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pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX (#RDB18660)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: mitochondria.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Tashiro, S., Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pYC143, pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (TOM71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin B (yeast).
Growth conditions LB+Ampicillin, 37oC
Gene/insert name S. cerevisiae TOM71 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog # Resource name Shipping form Fee
RDB18660 pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18660).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [PubMed] [Article] [RRC of NBRP]