AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.
Clone info. | AAV2 expression vector of second generation tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter (human synapsin I (SYN)). The ITRs (inverted terminal repeats) are derived from AAV serotype 2. |
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Comment | Expression was confirmed by the depositor, co-transfection to cultured cell with a plasmid vector that expresses fluorescent protein(s) under TRE promoter. |
Vector backbone | plasmid |
Selectable markers | Ampicillin |
Growth conditions | LB+Amp, 37oC |
Depositor|Developer | Miyawaki, Atsushi | Hioki, Hiroyuki | Katayama, Hiroyuki | |
Sequence (full) ![]() |
RDB18685zzk01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Sohn, J. et al., PLoS One, 12 (1): e0169611 (2017). Hioki, H. et al., Neurosci. Res., 63 (2): 149-154 (2009). Katayama et al. Cell 181, 1176–1187 (2020). |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Sohn, J. et al., PLoS One, 12 (1): e0169611 (2017). Hioki, H. et al., Neurosci. Res., 63 (2): 149-154 (2009). Katayama et al. Cell 181, 1176–1187 (2020). |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18685 | pAAV2-SYN-tTAad-BGHpA | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pAAV2-SYN-tTAad-BGHpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18685). |
Reference section:
Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18685_B1B5p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: tTA-Ad R (Pr0542) Region: tTA-Advanced 5', h synapsin pro, attB2, AAV2 L-ITR Sequence file: RDB18685_B1B5a.seq ![]() |
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>D07612A2_B1B5_1_tTA-Ad R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNCTCTTGT CCNGTCNAGA NATGGTGAAT 61 TCGATATCAA GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC 121 TATCTCGCGC CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG 181 GTGGCCGGGA AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT 241 GAGGCGGCGG TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC 301 TGGCAGTGCG CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGGATG 361 CGCAATTTGG GGAATGGGGG TTGGGTGCTT GTCCAGTGGG TCGGGGTCGG TCGTCAGGTA 421 GGCACCCCCA CCCCGCCTCA TCCTGGTCCT AAAACCCACT TGCACTCATA CGCAGGGCCC 481 TCTGCAGGAC CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTA CGCGTGCGGC 541 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTN // |
Primer: BGHpA_F (Pr0373) Region: bGH pA, attB1, AAV2R-ITR Sequence file: RDB18685_B1B5b.seq ![]() |
>D07612A2_B1B5_1_BGHpA_F_H05_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGG GCANGANAGC AAGGGGGAGG 61 NNTGGGAAGA NAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGGC GGCCGCGAAT 121 TCGGTACCGG ATCCAGTCGA CTGAATTGGT TCCTTTAAAG CCTGCTTTTT TGTACAAACT 181 TGTCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT 241 CTGCGCGN // |
Primer: attB2-R1 (Pr0411) Region: h synapsin pro, tTA-Advanced 5' Sequence file: RDB18685_B1B5c.seq ![]() |
>D07612A2_B1B5_1_attB2-R1_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCG NATGAGNGNN NNTGGGTTTT AGGANCAGGA 61 TGAGGCGGGG TGGGGGTGCC TACCTGACGA CCGACCCCGA CCCACTGGAC AAGCACCCAA 121 CCCCCATTCC CCAAATTGCG CATCCCCTAT CAGAGAGGGG GAGGGGAAAC AGGATGCGGC 181 GAGGCGCGTG CGCACTGCCA GCTTCAGCAC CGCGGACAGT GCCTTCGCCC CCGCCTGGCG 241 GCGCGCGCCA CCGCCGCCTC AGCACTGAAG GCGCGCTGAC GTCACTCGCC GGTCCCCCGC 301 AAACTCCCCT TCCCGGCCAC CTTGGTCGCG TCCGCGCCGC CGCCGGCCCA GCCGGACCGC 361 ACCACGCGAG GCGCGAGATA GGGGGGCACG GGCGCGACCA TCTGCGCTGC GGCGGACGGT 421 ATCGATAAGC TTGATATCGA ATTCACCATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC 481 TCTGCTCTGG AATTACTCAA TGAAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT 541 CAAAAGCTGG GAGTTGAGCA GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG 601 CTCGATGCCC TGGCAATCGA GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA 661 GGCGAGTCAT GGCAAGACTT TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC 721 TCACATCGCG ACGGGGCTAA AGTGCATCTC NGCACCCGCC CAACAGAGAA ACAGTACGAA 781 ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT CAGCNANGCT TCTCCCTGGA GAACGCACTG 841 TACGCTCTGT CCGCCGTGNG CCACTTTACA CTGGGCTGCG NATTGNAGGA TCNGGANCNT 901 CAAGTANNNA AAGANNNAAG ANAGANNNCT NNNNNNGANT NCNATGCCCC CACTTCTGAG 961 ANANGCNANT NGAGCTGNNC GANNNNNNNN GNNNCCNNNN NNNNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA, tTA-Advanced 3' Sequence file: RDB18685_B1B5d.seq ![]() |
>D07612A2_B1B5_1_bGH_rev3_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCANNNNN NNNNNGCTGG CAACTAGAAG 61 GCACAGACTA GTGGATCCTT ACTTAGTTAC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA 121 AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG 181 TCTAAGTCAA AATCGTCAAG GGCGTCGGCC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC 241 TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGATGG 301 TCGAACAGCT CAATTGCTTG TCTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT 361 CTTTCCTCTT TTGCTACTTG ATGCTCCTGA TCCTCCAATA CGCAGCCCAG TGTAAAGTGG 421 CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC 481 GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT 541 TTAGCCCCGT CGCGATGTGA GAGGAGAGCA CAGCGGAATG ACTTGGCGTT GTTCCGCAGA 601 AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGAAGTGGG TATGATGCCT GTCCAGCATC 661 TCGATTGCCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC 721 TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTTCA 781 TTGAGTAATT CCNGANCANA NTTTATGACT TTGCTCTTGT CCAGTCTAGA CATGGTGAAT 841 TCNATATCAA GCTTATCGAT NCCGNCCGNN NCANCGCANA NGGTCGCGCC CGTGCCCCCC 901 TATCNCGCGC CTCGCGTGGT GNNGNNCNGG NTGGNNCNGC GNNGGCGCGG ANNNNANCAA 961 GGNGNCCNGG AAGGGGANTT TGCGGGGGNA NNNNCNANNG NNGTCAGCNC NNCTNCANNG 1021 NTNNNNNNNN CNNNNGNNCN NNNNNNNCNN NNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP] |
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