Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2alpha-S52A-PA

Expression vector of human eIF2Balpha mutant (S52A) with PA-tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18640_B0L8p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3 Region: CAG pro, insert 5' (S52A) Sequence file: RDB18640_B0L8a.seq
>D07570A2_B0L8_1w_pCAG-F_V3_F10_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNAGGGCG GGGTTCGGCT TCTGGCGTGT    61 GANCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT   121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TAACCCTCAC   181 TAAAGGGGTA CCGGGCCCCC CCTCGAGGTC GACGCCACCA TGCCGGGTCT AAGTTGTAGA   241 TTTTATCAAC ACAAATTTCC TGAGGTGGAA GATGTAGTGA TGGTGAATGT CAGATCCATT   301 GCTGAAATGG GGGCTTATGT CAGCTTGCTG GAATACAACA ACATTGAAGG CATGATTCTT   361 CTTAGTGAAT TAGCCAGAAG GCGTATCCGT TCTATCAACA AACTCATCCG AATTGGCAGG   421 AATGAGTGTG TGGTTGTCAT TAGGGTGGAC AAAGAAAAAG GATATATTGA TTTGTCAAAA   481 AGAAGAGTTT CTCCAGAGGA AGCAATCAAA TGTGAAGACA AATTCACAAA ATCCAAAACT   541 GTTTATAGCA TTCTTCGTCA TGTTGCTGAG GTGTTAGAAT ACACCAAGGA TGAGCAGCTG   601 GAAAGCCTAT TCCAGAGGAC TGCCTGGGTC TTTGATGACA AGTACAAGAG ACCTGGATAT   661 GGTGCCTATG ATGCATTTAA GCATGCAGTC TCAGACCCAT CTATTTTGGA TAGTTTAGAT   721 TTGAATGAAG ATGAACGGGA AGTACTCATT AATAATATTA ATAGGCGCTT GACCCCACAG   781 GCTGTCNAAA TTCNAGCAGA TATTGNAGTG GCTTGTTATG GTTATGAANG CNTTGANGCT   841 GTAAAAGAAN NCCTAANANC NNGNTTGAAT TGNTCTACAG AAAANNTGNC CATTAAGATT   901 AATCNNANAN CTNCTCCTCN GTATGTAATG ACTACNANNN NCCTGGANAN AACANANNGN   961 CTTTCTGTCN TCNNTCNANN NNATGGNTGN TATCNAANAN AANNNNTGNN GNNANANNNG  1021 GGTGNGNNNN NNGNTNNANT NNNNNNCCNA ANGNNNNNNC NNANNNCNGA NGANANNNNN 1081 CNTNNNNNNN CNNNNNNNNN N //
Primer: pAxEF R2 Region: insert 3' Sequence file: RDB18640_B0L8b.seq
>D07570A2_B0L8_1_pAxEF R2_F08_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN NTTGTNNATT TGTGANGCTA TTNNCTTTAT TTGTAACCAT    61 TATAANNCTG CAATAAACNN NTNAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC   121 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG   181 ATTATGATCT AGAGTCGCGG CCGCTTACAC CACATCATCT TCGGCACCTG GCATGGCAAC   241 GCCATCTTCA GCTTTGGCTT CCATTTCTTC TGCATCATCA TCTCCATCCA CTTCGGCATT   301 TTCTCTTTCA AGCCTCTCCA TCTGCCTCGC AAGTTCAGTC TCATCTGTAT CTGTGACCAC   361 TTTGGGCTCC ATTTGAACAT TGAACACACC CCTCTTTTCC TCAATCTTCT CTTTGATAAC   421 AGCCATAGCT TGACTGAGGA CAGAAAGGCC TTCTGTTCTC TCCAGGGTTG TCGTAGTCAT   481 TACATACCGA GGAGGAGCTA TTAGATTAAT CTTAATGGGC ATGTTTTCTG TAGAACAATT   541 CAAACCTGCT CTTAGGGCTT CTTTTACAGC ATCAATGCCT TCATAACCAT AACAAGCCAC   601 TTCAATATCT GCTCGAATTT TGACAGCCTG TGGGGTCAAG CGCCTATTAA TATTATTAAT   661 GAGTACTTCC CGTTCATCTT CATTCAAATC TAAACTATCC AAAATAGATG GGTCTGANAC   721 TGCATGCTTA AATGCATCAT ANGCACCATA TCCAGGTCTC TTGTACTTGT CATCAAAGAN   781 CCANGCAGTC CTCTNGAATA GGCTTTCNNG CTGCTCATCC TTGGNGNANT CTAACACCTC   841 ANCNNCATGN NNAANAATGC TANAAACNGT TTTGNANTTT GTGAATTTGN CTNCNCATTT   901 GNNTGCTNNN NNNNNNAAAN TCNNCTTTTT NANNNNNNNN ANNTNCNNTT TTTNNNTNNN 961 NNNNNCNNNN NNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB18640_B0L8c.seq
