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pYC135 pBS-GPDp-Ifa38-V5-GFP11-CyC1ter-natNT2 (#RDB18656)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: endoplasmic reticulum.

Alternative name pYC135, pBS-GPDp-Ifa38-V5-GFP11-CyC1ter-natNT2
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, natNT2 (clonNAT resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Nourseothricin (yeast).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB18656zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18656 pYC135 pBS-GPDp-Ifa38-V5-GFP11-CyC1ter-natNT2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYC135 pBS-GPDp-Ifa38-V5-GFP11-CyC1ter-natNT2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18656).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18656_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308)
Region: f1 ori, T7 pro, insert 5'(GPD pro)
Sequence file: RDB18656_B0Lna.seq check
>D07536A2_B0Ln_1_f1ori_F2_A10_01_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNNTT TNNCGANTTC GNNCTATTGG TTAAAAAAAT GAGCTGATTT
   61 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC ATTCGCCATT
  121 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT
  181 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC
  241 ACGACGTTGT AAAACGACGG CCAGTGAGCG CGCGTAATAC GACTCACTAT AGGGCGAATT
  301 GGGTACCGGG CCCCCCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTAGTTTA
  361 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
  421 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
  481 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
  541 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
  601 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
  661 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
  721 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
  781 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA
  841 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGANGG
  901 NAGGTATTGA TTGNAATTCT GTAAATCTAT TTCNNAAACT NCTNNAATNN NACTTTTANA
  961 GTNAGTCTTT TTTTTTANTT TTAAAACACN NNAACTTNNT TNNNNNNNAN GNNGNNNNNN
1021 NNNN
//
Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: T3 pro, insert 3'(TEF pro, natNT2 5')
Sequence file: RDB18656_B0Lnb.seq check
>D07536A2_B0Ln_1_pUC-CAPbs_up_F2_B10_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNCTTTATGC TTCCGGCTCG TATGTTGTGT
   61 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG
  121 CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC
  181 TCTAGAACTA GTGGATCCCG GGTTAATTAA GGCGCGCCAG ATCTGTTTAG CTTGCCTCGT
  241 CCCCGCCGGG TCACCCGGCC AGCGACATGG AGGCCCAGAA TACCCTCCTT GACAGTCTTG
