RDB18659 - pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX (#RDB18659)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: endoplasmic reticulum.

Alternative name	pYC141, pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX
Clone info.	The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone	pBlueScript SK- (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Ampicillin (E. coli), Hygromycin B (yeast).
Gene/insert name	S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Tamura, Yasushi \|

Sequence (full)	RDB18659zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18659	pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18659).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18659_B0Lnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308) Region: f1 ori, T7 pro, insert 5' (GPD pro) Sequence file: RDB18659_B0Lna.seq
>D07536A5_B0Ln_2_f1ori_F2_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNTTTG NCGANTTCGN NCTATTGGTT AAAAAAATGA GCTGATTTAA 61 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTCCAT TCGCCATTCA 121 GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG 181 CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC 241 GACGTTGTAA AACGACGGCC AGTGAGCGCG CGTAATACGA CTCACTATAG GGCGAATTGG 301 GTACCGGGCC CCCCCTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTAGTTTATC 361 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA 421 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA 481 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG 541 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC 601 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG 661 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT 721 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA 781 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAA 841 GGNTGAAACC AGTTCCCTGA AATTATTCCN CTACTTGNCT AATAAGTATA TAAAGANGGN 901 ANGTATTGAT NGTAANTCTG NAAATCTATT TCTTNAACTN NNNAAANTCN ACTTTNNNAG 961 TTAGNCTTTT TTTTTANTTT TNAAANNNNN GANNNTNANT TNNNANNNNN NNNNN //
Primer: pUC-CAPbs_up_F2 (Pr0739) Region: T3 pro, insert 3' (TEF pro, HygR 5') Sequence file: RDB18659_B0Lnb.seq
>D07536A5_B0Ln_2_pUC-CAPbs_up_F2_G10_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCTTNNNN CTTNATGCTT CCGGCTCGTA TGTTGTGTGG 61 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG 121 CGCAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC 181 TAGAACTAGT GGATCCAGTC TTGACGTGCG CAGCTCAGGG GCATGATGTG ACTGTCGCCC 241 GTACATTTAG CCCATACATC CCCATGTATA ATCATTTGCA TCCATACATT TTGATGGCCG 301 CACGGCGCGA AGCAAAAATT ACGGCTCCTC GCTGCGGACC TGCGAGCAGG GAAACGCTCC 361 CCTCACAGAC GCGTTGAATT GTCCCCACGC CGCGCCCCTG TAGAGAAATA TAAAAGGTTA 421 GGATTTGCCA CTGAGGTTCT TCTTTCATAT ACTTCCTTTT AAAATCTTGC TAGGATACAG 481 TTCTCACATC ACATCCGAAC ATAAACAACC ATGGGTAAAA AGCCTGAACT CACCGCGACG 541 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG 601 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG 661 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG 721 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT 781 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGANC TGCCTGAAAC CGAACTGCNN 841 GCTGTTCTGC AGCCGGNCGC GGANGCCATG GATGCGATCG CTGCGGNCGA TCTTANCCNG 901 ANGANCGGGT NCGGNCNNTT NNNANCGCNN GGAATCGGNC AATACACTAC NTGGCGNGNN 961 TTNNTATGNN CNANTGNTGN NNCCCATGNG NATNANNGGN NANCNNNNNN GGANNNNNNC 1021 NNNNNNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18659_B0Lnc.seq
