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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2 (#RDB18657)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: lipid droplets.

Alternative name pYC136, pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, natNT2 (clonNAT resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Nourseothricin (yeast).
Gene/insert name S. cerevisiae ERG6 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB18657zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18657 pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18657).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18657_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308)
Region: f1 ori, T7 pro, GPD pro
Sequence file: RDB18657_B0Lna.seq check
>D07536A3_B0Ln_2_f1ori_F2_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTTCGNNCT NTTGNNNNNN NNAATGAGCT
   61 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTCCATTCG
  121 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
  181 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
  241 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC
  301 GAATTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC
  361 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT
  421 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC
  481 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT
  541 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA
  601 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT
  661 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCNNTG
  721 GAGTGATGCA NCCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC
  781 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGANTTGNA AAAAGCTGAA
  841 AAAAAAGGTT GAAACCNGTT CCCTGAAATT ATNCCCCTAC TTGANTAATA AGNATANNAA
  901 AGACNGNAGG NATTGATTGN ANTNNGNAAA TCTNTTNCTT AAACNTCTTA AATTCNACTT
961 TTATAGNTAN NCTTTTTTTT ANNTTNNAAA NNNNNNNNNN
//
Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: T3 pro, natNT2 (TEF pro, NrsR)
Sequence file: RDB18657_B0Lnb.seq check
>D07536A3_B0Ln_2_pUC-CAPbs_up_F2_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNTTTATG CTTCCGGCTC GTATGTTGTG
   61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA
  121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
  181 CTCTAGAACT AGTGGATCCC GGGTTAATTA AGGCGCGCCA GATCTGTTTA GCTTGCCTCG
  241 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT
  301 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC
  361 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC
  421 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT
  481 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC
  541 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT
  601 AAACAACCAT GGGTACCACT CTTGACGACA CGGCTTACCG GTACCGCACC AGTGTCCCGG
  661 GGGACGCCGA GGCCATCGAG GCACTGGATG GGTCCTTCAC CACCGACACC GTCTTCCGCG
  721 TCACCGCCAC CGGGGANGGC TTCACCCTGC GGGAGGTGNC GGTGGACCCG CCCCTGANCA
  781 AGGTGTTCCC CGACGACGAA TCGGANGANG AATCGGACGA NGGGGANGAN GGCGACNCGG
  841 ANTNCCGGAN GTNCGTCGCG TACNGGGANG ANGGCNACCT GNCGGGCTTC GTGGNCGTCT
  901 CGTACTCCGG CTGGNACCGN CGGCTGANCG TCNAGGANAT CGAGGTCGCC CCNNANCNNC
  961 NGGGGGCNNG GGGNCNGGCG CGCNTTNNNG GGGNTCGCGA NNNANNNCGN CNGCNANNGG
1021 GGCNNNNNNN NNN
//
Primer: TDH3pro_F (Pr0431)
Region: GPD pro, Erg6 5'
Sequence file: RDB18657_B0Lnc.seq check
>D07536A3_B0Ln_2_TDH3pro_F_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNTGNNNTTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT
   61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
  121 GCGGCCGCAT GAGTGAAACA GAATTGAGAA AAAGACAGGC CCAATTCACT AGGGAGTTAC
  181 ATGGTGATGA TATTGGTAAA AAGACAGGTT TGAGTGCATT GATGTCGAAG AACAACTCTG
  241 CCCAAAAGGA AGCCGTTCAG AAGTACTTGA GAAATTGGGA TGGTAGAACC GATAAAGATG
  301 CCGAAGAACG TCGTCTTGAG GATTATAATG AAGCCACACA TTCCTACTAT AACGTCGTTA