  301 ACGTGCGCAG CTCAGGGGCA TGATGTGACT GTCGCCCGTA CATTTAGCCC ATACATCCCC
  361 ATGTATAATC ATTTGCATCC ATACATTTTG ATGGCCGCAC GGCGCGAAGC AAAAATTACG
  421 GCTCCTCGCT GCAGACCTGC GAGCAGGGAA ACGCTCCCCT CACAGACGCG TTGAATTGTC
  481 CCCACGCCGC GCCCCTGTAG AGAAATATAA AAGGTTAGGA TTTGCCACTG AGGTTCTTCT
  541 TTCATATACT TCCTTTTAAA ATCTTGCTAG GATACAGTTC TCACATCACA TCCGAACATA
  601 AACAACCATG GGTACCACTC TTGACGACAC GGCTTACCGG TACCGCACCA GTGTCCCGGG
  661 GGACGCCGAG GCCATCGAGG CACTGGATGG GTCCTTCACC ACCGACACCG TCTTCCGCGT
  721 CACCGCCACC GGGGACGGCT TCACCCTGCG GGANGTGCCG GTGGACCCGC CCCTGACCAA
  781 NGTGTTCCCC GACGACNAAT CGGACGANGA ATCGGANGAN GGGGAGGANG GCGACCCGGA
  841 NTCNCGGANG TTCGTCGCGT ACGGGGANNA NGGCGANCTG GCGGGCTTCG TGGNCGTCTC
  901 GNACTCCGGC NNGAANCGCC NGCTGANCGN CNANGANNTC GANGNCGCCC CGGANCACCG
  961 GGGGCNCGGG NNNGGGNGCG CGNNGANGGG NNNNGCGANN NANNNNGNCN NCNANNNGGN
1021 NNNNNGNNNN NNNNNGNNNN NNNNNN
//
Primer: TDH3pro_F (Pr0431)
Region: insert mid(GPD pro, Ifa38 5')
Sequence file: RDB18656_B0Lnc.seq check
>D07536A2_B0Ln_1_TDH3pro_F_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNT GNNNTTCTNN NNNNTATTTC TTAAACTTCT TAAATTCTAC
   61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG
  121 CCGCATGACT TTTATGCAAC AGCTTCAAGA GGCTGGGGAA AGATTTAGGT GTATCAATGG
  181 TCTTTTATGG GTTGTTTTTG GGCTTGGTGT CTTAAAATGT ACAACGTTAT CACTTAGATT
  241 TTTAGCTCTT ATTTTTGATC TTTTTTTACT ACCAGCAGTC AATTTCGACA AGTATGGTGC
  301 TAAAACTGGT AAATACTGTG CTATCACTGG TGCAAGTGAC GGTATTGGTA AAGAATTTGC
  361 TAGACAAATG GCGAAACGTG GCTTCAACTT GGTATTGATC TCGAGAACGC AATCTAAATT
  421 GGAGGCTTTA CAAAAAGAAC TAGAAGATCA ACATCATGTC GTTGTAAAGA TTCTAGCCAT
  481 TGACATTGCG GAGGATAAAG AATCCAACTA TGAATCCATT AAGGAATTGT GTGCGCAGTT
  541 ACCAATCACC GTTTTGGTCA ATAATGTTGG TCAATCACAC TCCATTCCTG TTCCATTTTT
  601 GGAAACAGAA GAGAAGGAGC TTAGAAATAT TATCACTATC AATAACACTG CTACATTATT
  661 AATTACACAA ATCATTGCAC CAAAGATTGT GGAAACTGTG AAAGCTGAAA ACAAGAAGTC
  721 GGGTACTCGT GGTTTGATCT TAACCATGGG CTCATTTGGT GGTCTGATTC CCACCCCACT
  781 TTTGGCTACA TACAGTGGTT CGAAATCATT CTTACAAGGT TGGTCTAACT CTTTGGCTGG
  841 GGANTTATCT AAAGATGCTA TCGATGTTGA ATTAATCATT TCATATTNGG TCANTAGCTC
  901 GATGTCTAAA ATCAGAAGAN CATNTTTAAT GATCCNNANT CCNCANCAGT TTGTAAAATC
  961 CNCTTNNNNN NNNGNNNGNN GNNGCNNCNN NNNNCNNNAA ANNNNNNCTN NNNNNNNCNN
1021 NTNNNNNGNN NNNNNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid(CYC1 ter, GFP11, V5 tag, Ifa38 3')
Sequence file: RDB18656_B0Lnd.seq check
>D07536A2_B0Ln_1_CYC1ter_R_D10_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NGGGGGGANG GCGTGAATGT AAGCGTGACA