>D07536A5_B0Ln_2_TDH3pro_F_F11_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTGNAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT 61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT 121 GCGGCCGCAT GACTTTTATG CAACAGCTTC AAGAGGCTGG GGAAAGATTT AGGTGTATCA 181 ATGGTCTTTT ATGGGTTGTT TTTGGGCTTG GTGTCTTAAA ATGTACAACG TTATCACTTA 241 GATTTTTAGC TCTTATTTTT GATCTTTTTT TACTACCAGC AGTCAATTTC GACAAGTATG 301 GTGCTAAAAC TGGTAAATAC TGTGCTATCA CTGGTGCAAG TGACGGTATT GGTAAAGAAT 361 TTGCTAGACA AATGGCGAAA CGTGGCTTCA ACTTGGTATT GATCTCGAGA ACGCAATCTA 421 AATTGGAGGC TTTACAAAAA GAACTAGAAG ATCAACATCA TGTCGTTGTA AAGATTCTAG 481 CCATTGACAT TGCGGAGGAT AAAGAATCCA ACTATGAATC CATTAAGGAA TTGTGTGCGC 541 AGTTACCAAT CACCGTTTTG GTCAATAATG TTGGTCAATC ACACTCCATT CCTGTTCCAT 601 TTTTGGAAAC AGAAGAGAAG GAGCTTAGAA ATATTATCAC TATCAATAAC ACTGCTACAT 661 TATTAATTAC ACAAATCATT GCACCAAAGA TTGTGGAAAC TGTGAAAGCT GAAAACAAGA 721 AGTCGGGTAC TCGTGGTTTG ATCTTAACCA TGGGCTCATT TGGTGGTCTG ATTCCCACCC 781 CACTTTTGGC TACATACAGT GGTTCGAAAT CATTCTTACA AGGTTGGTCT AACTCTTTGG 841 CTGGNGAATT ATCTAAAGAT GCTATCGATG TTGAATTAAT CATTTCATAT TTGGTCNCTA 901 NCNCGATGNC TAAAATCNGA AGANCATCTT TNANGATCCN NAANCCACNN NANTTTGTAA 961 AATCCNNTTT NANANNCGNT GGNNNANNNN NNNNNN //
Primer: Ifa38_C-F (Pr0897) Region: insert mid (Ifa38 3', GFP1-10 5') Sequence file: RDB18659_B0Lnd.seq
>D07536A5_B0Ln_2_Ifa38_C-F_H10_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNGAGNNC TTTGGCGTTT ACTCTAANNN 61 NNGTTAACTC CNTTAATTAT TCCTTCCNTA AATCTATCAG AATTAGAGCC TTAAAAAAAG 121 CCGCAAGACA GGTTAAAAAG GAAGGATCCG GAGGGTCTGG TGGCGGATCA ATGGGTGGCA 181 CTAGTATGAG CAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG 241 ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGAGGAGA GGGTGAAGGT GATGCTACAA 301 TCGGAAAACT CACACTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGCCAA 361 CACTTGTCAC TACTCTGACC TATGGTGTTC AATGCTTTTC CCGTTATCCG GATCACATGA 421 AAAGGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA CGCACTATAT 481 CTTTCAAAGA TGACGGGAAA TACAAGACGC GTGCTGTAGT CAAGTTTGAA GGTGATACCC 541 TTGTTAATCG TATCGAGTTA AAGGGTACTG ATTTTAAAGA AGATGGAAAC ATTCTCGGAC 601 ACAAACTCGA GTACAACTTT AACTCACACA ATGTATACAT CACGGCAGAC AAACAAAAGA 661 ATGGAATCAA AGCTAACTTC ACAGTTCGCC ACAACGTTGA AGATGGTTCC GTTCNANCTA 721 GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC 781 CNTTACCTGT CGACNCNAAA CTGTCCTTTC GAAAGATCCC AACGAAAAGT NANNANNNNN 841 ATATCAAGCT TATCGATACC GTCNANNTCN ANTCATGNNA NNNANNNNNN NTCNNNGCTN 901 ACNTNNACNC CCTCCCCCCC NCATCCNCTC TNNNCGAAAA AGGNANNGAN NTNNNNNNAC 961 CTGNAAGTCT ANGTCCNNNT TTATTTTTTA NANNNNNNGT NNNNNNNNAG ANNNNNTATT 1021 NNNATTTNNA ANTTNNNNTT TTTTTNNNNN NNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP1-10 3') Sequence file: RDB18659_B0Lne.seq
>D07536A5_B0Ln_2_CYC1ter_R_G11_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN AGCGGNNGTG GGGGGNNGGC GTGAATGTAA GCGTGACATA 61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTACTTTT 121 CGTTGGGATC TTTCGAAAGG ACAGTTTGTG TCGACAGGTA ATGGTTGTCT GGTAAAAGGA 181 CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA ACGGAACCAT 241 CTTCAACGTT GTGGCGAACT GTGAAGTTAG CTTTGATTCC ATTCTTTTGT TTGTCTGCCG 301 TGATGTATAC ATTGTGTGAG TTAAAGTTGT ACTCGAGTTT GTGTCCGAGA ATGTTTCCAT 361 CTTCTTTAAA ATCAGTACCC TTTAACTCGA TACGATTAAC AAGGGTATCA CCTTCAAACT 421 TGACTACAGC ACGCGTCTTG TATTTCCCGT CATCTTTGAA AGATATAGTG