Resource data sheet
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pEGFP-GR (#RDB18680)

Expression vector of human glucocorticoid receptor (GR), CMV promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Shizu, R., Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human glucocorticoid receptor (GR), CMV promoter.
Vector backbone pEGFP-c1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human NR3C1 cDNA
Depositor|Developer Negishi, Masahiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.

Catalog # Resource name Shipping form Fee
RDB18680 pEGFP-GR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEGFP-GR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18680).

Reference section:

Shizu, R., Yokobori, K., Perera, L., Pedersen, L., Negishi, M., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shizu, R., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [PubMed] [Article] [RRC of NBRP]