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pcDNA3.1-FLAG-hAR

Expression vector of human androgen receptor (AR) tagged with FLAG, CMV promoter.

Catalog number RDB18676
Resource name pcDNA3.1-FLAG-hAR
Clone info. Expression vector of human androgen receptor (AR) tagged with FLAG, CMV promoter.
Comment This clone contains a cDNA fragment corresponding to the 1130nt to 3889nt of NM_000044.6. Known differences (at least) : 8 polyglutamine tract instead of 23 polyglutamine tract after 1298nt.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human AR cDNA
Depositor|Developer Negishi, Masahiko |
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External Database
human AR

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
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          提供条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
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          RDB18676 pcDNA3.1-FLAG-hAR DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1-FLAG-hAR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18676).

          Reference section:

          Shizu, R., Yokobori, K., Perera, L., Pedersen, L., Negishi, M., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18676_B1Aqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: T7 pro,insert 5'(FLAG,AR 5')
          Sequence file: RDB18676_B1Aqa.seq check
          >18676_18676_B1Aq_2_CMV-Forward_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCN NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 NNNNNNNNNT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGGCTAGTTA AGCTTGGTAC CGAGCTCGGA TCCACTAGTC
            181 CAGTGTGGTG GAATTGCCCT AAGGGCAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCC
            241 ATGGACTACA AGGATGACGA TGACAAGGCA GCTGAGCAGA AACTCATCTC TGAAGAGGAT
            301 CTGACTAGTG GAGAAGTGCA GTTAGGGCTG GGAAGGGTCT ACCCTCGGCC GCCGTCCAAG
            361 ACCTACCGAG GAGCTTTCCA GAATCTGTTC CAGAGCGTGC GCGAAGTGAT CCAGAACCCG
            421 GGCCCCAGGC ACCCAGAGGC CGCGAGCGCA GCACCTCCCG GCGCCAGTTT GCTGCTGCTG
            481 CAGCAGCAGC AGCAGCAGCA GCAAGAGACT AGCCCCAGGC AGCAGCAGCA GCAGCAGGGT
            541 GAGGATGGTT CTCCCCAAGC CCATCGTAGA GGCCCCACAG GCTACCTGGT CCTGGATGAG
            601 GAACAGCAAC CTTCACAGCC GCAGTCGGCC CTGGAGTGCC ACCCCGAGAG AGGTTGCGTC
            661 CCAGAGCCTG GAGCCGCCGT GGCCGCCAGC AAGGGGCTGC CGCAGCAGCT GCCAGCACCT
            721 CCGGACGAGG ATGACTCAGC TGCCCCATCC ACGTTGTCCC TGCTGGGCCC CACTTTCCCC
            781 GGCTTAAGCA GCTGCTCCGC TGANCTTAAA GACATCCTGA NCGAGGCCAG CACCATGCAA
            841 CTCCTTCANN AACANCAGCA NGAANCANTA TCCGAANGCA NCANCNNNNG GNNANCNNNG
            901 GANGNNTNNN GGGNCTCCCA NTTNNNCCNN NNACNNNTAC NTANGGGGCN CTTCNANNNT
          961 TTNNGANNNN NNCNNNNNNN NNGNNNNANN NNNNNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: insert 3'(AR 3')
          Sequence file: RDB18676_B1Aqb.seq check
          >18676_18676_B1Aq_2_bGH_rev3_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNGNNNNN NNGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG NTCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTACGCGTA
            121 GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG GCCCTCTAGA
            181 CTCGACACTA GTTCACTGGG TGTGGAAATA GATGGGCTTG ACTTTCCCAG AAAGGATCTT
            241 GGGCACTTGC ACAGAGATGA TCTCTGCCAT CATTTCCGGA AAGTCCACGC TCACCATGTG
            301 TGACTTGATT AGCAGGTCAA AAGTGAACTG ATGCAGCTCT CTCGCAATAG GCTGCACGGA
            361 GTCCAGGAGC TTGGTGAGCT GGTAGAAGCG TCTTGAGCAG GATGTGGGAT TTTTTCTTTT
            421 GCATGCAATG ATACGATCGA GTTCCTTGAT GTAGTTCATT CGAAGTTCAT CAAAGAATTT
            481 TTGATTTTTC AGCCCATCCA CTGGAATAAT GCTGAAGAGT AGCAGTGCTT TCATGCACAG
            541 GAATTCCTGG GGGGTGATTT GGAGCCATCC AAACTCTTGA GAGAGGTGCC TCATTCGGAC
            601 ACACTGGCTG TACATCCGGG ACTTGTGCAT GCGGTACTCA TTGAAAACCA GATCAGGGGC
            661 GAAGTAGAGC ATCCTGGAGT TGACATTGGT GAAGGATCGC CAGCCCATGG CAAACACCAT
            721 GAGCCCCATC CAGGANTACT GAATGACAGC CATCTGGTCG TCCACGTGTA AGTTGCGGAA
            781 GCCAGGCAAG GCCTTGGCCC ACTTGACCAC GTGTACNAGC TGTCTCTCTC CCAGTTCATT
            841 GANGCTAGAN AGCAAGGNTG CAAAGGANTC GGGCTGGNTG TTGTCNNGTC CAGCACACNC
            901 TACACCNNGC TNNATNNNTT NNNNGNNNTT CAGAAANANG GGNTGANNTT CNTANNNNTC
          961 NNNGTGNGAN NCTNNCAGNN NNNNGNNNNN NNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40pro_ori,NeoR
          Sequence file: RDB18676_B1Aqc.seq check
          >18676_18676_B1Aq_2_SV40pro_ori_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCC NCCTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
            121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
            721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG
            781 GNCGGCTGGG TGTGGCGGAN CGCTATCAGG ACATANCGNT GGCTANCCNT GATATTGCTG
            841 AAGANCTTGN CGGCNAATGG GNNTGANCGC TTNNTCNNGC TTTACGGNAT CNCCGCTCCC
            901 GANNCNCANC GNATNNNCNT NCNATCNNNT TCNTGANGAG TNNNNCTNAN CGGNNNTCNN
          961 GGGTNCNNNA AANGANCNAN NNANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shizu, R., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [link to RRC of NBRP]

          2024.03.26

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