CGTTCCTGTA 481 CATAACCTTC GGGCATGGCA CTCTTGAAAA AGTCATGCCT TTTCATGTGA TCCGGATAAC 541 GGGAAAAGCA TTGAACACCA TAGGTCAGAG TAGTGACAAG TGTTGGCCAT GGAACAGGTA 601 GTTTTCCAGT AGTGCAAATA AATTTAAGTG TGAGTTTTCC GANTGTAGCA TCACCTTCAC 661 CCTCTCCTCT GACAGAAAAT TTGTGNCCAT TAACATCACC ATCTAATTCN ACAAGAATTG 721 GGNACAACTC CNGTGAAAAG TTCTTCTCCT TTGCTCNTAC TANTGCCACC CATTGATCCN 781 CCACCNGANC CTCCGGANCC TTCCTTTTTN ACCTGTCTTG CGGNTTTTTT TANNNTCTNN 841 TTCTGANANA TTTATGGNAN GGAANNATTN NNGNANNTNA NNNTCTNANA NNAAANGCCN 901 NNGGTCTCTG NAANTNNNAN NTGANANNNN NNNNGNNNCC NNNNNNGNNN NNNNNNN //
Primer: CYC1ter_F (Pr0273) Region: insert mid(HygR) Sequence file: RDB18659_B0Lnf.seq
>D07536A5_B0Ln_2_CYC1ter_F_H11_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NGCTCNNNNN NTNNNNNNTG NNGGCCGGGC AGATGATGTC 61 GAGGCGAAAA AAAATATANN TCACGCTAAC ATTTGATTAA AATAGAACAA CTACAATATA 121 AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT CAGTACTGAT 181 TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT 241 ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC 301 CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA 361 TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG 421 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC 481 ATACAAGCCA ACCACGGCCT CCAGAAGAAG ATGTTGGCGA CCTCGTATTG GGAATCCCCG 541 AACATCGCCT CGCTCCAGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CAGGACATTG 601 TTGGAGCCGA AATCCGCGTG CACGAGGTGC CGGACTTCGG GGCAGTCCTC GGCCCAAAGC 661 ATCAGCTCAT CGAGAGCCTG CGCGACGGAC GCACTGACGG TGTCGTCCAT CACAGTTTGC 721 CAGTGATACA CATGGGGATC AGCAATCGCG CATATGAAAT CACGCCATGT AGTGTATTGA 781 CCGATTCCTT GCGGTCCGAA TGGGNCGAAC CCGCTCGTCT GGCTAANATC GGNCGCANCG 841 ATCGCATCCA TGGCCTCCGC GACCGGCTGC AGAACAGNNG GCAGTNCNGT TNCNNGCNNN 901 CTTGCAACGN GACNCCCTGN GCNNGGNGGG AGANGCAATN NNCANGNTCT NGCTNAANNT 961 CCCCNANGNC NAGCACTTCC GGAATCNGGN NCGCGGNCNN ANNCAAANNG NNNNNAAACN 1021 TANNNATNNT NGNANNNNNC NNNNNGNNNN NNNNNN //
Primer: GFP-C (Pr0304) Region: insert mid(CYC1 ter,Hygr 3') Sequence file: RDB18659_B0Lng.seq
>D07536A5_B0Ln_2_GFP-C_F04_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTGTCCTTTC NANNNANCCC AACGAANANN 61 ANGAATTCGA TATCAAGCTT ATCGATACCG TCGACCTCGA GTCATGTAAT TAGTTATGTC 121 ACGCTTACAT TCACGCCCTC CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA 181 ACCTGAAGTC TAGGTCCCTA TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT 241 TATATTTCAA ATTTTTCTTT TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT 301 GAAAACCTTG CTTGAGAAGG TTTTGGGACG CTCGAAGGCT TTAATTTGCG GCCGGGCAGA 361 TGATGTCGAG GCGAAAAAAA ATATAAATCA CGCTAACATT TGATTAAAAT AGAACAACTA 421 CAATATAAAA AAACTATACA AATGACAAGT TCTTGAAAAC AAGAATCTTT TTATTGTCAG 481 TACTGATTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG 541 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC 601 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CCCTGCGCCC AAGCTGCATC 661 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCNATGC GGAGCATATA 721 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CNCTCGAAGT ANCGCGTCTG 781 CTGCTCCATA CAAGCCNNCC ACGGNCTCCA GAAGAAGANG NTGGCGANCT CNTATTGGGA 841 ATCCCCGAAC ATCGCCTCNC TNNAGTCAAT GACCGCNGTN NTGCGGCCAT TGTCCGTCAG 901 GACATTGTTG GANCCNAAAT CCNCGNGNNC GANGNGNNNG NANTTCNGGG CNNTCNTCNN 961 NCCNNANNNN NNNNNTCATN NANAGNCTGN GCNANNNANG CACNGNNNGT GNNNTCCNNC 1021 ANAGNTTNGN CAGNGNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]