   61 TAACTAATTA CATGACTCGA GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCTTAGGT
  121 AATGCCTGCA GCATTGACAT ATTCGTGTAG AACCATATGA TCTCTGCTTG TGGAACCACC
  181 ACCACTTCCA CCAGTTGAGT CTAAGCCCAA AAGAGGGTTT GGTATGGGTT TCCCAGAACC
  241 TCCGGCAGAG GATCCTTCCT TTTTAACCTG TCTTGCGGCT TTTTTTAAGG CTCTAATTCT
  301 GATAGATTTA TGGAAGGAAT AATTAATGGA GTTAACAATC TTAGAGTAAA CGCCAAAGGT
  361 CTCTGTAATT ACAAATTGAT ACACTGCGTG CGCCCAGTAA GGGGTCATAG TAGCGTATCT
  421 TTCTTGAGAG CCGCAGCGTC TGCCAACGCT TCTTAAAGTG GATTTTACAA ACTGTTGTGG
  481 ATTTGGGATC ATTAAAGATG ATCTTCTGAT TTTAGACATC GAGCTAGTGA CCAAATATGA
  541 AATGATTAAT TCAACATCGA TAGCATCTTT AGATAATTCC CCAGCCAAAG AGTTAGACCA
  601 ACCTTGTAAG AATGATTTCG AACCACTGTA TGTAGCCAAA AGTGGGGTGG GAATCAGACC
  661 ACCAAATGAG CCCATGGTTA AGATCAAACC ACGAGTACCC GACTTCTTGT TTTCAGCTTT
  721 CACAGTTTCC ACAATCTTTG GTGCAATGAT TTGTGTAATT AATAATGTAG CAGTGTTATT
  781 GATAGTGATA ATATTTCTAA GCTCCTTCTC TTCTGTTTCC NAAAATGGAA CAGGAATGGA
  841 GTGTGANTGA NCANCATTAT TGACCAAACG GTGATTGGTA ACTGCGCACA CAATTCCNTT
  901 AANGGANTCN TAGTTNGGAN NNTTTATCCT CCGCANTGTC NANGGNTANA ATCTTNNNNN
  961 NGACNTGATG TTGNNCTTCT ANTNCNTTTT NGNAAANNCN NNNNNTTTAN ANNGNNTNNN
1021 NNANNNNNNT ANNNANNNNN NNNNN
//
Primer: CYC1ter_F (Pr0273)
Region: insert mid(ADH1 ter, natNT2 3')
Sequence file: RDB18656_B0Lne.seq check
>D07536A2_B0Ln_1_CYC1ter_F_E10_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN NNNTNNNNNN NTTTAATTTG CGGCCGANCT CGATTACAAC
   61 AGGTGTTGTC CTCTGAGGAC ATAAAATACA CACCGAGATT CATCAACTCA TTGCTGGAGT
  121 TAGCATATCT ACAATTGGGT GAAATGGGGA GCGATTTGCA GGCATTTGCT CGGCATGCCG
  181 GTAGAGGTGT GGTCAATAAG AGCGACCTCA TGCTATACCT GAGAAAGCAA CCTGACCTAC
  241 AGGAAAGAGT TACTCAAGAA TAAGAATTTT CGTTTTAAAA CCTAAGAGTC ACTTTAAAAT
  301 TTGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA
  361 TTCGCCTCGA GATTAGGGGC AGGGCATGCT CATGTAGAGC GCCTGCTCGC CGTCCGAGGC
  421 GGTGCCGTCG TACAGGGCGG TGTCCAGGCC GCAGAGGGTG AACCCCATCC GCCGGTACGC
  481 GTGGATCGCC GGTGCGTTGA CGTTGGTGAC CTCCAGCCAG AGGTGCCCGG CGCCCCGCTC
  541 GCGGGCGAAC TCCGTCGCGA GCCCCATCAA CGCGCGCCCG ACCCCGTGCC CCCGGTGCTC
  601 CGGGGCGACC TCGATGTCCT CGACGGTCAG CCGGCGGTTC CAGCCGGAGT ACGAGACGAC
  661 CACGAAGCCC GCCAGGTCGC CGTCGTCCCC GTACGCGACG AACGTCCGGG AGTCCGGGTC
  721 GCCGTCCTCC CCGTCGTCCG ATTCGTCGTC CGATTCGTCG TCNGGGAACA CCTTGGTCAG
  781 GGGCGGGTCC ACCGGCACCT CCCGCANGGT GAAGCCGTCC CCGGTGGNGG TGANGCGGAN
  841 NANGGTGTCG GNGGTGAANG ANCCATCCNG TGNCTCNATG GNCTCNGCGT CCCCCGGGAN
  901 ACTGGTGCGG TACCGGTAAG CCGNGNCGTN NNGANTGGTA CCNANNGNNN GNTTANNNNC
961 GNNNGNGATG TGANANNNGN ANNCNTANCN NGANTTTNAA NGNNNNNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]