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pCW DNAseIIα-sfGFP-T2A-mCherry (#RDB18608)

Lentiviral expression vector of lysosomal-METRIQ (MEasurement of protein Transporting integrity by RatIo Quantification) probe.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ishii, S., Sci. Rep. 9 (1): 11635 (2019). PMID 31406169. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name Lysosomal-METRIQ
Clone info. Lentiviral expression vector of DNaseII alpha-sfGFP-T2A-mCherry for lysosomal-METRIQ (MEasurement of protein Transporting integrity by RatIo Quantification) probe, see Fig. 1 of Ishii, S. et al., Sci. Rep., 9 (1): 11635, 2019.
Comment For expression in mammalian cells, 1 to 3 microgram/mL doxycycline is required. Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pCW57.1 (plasmid)
Size of vector backbone 9.4 kb
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human DNASE2 cDNA
Depositor|Developer Itakura, Eisuke |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, S et al. Sci Rep. 9 (1), 11635, 2019)
Remarks Remember that you will be working with samples containing infectious virus.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, S et al. Sci Rep. 9 (1), 11635, 2019)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB18608 pCW DNAseIIα-sfGFP-T2A-mCherry DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pCW DNAseIIα-sfGFP-T2A-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18608).

Reference section:

Ishii, S., Matsuura, A., Itakura, E., Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci. Rep. 9 (1): 11635 (2019). PMID 31406169. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ishii, S., Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe. Sci. Rep. 9 (1): 11635 (2019). PMID 31406169. [PubMed] [Article] [RRC of NBRP]