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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2alpha-S52A-PA (#RDB18640)

Expression vector of human eIF2alpha mutant (S52A) with PA-tag.

Clone info. Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha) mutant (S52A) with PA-tag at C-terminus cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human EIF2S1 cDNA
Depositor|Developer Ito, Takuhiro |
 
Sequence (partial) RDB18640pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18640 pEBMulti-Neo-human-eIF2alpha-S52A-PA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEBMulti-Neo-human-eIF2alpha-S52A-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18640).

Reference section:

Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18640_B0L8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 (Pr0495)
Region: CAG pro, insert 5' (S52A)
Sequence file: RDB18640_B0L8a.seq check
>D07570A2_B0L8_1w_pCAG-F_V3_F10_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNAGGGCG GGGTTCGGCT TCTGGCGTGT
   61 GANCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
  121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TAACCCTCAC
  181 TAAAGGGGTA CCGGGCCCCC CCTCGAGGTC GACGCCACCA TGCCGGGTCT AAGTTGTAGA
  241 TTTTATCAAC ACAAATTTCC TGAGGTGGAA GATGTAGTGA TGGTGAATGT CAGATCCATT
  301 GCTGAAATGG GGGCTTATGT CAGCTTGCTG GAATACAACA ACATTGAAGG CATGATTCTT
  361 CTTAGTGAAT TAGCCAGAAG GCGTATCCGT TCTATCAACA AACTCATCCG AATTGGCAGG
  421 AATGAGTGTG TGGTTGTCAT TAGGGTGGAC AAAGAAAAAG GATATATTGA TTTGTCAAAA
  481 AGAAGAGTTT CTCCAGAGGA AGCAATCAAA TGTGAAGACA AATTCACAAA ATCCAAAACT
  541 GTTTATAGCA TTCTTCGTCA TGTTGCTGAG GTGTTAGAAT ACACCAAGGA TGAGCAGCTG
  601 GAAAGCCTAT TCCAGAGGAC TGCCTGGGTC TTTGATGACA AGTACAAGAG ACCTGGATAT
  661 GGTGCCTATG ATGCATTTAA GCATGCAGTC TCAGACCCAT CTATTTTGGA TAGTTTAGAT
  721 TTGAATGAAG ATGAACGGGA AGTACTCATT AATAATATTA ATAGGCGCTT GACCCCACAG
  781 GCTGTCNAAA TTCNAGCAGA TATTGNAGTG GCTTGTTATG GTTATGAANG CNTTGANGCT
  841 GTAAAAGAAN NCCTAANANC NNGNTTGAAT TGNTCTACAG AAAANNTGNC CATTAAGATT
  901 AATCNNANAN CTNCTCCTCN GTATGTAATG ACTACNANNN NCCTGGANAN AACANANNGN
  961 CTTTCTGTCN TCNNTCNANN NNATGGNTGN TATCNAANAN AANNNNTGNN GNNANANNNG
 1021 GGTGNGNNNN NNGNTNNANT NNNNNNCCNA ANGNNNNNNC NNANNNCNGA NGANANNNNN
1081 CNTNNNNNNN CNNNNNNNNN N
//
Primer: pAxEF R2 (Pr0059)
Region: insert 3'
Sequence file: RDB18640_B0L8b.seq check
>D07570A2_B0L8_1_pAxEF R2_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NTTGTNNATT TGTGANGCTA TTNNCTTTAT TTGTAACCAT
   61 TATAANNCTG CAATAAACNN NTNAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
  121 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  181 ATTATGATCT AGAGTCGCGG CCGCTTACAC CACATCATCT TCGGCACCTG GCATGGCAAC
  241 GCCATCTTCA GCTTTGGCTT CCATTTCTTC TGCATCATCA TCTCCATCCA CTTCGGCATT
  301 TTCTCTTTCA AGCCTCTCCA TCTGCCTCGC AAGTTCAGTC TCATCTGTAT CTGTGACCAC
