACGAT AAAGGNGGAA GTGGTGGANG NTCAACTTNC   901 AGANATCACA TGGNTTTGNA TGNNNACGTC ANNGNTGCNN NGNNNNANAT AAGAATTCNA   961 NNTCNNGCTT ATCGANANNN NCNANCNNCN NNNCNNNNNA NNNNNNTNNN GNNNNNNNTN 1021 NNNNTNNNNN NCCNNNCNCN CNNNNNNNNN N //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3') Sequence file: RDB18575_B0J5b.seq
>D07393A9_B0J5_1_SV40A3'elementReverse_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCCNGCNAC GCGGCCTTTT TACGGTTCCT    61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA   121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG   181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC   241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG   301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT   361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA   421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT   481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA   541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA   601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT   661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG   721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA   781 TGTTATCCCT GCAGCATTGA CGTATTCATG CNAAACCNTG TGATCTCTGG AAGTTGANCC   841 NCCACCACTT CCNCCTTTAT CGNCATCATC CTTATAGTCN ANGTCNTGAT CCNTNATAAT   901 CGCCNTCNTG GNCTTTGNNA TCNNCANCGG NANANGNNCC NCNANTTCTA GANCGGCCGC   961 NTCCNNCGAA ACTAANTTNT GGNGTTTTAA AACTNAAAAA AAANNANTNN NNNNAAANNN 1021 NNNNNNN //
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18575_B0J5c.seq
>D07393A9_B0J5_1_TRP1pro_F_G04_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNAGNNNN GCTTGGANTN NGTCTGTTAT TAATTTCACA    61 GGTAGTTCTG GTCCATTGGT GAAAGTTTGC GGCTTGCAGA GCACAGAGGC CGCAGAATGT   121 GCTCTAGATT CCGATGCTGA CTTGCTGGGT ATTATATGTG TGCCCAATAG AAAGAGAACA   181 ATTGACCCGG TTATTGCAAG GAAAATTTCA AGTCTTGTAA AAGCATATAA AAATAGTTCA   241 GGCACTCCGA AATACTTGGT TGGCGTGTTT CGTAATCAAC CTAAGGAGGA TGTTTTGGCT   301 CTGGTCAATG ATTACGGCAT TGATATCGTC CAACTGCATG GAGATGAGTC GTGGCAAGAA   361 TACCAAGAGT TCCTCGGTTT GCCAGTTATT AAAAGACTCG TATTTCCAAA AGACTGCAAC   421 ATACTACTCA GTGCAGCTTC ACAGAAACCT CATTCGTTTA TTCCCTTGTT TGATTCAGAA   481 GCAGGTGGGA CAGGTGAACT TTTGGATTGG AACTCGATTT CTGACTGGGT TGGAAGGCAA   541 GAGAGCCCCG AAAGCTTACA TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT   601 GATGCGCTTA GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT   661 GGTGTAAAAG ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT   721 ACTGAGTAGT ATTTATTTAA GTATTGTTTG TGCACTTGCC GATCTATGCG GTGTGAAATA   781 CCGCACAGAT GCGTAANGAG AAAATACCGC ATCANGAAAT TGTAAGCGTT AATATTTTGT   841 TAAAATTCGC GTTAAATTTT TGTTAANTCA GCTCATTTTT TTAACCNANA GGNCNAANTC   901 GGCAAANTCC NTTATAAATC AAAAGANTAG ANCGANATAG GGTTGANTGN TGNTNCNGTT   961 TGNNNNNNGN NTNNNCTATT AAANAANGNN NNNTNNNNCN NCNANNNNNN AAAAANCNNN 1021 NNNTNNNNNN ANNNNNNNNN N //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, 3xFLAG-GFP11, GPD pro) Sequence file: RDB18575_B0J5d.seq
>D07393A9_B0J5_1_CYC1Ter_R_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CGGNNGTGGG GGGNNGGCGT GNNTGTAAGC GTGACATAAC    61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTTATC   121 CCTGCAGCAT TGACGTATTC ATGCAAAACC ATGTGATCTC TGGAAGTTGA ACCTCCACCA   181 CTTCCGCCTT TATCGTCATC ATCCTTATAG TCAATGTCAT GATCCTTATA ATCGCCATCA   241 TGGTCTTTGT AATCACCACC GGCAGAGGAT CCACTAGTTC TAGAGCGGCC GCATCCGTCG   301 AAACTAAGTT CTGGTGTTTT AAAACTAAAA AAAAGACTAA CTATAAAAGT AGAATTTAAG   361 AAGTTTAAGA AATAGATTTA CAGAATTACA ATCAATACCT ACCGTCTTTA TATACTTATT   421 AGTCAAGTAG GGGAATAATT TCAGGGAACT GGTTTCAACC TTTTTTTTCA GCTTTTTCCA   481 AATCAGAGAG AGCAGAAGGT AATAGAAGGT GTAAGAAAAT GAGATAGATA CATGCGTGGG   541 TCAATTGCCT TGTGTCATCA TTTACTCCAG GCAGGTTGCA TCACTCCATT GAGGTTGTGC   601 CCGTTTTTTG CCTGTTTGTG CCCCTGTTCT CTGTAGTTGC GCTAAGAGAA TGGACCTATG   661 AACTGATGGT TGGTGAAGAA AACAATATTT TGGTGCTGGG ATTCTTTTTT TTTCTGGATG   721 CCAGCTTAAA AAGCGGGCTC CATTATATTT AGTGGATGCC AGGAATAAAC TGTTCACCCA   781 GACACCTACG ATGTTATATA TTCTGTGTAA CCCGCNCCCT ATTTTGGGCA TGTACGGGTT   841 ACAGCANAAT TNAAANGNTA ATTTTTTTGA CTAAATAAAG NTAGGAAAAT CACNACTANT   901 AATTATTTAN NNANTCTTTG AAANGGNNNN NANNGATANN GANAAACTGA NCTNNNNTTC 961 NNCCNATANN GANTCNNANN ANNNTNNNNN NGNNNNNCNN TTNNNNANNN NNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18575_B0J5e.seq
>D07393A9_B0J5_1_Amp_R_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNANN NNANNCNTTN ATNNGGGTTA TTGTCTCNTG AGCGGATACA    61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG   121 TGCCACCTGG GTCCTTTTCA TCACGTGCTA TAAAAATAAT TATAATTTAA ATTTTTTAAT   181 ATAAATATAT AAATTAAAAA TAGAAAGTAA AAAAAGAAAT TAAAGAAAAA ATAGTTTTTG   241 TTTTCCGAAG ATGTAAAAGA CTCTAGGGGG ATCGCCAACA AATACTACCT TTTATCTTGC   301 TCTTCCTGCT CTCAGGTATT AATGCCGAAT TGTTTCATCT TGTCTGTGTA GAAGACCACA   361 CACGAAAATC CTGTGATTTT ACATTTTACT TATCGTTAAT CGAATGTATA TCTATTTAAT   421 CTGCTTTTCT TGTCTAATAA ATATATATGT AAAGTACGCT TTTTGTTGAA ATTTTTTAAA   481 CCTTTGTTTA TTTTTTTTTC TTCATTCCGT AACTCTTCTA CCTTCTTTAT TTACTTTCTA   541 AAATCCAAAT ACAAAACATA AAAATAAATA AACACAGAGT AAATTCCCAA ATTATTCCAT   601 CATTAAAAGA TACGAGGCGC GTGTAAGTTA CAGGCAAGCG ATCCGTCTAA GAAACCATTA   661 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT   721 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC   781 TGTAAGCGGA NGCCGGGAGC AGANAAGCCC GTCNGGGCGC GTCAGCGGGT GTTGGCGGGN   841 GTCGGGGCTG GCTTAACTAT GCGGCNTCNN ANCANANNGN ACTGANAGTG NNNCATAGAT   901 CACGANATTA CTATATATAT NATATAGGAA NNCATTTNAT AGAANNNCNT CGNAATATAT   961 GTGTANTTTG CNGTTATGNN GCCNNANGGN NNTANTGNNN NANATTCTTT ANTGAAAAAN 1021 ANNNNGNNNN CNTNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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