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pX330 gIsl1

Cas9/guideRNA expression vector for cutting ISL1 gene.

Catalog number RDB18671
Resource name pX330 gIsl1
Clone info. Cas9/guideRNA expression vector for cutting ISL1 gene. For more information, See Tsukamoto, S. et al., Stem Cell Res., 53: 102363, 2021.
Vector backbone pX330 (plasmid)
Size of vector backbone 4.3 kb
Gene/insert name human ISL1 synthetic gRNA.
Depositor|Developer Nakade, Koji |
Other clones in our bank
External Database
human ISL1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
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          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tsukamoto, S. et al., Stem Cell Research, 53, 102363, 2021)。
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          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

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          RDB18671 pX330 gIsl1 Under QC test. Please contact us. DNA solution

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          How to cite this biological resource

          Materials & Methods section:

          The pX330 gIsl1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18671).

          Reference section:

          Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., Hayashi, Y., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Tsukamoto, S., Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing. Stem Cell Res. 53: 102363 (2021). PMID 34087992. [link to RRC of NBRP]
          reference Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [link to RRC of NBRP]

          2024.04.04

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