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PKINES-venusx2-SV40 NLS/pcDNA

Expression vector of an NLS from SV40 and NES from human PKI (PKI NES-2x Venus-SV40 NLS).

Catalog number RDB18605
Resource name PKINES-venusx2-SV40 NLS/pcDNA
Clone info. Expression vector of an importin-alpha/beta-dependent classical nuclear localization signal (NLS) from SV40 and nuclear export signal (NES) from human cAMP-dependent protein kinase inhibitor alpha (PKI) tagged with 2x Venus (PKI NES-2x Venus-SV40 NLS).
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Human PKIA cDNA
Depositor|Developer Imaoka,, Susumu |
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External Database
Human PKIA
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)。
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          RDB18605 PKINES-venusx2-SV40 NLS/pcDNA Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The PKINES-venusx2-SV40 NLS/pcDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18605).

          Reference section:

          Oguro, A., Imaoka, S., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Oguro, A., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

          2023.11.23

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