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PKINES-venusx2-PTHrP NLS/pcDNA

Expression vector of an NLS from PTHrP and NES from human PKI (PKI NES-2x Venus-PTHrP NLS).

Catalog number RDB18606
Resource name PKINES-venusx2-PTHrP NLS/pcDNA
Clone info. Expression vector of an importin-beta-dependent nuclear localization signal (NLS) from PTHrP and nuclear export signal (NES) from human cAMP-dependent protein kinase inhibitor alpha (PKI) tagged with 2xVenus (PKI NES-2x Venus-PTHrP NLS).
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Human PKIA cDNA Human PTHLH cDNA
Depositor|Developer Imaoka,, Susumu |
Other clones in our bank
External Database
Human PKIA Human PTHLH
        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)。
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        RDB18606 PKINES-venusx2-PTHrP NLS/pcDNA Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        How to cite this biological resource

        Materials & Methods section:

        The PKINES-venusx2-PTHrP NLS/pcDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18606).

        Reference section:

        Oguro, A., Imaoka, S., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)

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        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Oguro, A., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

        2023.11.23

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