Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL3-phPSA -430 to +12

Luciferase reporter vector of human prostate-specific antigen (PSA) promoter (region -430 to +12).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
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提供条件	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18677_B1Aqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665) Region: pause site,insert,luc 5' Sequence file: RDB18677_B1Aqa.seq
>D07007A9L_B1Aq_2_pGL4ins_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNNANNA GGCTGTCCCC AGTGCAAGTG CNNGTGCCAG    61 AACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGC TCGAGGAATA   121 AAAGTTCTAG TTTCTGGTCT CAGAGTGGTG CAGGGATCAG GGAGTCTCAC AATCTCCTGA   181 GTGCTGGTGT CTTAGGGCAC ACTGGGTCTT GGAGTGCAAA GGATCTAGGC ACGTGAGGCT   241 TTGTATGAAG AATCGGGGAT CGTACCCACC CCCTGTTTCT GTTTCATCCT GGGCGTGTCT   301 CCTCTGCCTT TGTCCCCTAG ATGAAGTCTC CATGAGCTAC AGGGCCTGGT GCATCCAGGG   361 TGATCTAGTA ATTGCAGAAC AGCAAGTACT AGCTCTCCCT CCCCTTCCAC AGCTCTGGGT   421 GTGGGAGGGG GTTGTCCAGC CTCCAGCAGC ATGGGGAGGG CCTTGGTCAG CCTCTGGGTG   481 CCAGCAGGGC AGGGGCGGAG TCCTGGGGAA TGAAGGTTTT ATAGGGCTCC TGGGGGAGGC   541 TCCCCAGCCC CAAGCTTGGC ATTCCGGTAC TGTTGGTAAA GCCACCATGG AAGACGCCAA   601 AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCGCTGGAA GATGGAACCG CTGGAGAGCA   661 ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT TTACAGATGC   721 ACATATCGAG GTGGACATCA CTTACGCTGA GTACTTCGAA ATGTCCGTTC GGNTGGCAGA   781 AGCTATGAAA CGATATGGGC TGAATACAAA TCACAGAATC GTCGTATGCA NTGAAAACTC   841 TCTTCAATTC TTTATGCCGG TGTTGGGCGC GTTATTTATC GNANTTGCNG TTGCGCCCGC   901 NAACGANATT TATAATGAAC GTGAATTGNT CNACAGTATN GNCATTTCGC ANCCTANCGT   961 GNNGTTNGTT TCCNAAAAGG GGTTGCAAAA AATTTTNAAN GTGCAAAAAA ANNTCCNNNT  1021 CATNCNAAAA ATTATNATCN NNNNNNCTAA AACNGNNNNA NNNNGGNNTT CNNNNNNNNN 1081 N //
Primer: Luc_R (Pr0146) Region: luc 5',insert,pause site,pA signal Sequence file: RDB18677_B1Aqb.seq
>D07007A9L_B1Aq_2_Luc_R_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNANN NNNNGNCGNN NCTCTTNNTA GCCTTATGCA GTTGCTCTCC    61 AGCGGTTCCA TCTTCCAGCG GATAGAATGG CGCCGGGCCT TTCTTTATGT TTTTGGCGTC   121 TTCCATGGTG GCTTTACCAA CAGTACCGGA ATGCCAAGCT TGGGGCTGGG GAGCCTCCCC   181 CAGGAGCCCT ATAAAACCTT CATTCCCCAG GACTCCGCCC CTGCCCTGCT GGCACCCAGA   241 GGCTGACCAA GGCCCTCCCC ATGCTGCTGG AGGCTGGACA ACCCCCTCCC ACACCCAGAG   301 CTGTGGAAGG GGAGGGAGAG CTAGTACTTG CTGTTCTGCA ATTACTAGAT CACCCTGGAT   361 GCACCAGGCC CTGTAGCTCA TGGAGACTTC ATCTAGGGGA CAAAGGCAGA GGAGACACGC   421 CCAGGATGAA ACAGAAACAG GGGGTGGGTA CGATCCCCGA TTCTTCATAC AAAGCCTCAC   481 GTGCCTAGAT CCTTTGCACT CCAAGACCCA GTGTGCCCTA AGACACCAGC ACTCAGGAGA   541 TTGTGAGACT CCCTGATCCC TGCACCACTC TGAGACCAGA AACTAGAACT TTTATTCCTC   601 GAGCCCGGGC TAGCACGCGT AAGAGCTCGG TACCTATCGA TAGAGAAATG TTCTGGCACC   661 TGCACTTGCA CTGGGGACAG CCTATTTTGC TAGTTTGTTT TGTTTCGTTT TGTTTTGATG   721 GAGAGCGTAT GTTAGTACTA TCGATTCACA CNAAAAACCN ACACACAGAT GTAATGAAAA   781 TAAAGATATT TTATTGCGGC CGCTCNNNGT ACCTCCCGTA CCTTAATATT ACTTACTTAT   841 CATGGTAGCT TGGGCTGNCG TAATAGCGAA NANGNCCGCA CCGATCGCCC TTNCCNACAG   901 TTGCNCANCC TGANTGGNGA ANGNNAAANT GTAAGCGTTA ATATTTTGNN NAAATTCGCG   961 TTAANTTTTG TTAAATCANC TCATTTTTTN ANNNNNNNGN CNAANNNGGN AAANNCCTTN 1021 NNAANCNAAA NNNNNNNCGA NANANGNNNN NNNNNN //
Primer: SV40pA_R (Pr0882) Region: luc 3' Sequence file: RDB18677_B1Aqc.seq
>D07007A9L_B1Aq_2_SV40pA_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTT TNNNNNNTAA AGCATTTTTT TCNCTGCATT CTAGTTGTGG    61 TTTGTCCAAA NNNNNNNNNN NNNCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCNAN   121 TCTAGAATTA CACGGCGATC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT   181 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC   241 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG   301 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT   361 CGGGAAGACC TGCGACACCT GCGTCGAAGA TGTTGGGGTG TTGGAGCAAG ATGGATTCCA   421 ATTCAGCGGG AGCCACCTGA TAGCCTTTGT ACTTAATCAG AGACTTCAGG CGGTCAACGA   481 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT   541 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGAC   601 CTCTCACACA CAGTTCGCCT CTTTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA   661 CAACCTTCGC TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT   721 CGGGTGTAAT CAGAATAGCT GANGTAGTCT CAGTGAGCCC ATATCCTTGC CTGATACCTG   781 NCAGANGGAA CCTCTTGGCA ACCGCTTCCN CGANTTCCTT ANAGANGGGA NCGCCACCAG   841 AAGCAATTTC GTGTAAATTA NATAAATCGT ANTTGTCAAT CANANTGNTT TTGGCNAANA   901 ANGGANAATA GGGNTGNNNN CAGCANCGCN CTTTGAATCT NGTAATCCTG NANNNNNNCT   961 CANAAACNNN NCTTCTTCAA ATCTATACNN NAANANNANT CNAANTCCNN NTATNNNNAT 1021 NCNNANNGNA NNAAANNTNN CNNAANCNNG ANNGNNNNGN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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