>D07570A2_B0L8_1_SV40pro_ori_F_G08_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCC TCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGAGGCTTTT    61 TTGGAGGCCT AGGCTTTTGC AAAGATCATC AAGAGACAGG ATGAGGATCG TTTCGCATGA   121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT   181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC   241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG   301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG   361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC   421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC   481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG   541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAANAGC   601 ATCANGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG   661 ANGNNCTCGT CGTGANCCAT GGCGATGCCT GCTTGCCNAA TATCATGGTG GAAAATGGCC   721 GCTNTTCTGG ATTCATCGAC TGTGGNCGGN TGNNTGTGGC GGANCGCTAT CANGANATAN   781 CGTNNGCTAC CNGNGANATT GCTGNANANC TTNNNNGCNA ANGGGNTGAN CGCTTNCNCN 841 NGNNTTNNNN NATCNNNNCT CCNNANNCNN NNNNNANNNN NNTNNNNNNN NNN //
Primer: oriP_F Region: oriP, EBNA-1 Sequence file: RDB18640_B0L8d.seq
>D07570A2_B0L8_1w_oriP_F_G10_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGGCGNNNNN NTCGCTGCTT GTCCTTTTCC    61 TGCTGGTTGC TCCCATTCTT AGGTGAATTT AAGGAGGCCA GGCTAAAGCC GTCGCATGTC   121 TGATTGCTCA CCAGGTAAAT GTCGCTAATG TTTTCCAACG CGAGAAGGTG TTGAGCGCGG   181 AGCTGAGTGA CGTGACAACA TGGGTATGCC CAATTGCCCC ATGTTGGGAG GACGAAAATG   241 GTGACAAGAC AGATGGCCAG AAATACACCA ACAGCACGCA TGATGTCTAC TGGGGATTTA   301 TTCTTTAGTG CGGGGGAATA CACGGCTTTT AATACGATTG AGGGCGTCTC CTAACAAGTT   361 ACATCACTCC TGCCCTTCCT CACCCTCATC TCCATCACCT CCTTCATCTC CGTCATCTCC   421 GTCATCACCC TCCGCGGCAG CCCCTTCCAC CATAGGTGGA AACCAGGGAG GCAAATCTAC   481 TCCATCGTCA AAGCTGCACA CAGTCACCCT GATATTGCAG GTAGGAGCGG GCTTTGTCAT   541 AACAAGGTCC TTAATCGCAT CCTTCAAAAC CTCAGCAAAT ATATGAGTTT GTAAAAAGAC   601 CATGAAATAA CAGACAATGG ACTCCCTTAG CGGGCCAGGT TGTGGGCCGG GTCCAGGGGC   661 CATTCCAAAG GGGAGACGAC TCAATGGTGT AAGACGACAT TGTGGAATAG CAAGGGCAGT   721 TCCTCGCCTT AGGTTGTAAA GGGAGGTCTT ACTACCTCCA TATACNAACA CACCGGCGAC   781 CCAAGTTCCT TCGTCGGTAG TNCTTTCTAC GTGACTCCTA NCCAGGAGAG CTCNTAAACC   841 TTCTGNAATG TTCTCNAATT TCNGGTTGNA ANCTCNTTGA CCNCNATGCT TTCCAAANCA   901 CCCTCCTTTT TTGNNNCTGN CTNNNTCACC CTGNNCCCGG GGNNCNGTGN TNGGGCCTTC   961 TCCTGGGTCA TCTGNNGGGC CNNGCTCTAN NNNTNCCNGG GGNNNNNNCA NNNTCNNCAT  1021 CTGGGNNNNN TTCTTGGNGG TANTCNAAAN NATNNGNTTC CCNNNNNNGN NNGAAAAATG 1081 GNNNTNNNAN NNNNNNNGGG GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.27

GNP_filter3_RDBDEP_html_220727.pl