Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL74 pRS314-GPDp-V5-GFP11

Expression vector of V5-GFP11.

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18576_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB18576_B0J5a.seq
>D07393B1_B0J5_2_HB-NOS_R_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNN NNNNGGGTNN NGCCNGGGTT TTCCCNGTCA CGACGTTGTA    61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT   121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA   181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT   241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT   301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT   361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC   421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA   481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC   541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA   601 GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT   661 AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG   721 TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCTCTA   781 GAACTAGTGG ATCCTCTGCC GGANGTTCTG GGAAACCCAT ACCAAANCCT CTTTTGGGCT   841 TAGANTCNAC TGGTGGAANN GGNGGNGGNT NCNCAAGCAG ANATCATATG GNTCTACNCN   901 AATATGNCNN NGCTGCANGN ATTANCTAAN AATTCNANAT CNAGCNTATC GATANNNNCN   961 ANNNNNANNC ANGNNNNTAN NTATGNNNNG CNNNNNTTCN NGCCCNNCCN CNNNNNNNNN 1021 N //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3') Sequence file: RDB18576_B0J5b.seq
>D07393B1_B0J5_2_SV40A3'elementReverse_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA NGCCNGCAAC GCGGCCTTTT TACGGTTCCT    61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA   121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG   181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC   241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG   301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT   361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA   421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT   481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA   541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA   601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT   661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG   721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA   781 GGTAATGCCT GCAGCATTGA CATATTCGTG TAGAANCATA TGATCTCTGC TTGTGGAACC   841 ACCACCACTT CCACCAGTTG AGTCTAAGCC NNAAAGANGG TTNGGNATGG GTTNCCCAGA   901 ACCNCCNGCA GAGGANNNAC TANTNCNANN NCGGCCGCAT CCGNCGAANN TAANNTCTGG   961 NGNTTTAAAA CTNANAAAAA ANNNTNNNTA NNAAANNANA ANTTTNANNN NNNTNNNNNN 1021 NNNN //
Primer: TRP1pro_F (Pr0171) Region: TRP1, f1 ori Sequence file: RDB18576_B0J5c.seq
>D07393B1_B0J5_2_TRP1pro_F_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCAGC TTGGAGTANG TCTGTTATTA ATTTCNCAGG    61 TAGTTCTGGT CCNTTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC   121 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT   181 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG   241 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT   301 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA   361 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT   421 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC   481 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA   541 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA   601 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG   661 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC   721 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCGA TCTATGCGGT GTGAAATACC   781 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGAAATTG TAAGCGTTAA TATTTTGTTN   841 AAATTCGCGT TNAATTTTTG TTAAATCANC TCATTTTTTA ANNNATAGGN CGAAATCNNN   901 AAAATCCCTT ATAAATCAAA AGAATAGANC GAGATAGGGT TGAGNGNTGN TCCNGTTTNN   961 NNNNNGANTC CNCTNNTAAN NAACGNGNAN NNNNACNTCN ANNGNCGAAA AANNGTCNAT 1021 CNNNNNANNN NCNNNNACNN NNNNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, V5-GFP11, GPD pro) Sequence file: RDB18576_B0J5d.seq
>D07393B1_B0J5_2_CYC1Ter_R_D05_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN AGNGGNNGTG GGGGGANGGC GTGAATGTAA GCGTGACATA    61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA   121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC   181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC   241 CGGCAGAGGA TCCACTAGTT CTAGAGCGGC CGCATCCGTC GAAACTAAGT TCTGGTGTTT   301 TAAAACTAAA AAAAAGACTA ACTATAAAAG TAGAATTTAA GAAGTTTAAG AAATAGATTT   361 ACAGAATTAC AATCAATACC TACCGTCTTT ATATACTTAT TAGTCAAGTA GGGGAATAAT   421 TTCAGGGAAC TGGTTTCAAC CTTTTTTTTC AGCTTTTTCC AAATCAGAGA GAGCAGAAGG   481 TAATAGAAGG TGTAAGAAAA TGAGATAGAT ACATGCGTGG GTCAATTGCC TTGTGTCATC   541 ATTTACTCCA GGCAGGTTGC ATCACTCCAT TGAGGTTGTG CCCGTTTTTT GCCTGTTTGT   601 GCCCCTGTTC TCTGTAGTTG CGCTAAGAGA ATGGACCTAT GAACTGATGG TTGGTGAAGA   661 AAACAATATT TTGGTGCTGG GATTCTTTTT TTTTCTGGAT GCCAGCTTAA AAAGCGGGCT   721 CCATTATATT TAGTGGATGC CAGGAATAAA CTGTTCACCC AGACACCTAC GATGTTATAT   781 ATTCTGTGTA ACCCGCCCCC TATTTTGGGC ATGTACGGGT TACAGCAGAA TTAAAAGGCT   841 AATTTTTTGA CTAAATAAAG TTAGGAAAAT CACTACTATT NATTATTTAC GTATTCTTTG   901 AAATGNCGAG TANTGATAAT GATAAACTGA NCTCCNATTC GCCCTATAGN GAGNCGNATT   961 ACAATTCANN GGNCNNNCNT TTTNCNNCGT NNNNNNNGGG AAAACCNNNN NNNTNCCCNA 1021 NCNTNNNNNN NNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18576_B0J5e.seq
>D07393B1_B0J5_2_Amp_R_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNTNNNT GNNNCANTTA TNNGGGTTAT TGTCTCNTGG ANNCGGATAC    61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA   121 GTGCCACCTG GGTCCTTTTC ATCACGTGCT ATAAAAATAA TTATAATTTA AATTTTTTAA   181 TATAAATATA TAAATTAAAA ATAGAAAGTA AAAAAAGAAA TTAAAGAAAA AATAGTTTTT   241 GTTTTCCGAA GATGTAAAAG ACTCTAGGGG GATCGCCAAC AAATACTACC TTTTATCTTG   301 CTCTTCCTGC TCTCAGGTAT TAATGCCGAA TTGTTTCATC TTGTCTGTGT AGAAGACCAC   361 ACACGAAAAT CCTGTGATTT TACATTTTAC TTATCGTTAA TCGAATGTAT ATCTATTTAA   421 TCTGCTTTTC TTGTCTAATA AATATATATG TAAAGTACGC TTTTTGTTGA AATTTTTTAA   481 ACCTTTGTTT ATTTTTTTTT CTTCATTCCG TAACTCTTCT ACCTTCTTTA TTTACTTTCT   541 AAAATCCAAA TACAAAACAT AAAAATAAAT AAACACAGAG TAAATTCCCA AATTATTCCA   601 TCATTAAAAG ATACGAGGCG CGTGTAAGTT ACAGGCAAGC GATCCGTCTA AGAAACCATT   661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT   721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT   781 CTGTAAGCGG ANGCCGGGAG CAGANAAGCC CGTCAGGGCG CGTCAGCGGG NGTTGGNGGG   841 TGTCGGGGCT GGCTTAACTA TGCGGNATCN GANCANANTG NACTGANAGT GCNCCATANA   901 TCACGACNTT ACTATATATA TAATATAGGA AGCATTTAAT ANAACAGCAT CGNAATANAT   961 GTGNACNTTG CANTNNNANG CNNGANGGCA NTNNNNNNNA NNTNCTTTAN NGNAAAATAN 1021 NTTGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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