  361 CAGATTTCTA TGAATATGGT TGGGGTTCCT CTTTCCATTT CAGCAGATTT TATAAAGGTG
  421 AGAGTTTCGC TGCCTCGATA GCAAGACATG AACATTATTT AGCTTACAAG GCTGGTATTC
  481 AAAGAGGCGA TTTAGTTCTC GACGTTGGTT GTGGTGTTGG GGGCCCAGCA AGAGAGATTG
  541 CAAGATTTAC CGGTTGTAAC GTCATCGGTC TAAACAATAA CGATTACCAA ATTGCCAAGG
  601 CAAAATATTA CGCTAAAAAA TACAATTTGA GTGACCAAAT GGACTTTGTA AAGGGTGATT
  661 TCATGAAAAT GGATTTCGAA GAAAACACTT TCGACAAAGT TTATGCAATT GAGGCCACAT
  721 GTCACGCTCC AAAATTAGAA GGTGTATACA GCGAAATCTA CAAGGTTTTG AAACCGGGTG
  781 GTACCTTTGC TGTTTACNAA TGGGTAATGA CTGATAAATA TGACGAAAAC AATCCTGANC
  841 ATAGAAAGAN CGCTTATGAA ATTGANNTAN GTGATGGNAT CCCNAAGATG TTCCNNGTCG
  901 ACGNGGCTAG GAAAGCATTG AAGAACNGNG NNTTCNAANN CCTCNTNAGC GANNNNCTGN
  961 NGNANNNGNT GANGAAANNC CNNNNNNNNN CCNNNTNNNN NGNNGANNNN NNGTANGTNN
 1021 AAAACTNNNC TNANNTNNNN NNNNNTTTTN NNNNNNTNNN NNNNNNGGNN NNNNNNTTNN
1081 NNNNNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: CYC1 ter, GFP11, V5 tag, Erg6 3'
Sequence file: RDB18657_B0Lnd.seq check
>D07536A3_B0Ln_2_CYC1ter_R_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NAGNGGNNGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT
   61 AACTAATTAC ATGACTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCTTAGGTA
  121 ATGCCTGCAG CATTGACATA TTCGTGTAGA ACCATATGAT CTCTGCTTGT GGAACCACCA
  181 CCACTTCCAC CAGTTGAGTC TAAGCCCAAA AGAGGGTTTG GTATGGGTTT CCCAGAACCT
  241 CCGGCAGAGG ATCCTTGAGT TGCTTCTTGG GAAGTTTGGG AGGGGGTTTC GGCGTTTTCT
  301 GGCTTCCTAG CGACGAAAAG CATCATTGGA GTGAATAACT TGGACTTACC ACCGGCAACT
  361 AAACCAACCG CAGCATTTTC TAGAGCAGCA GTAACTTCCT TGGAACCTTC TGGGGCTAGA
  421 CCTAATTTTT CCATTACAGT AACCATTGCT GTAGTAAATT GTCTACCCAA GTAAGAAGTT
  481 CTGAAAAATG TGGCCAAATT AGCTAAGTTT TGAACGTACT TCCACTCACC AGTTAATGGG
  541 TAATACCAAG GGATTTCATC ATCATTGTCC GCCAGGTCTT CGCTAACGAG GACTTCGAAA
  601 CCACAGTTCT TCAATGCTTT CCTAGCCACG TCGACATGGA ACATCTTTGG GATACCATCA
  661 CCTAGTTCAA TTTCATAAGC GATCTTTCTA TGTTCAGGAT TGTTTTCGTC ATATTTATCA
  721 GTCATTACCC ATTCGTAAAC AGCAAAGGTA CCACCCGGTT TCAAAACCTT GTAGATTTCG
  781 CTGTATACAC CTTCTAATTT TGGAGCGTGA CATGTGGCCT CNATTGCATA AACTTTGTCG
  841 AAAGTGTTTT CTTCGAAATC CATTTTCNTG AAATCACCCT TTACAAAGTC CATTTGGTCA
  901 CTCNAATTGT ATTTTTTNAG CGNANTATTT TGCCTNGGCA ATTTNGNNNT CGNNATNGNT
  961 TANANNGATG ANNNNNCNAN NGGNAAATCT NGNNNNCNNN NNNGNNNGGC CCCNNACNCC
1021 NCNNCNNNCN NNNANNNNNN ANNNNNNNNN N
//
Primer: CYC1ter_F (Pr0273)
Region: CYC1 ter, natNT2 (ADH1 ter, NrsR)
Sequence file: RDB18657_B0Lne.seq check
>D07536A3_B0Ln_2_CYC1ter_F_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTTNAT TTGCGGCCGA NCTCGATTAC
   61 AACAGGTGTT GTCCTCTGAG GACATAAAAT ACACACCGAG ATTCATCAAC TCATTGCTGG
  121 AGTTAGCATA TCTACAATTG GGTGAAATGG GGAGCGATTT GCAGGCATTT GCTCGGCATG
  181 CCGGTAGAGG TGTGGTCAAT AAGAGCGACC TCATGCTATA CCTGAGAAAG CAACCTGACC
  241 TACAGGAAAG AGTTACTCAA GAATAAGAAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA
  301 AATTTGTATA CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG
  361 AAATTCGCCT CGAGATTAGG GGCAGGGCAT GCTCATGTAG AGCGCCTGCT CGCCGTCCGA
  421 GGCGGTGCCG TCGTACAGGG CGGTGTCCAG GCCGCAGAGG GTGAACCCCA TCCGCCGGTA
  481 CGCGTGGATC GCCGGTGCGT TGACGTTGGT GACCTCCAGC CAGAGGTGCC CGGCGCCCCG
  541 CTCGCGGGCG AACTCCGTCG CGAGCCCCAT CAACGCGCGC CCGACCCCGT GCCCCCGGTG
  601 CTCCGGGGCG ACCTCGATGT CCTCGACGGT CAGCCGGCGG TTCCAGCCGG AGTACGAGAC
  661 GACCACGAAG CCCGCCAGGT CGCCGTCGTC CCCGTACGCG ACGAACGTCC GGGAGTCCGG
  721 GTCGCCGTCC TCCCCGTCGT CCGATTCGTC GTCCGATTCG TCGTCGGGGA ACACCTTGGT
  781 CAGGGGCGGG TCCACCGGCA CCTCCCGCAN GGTGAAGNCG TCCCCGGTGG CGGTGANGCN
  841 GAANANGGTG TCGGTGGTGN AGGANCCATN NNGTGCCTNN ATGGNCTCNG CGTCCCCCNG
  901 GANACTGGNG CNGNACCNGN AAGCCGTGNC NNCNAGANTG GTNNCCANNG TTGNTTNANG
  961 TTCNGNNGNN NNGTGAGANN TGTATNNNNN NNNGATTTTN AANNNNNNTA TATGAAAGAN
1021 NAANNNNNNN NGNNNNAANN CNNNNNTNTN NNNNNNTTNN NNTNNNNGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]