  361 TTTGGGCTCC ATTTGAACAT TGAACACACC CCTCTTTTCC TCAATCTTCT CTTTGATAAC
  421 AGCCATAGCT TGACTGAGGA CAGAAAGGCC TTCTGTTCTC TCCAGGGTTG TCGTAGTCAT
  481 TACATACCGA GGAGGAGCTA TTAGATTAAT CTTAATGGGC ATGTTTTCTG TAGAACAATT
  541 CAAACCTGCT CTTAGGGCTT CTTTTACAGC ATCAATGCCT TCATAACCAT AACAAGCCAC
  601 TTCAATATCT GCTCGAATTT TGACAGCCTG TGGGGTCAAG CGCCTATTAA TATTATTAAT
  661 GAGTACTTCC CGTTCATCTT CATTCAAATC TAAACTATCC AAAATAGATG GGTCTGANAC
  721 TGCATGCTTA AATGCATCAT ANGCACCATA TCCAGGTCTC TTGTACTTGT CATCAAAGAN
  781 CCANGCAGTC CTCTNGAATA GGCTTTCNNG CTGCTCATCC TTGGNGNANT CTAACACCTC
  841 ANCNNCATGN NNAANAATGC TANAAACNGT TTTGNANTTT GTGAATTTGN CTNCNCATTT
  901 GNNTGCTNNN NNNNNNAAAN TCNNCTTTTT NANNNNNNNN ANNTNCNNTT TTTNNNTNNN
961 NNNNNCNNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18640_B0L8c.seq check
>D07570A2_B0L8_1_SV40pro_ori_F_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCC TCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAGATCATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAANAGC
  601 ATCANGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
  661 ANGNNCTCGT CGTGANCCAT GGCGATGCCT GCTTGCCNAA TATCATGGTG GAAAATGGCC
  721 GCTNTTCTGG ATTCATCGAC TGTGGNCGGN TGNNTGTGGC GGANCGCTAT CANGANATAN
  781 CGTNNGCTAC CNGNGANATT GCTGNANANC TTNNNNGCNA ANGGGNTGAN CGCTTNCNCN
841 NGNNTTNNNN NATCNNNNCT CCNNANNCNN NNNNNANNNN NNTNNNNNNN NNN
//
Primer: oriP_F (Pr0765)
Region: oriP, EBNA-1
Sequence file: RDB18640_B0L8d.seq check
>D07570A2_B0L8_1w_oriP_F_G10_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGGCGNNNNN NTCGCTGCTT GTCCTTTTCC
   61 TGCTGGTTGC TCCCATTCTT AGGTGAATTT AAGGAGGCCA GGCTAAAGCC GTCGCATGTC
  121 TGATTGCTCA CCAGGTAAAT GTCGCTAATG TTTTCCAACG CGAGAAGGTG TTGAGCGCGG
  181 AGCTGAGTGA CGTGACAACA TGGGTATGCC CAATTGCCCC ATGTTGGGAG GACGAAAATG
  241 GTGACAAGAC AGATGGCCAG AAATACACCA ACAGCACGCA TGATGTCTAC TGGGGATTTA
  301 TTCTTTAGTG CGGGGGAATA CACGGCTTTT AATACGATTG AGGGCGTCTC CTAACAAGTT
  361 ACATCACTCC TGCCCTTCCT CACCCTCATC TCCATCACCT CCTTCATCTC CGTCATCTCC
  421 GTCATCACCC TCCGCGGCAG CCCCTTCCAC CATAGGTGGA AACCAGGGAG GCAAATCTAC
  481 TCCATCGTCA AAGCTGCACA CAGTCACCCT GATATTGCAG GTAGGAGCGG GCTTTGTCAT
  541 AACAAGGTCC TTAATCGCAT CCTTCAAAAC CTCAGCAAAT ATATGAGTTT GTAAAAAGAC
  601 CATGAAATAA CAGACAATGG ACTCCCTTAG CGGGCCAGGT TGTGGGCCGG GTCCAGGGGC
  661 CATTCCAAAG GGGAGACGAC TCAATGGTGT AAGACGACAT TGTGGAATAG CAAGGGCAGT
  721 TCCTCGCCTT AGGTTGTAAA GGGAGGTCTT ACTACCTCCA TATACNAACA CACCGGCGAC
  781 CCAAGTTCCT TCGTCGGTAG TNCTTTCTAC GTGACTCCTA NCCAGGAGAG CTCNTAAACC
  841 TTCTGNAATG TTCTCNAATT TCNGGTTGNA ANCTCNTTGA CCNCNATGCT TTCCAAANCA
  901 CCCTCCTTTT TTGNNNCTGN CTNNNTCACC CTGNNCCCGG GGNNCNGTGN TNGGGCCTTC
  961 TCCTGGGTCA TCTGNNGGGC CNNGCTCTAN NNNTNCCNGG GGNNNNNNCA NNNTCNNCAT
 1021 CTGGGNNNNN TTCTTGGNGG TANTCNAAAN NATNNGNTTC CCNNNNNNGN NNGAAAAATG
1081 GNNNTNNNAN NNNNNNNGGG GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP]