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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-SYN-tTAad-BGHpA (#RDB18685)

AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.

Clone info. AAV2 expression vector of second generation tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter (human synapsin I (SYN)). The ITRs (inverted terminal repeats) are derived from AAV serotype 2.
Comment Expression was confirmed by the depositor, co-transfection to cultured cell with a plasmid vector that expresses fluorescent protein(s) under TRE promoter.
Vector backbone plasmid
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Depositor|Developer Miyawaki, Atsushi | Hioki, Hiroyuki | Katayama, Hiroyuki |
 
Sequence (full) RDB18685zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Sohn, J. et al., PLoS One, 12 (1): e0169611 (2017). Hioki, H. et al., Neurosci. Res., 63 (2): 149-154 (2009). Katayama et al. Cell 181, 1176–1187 (2020).
Remarks Remember that you will be working with samples containing infectious virus.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Sohn, J. et al., PLoS One, 12 (1): e0169611 (2017). Hioki, H. et al., Neurosci. Res., 63 (2): 149-154 (2009). Katayama et al. Cell 181, 1176–1187 (2020).
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18685 pAAV2-SYN-tTAad-BGHpA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAAV2-SYN-tTAad-BGHpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18685).

Reference section:

Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18685_B1B5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: tTA-Ad R (Pr0542)
Region: tTA-Advanced 5', h synapsin pro, attB2, AAV2 L-ITR
Sequence file: RDB18685_B1B5a.seq check
>D07612A2_B1B5_1_tTA-Ad R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNN NNNCTCTTGT CCNGTCNAGA NATGGTGAAT
   61 TCGATATCAA GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC
  121 TATCTCGCGC CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG
  181 GTGGCCGGGA AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT
  241 GAGGCGGCGG TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC
  301 TGGCAGTGCG CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGGATG
  361 CGCAATTTGG GGAATGGGGG TTGGGTGCTT GTCCAGTGGG TCGGGGTCGG TCGTCAGGTA
  421 GGCACCCCCA CCCCGCCTCA TCCTGGTCCT AAAACCCACT TGCACTCATA CGCAGGGCCC
  481 TCTGCAGGAC CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTA CGCGTGCGGC
541 CGCAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTN
//
Primer: BGHpA_F (Pr0373)
Region: bGH pA, attB1, AAV2R-ITR
Sequence file: RDB18685_B1B5b.seq check
>D07612A2_B1B5_1_BGHpA_F_H05_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGGG GCANGANAGC AAGGGGGAGG
   61 NNTGGGAAGA NAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGGC GGCCGCGAAT
  121 TCGGTACCGG ATCCAGTCGA CTGAATTGGT TCCTTTAAAG CCTGCTTTTT TGTACAAACT
  181 TGTCACGTGC GGACCGAGCG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT
241 CTGCGCGN
//
Primer: attB2-R1 (Pr0411)
Region: h synapsin pro, tTA-Advanced 5'
Sequence file: RDB18685_B1B5c.seq check
>D07612A2_B1B5_1_attB2-R1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCG NATGAGNGNN NNTGGGTTTT AGGANCAGGA
   61 TGAGGCGGGG TGGGGGTGCC TACCTGACGA CCGACCCCGA CCCACTGGAC AAGCACCCAA
  121 CCCCCATTCC CCAAATTGCG CATCCCCTAT CAGAGAGGGG GAGGGGAAAC AGGATGCGGC
  181 GAGGCGCGTG CGCACTGCCA GCTTCAGCAC CGCGGACAGT GCCTTCGCCC CCGCCTGGCG
  241 GCGCGCGCCA CCGCCGCCTC AGCACTGAAG GCGCGCTGAC GTCACTCGCC GGTCCCCCGC
  301 AAACTCCCCT TCCCGGCCAC CTTGGTCGCG TCCGCGCCGC CGCCGGCCCA GCCGGACCGC
  361 ACCACGCGAG GCGCGAGATA GGGGGGCACG GGCGCGACCA TCTGCGCTGC GGCGGACGGT
  421 ATCGATAAGC TTGATATCGA ATTCACCATG TCTAGACTGG ACAAGAGCAA AGTCATAAAC
  481 TCTGCTCTGG AATTACTCAA TGAAGTCGGT ATCGAAGGCC TGACGACAAG GAAACTCGCT
  541 CAAAAGCTGG GAGTTGAGCA GCCTACCCTG TACTGGCACG TGAAGAACAA GCGGGCCCTG
  601 CTCGATGCCC TGGCAATCGA GATGCTGGAC AGGCATCATA CCCACTTCTG CCCCCTGGAA
  661 GGCGAGTCAT GGCAAGACTT TCTGCGGAAC AACGCCAAGT CATTCCGCTG TGCTCTCCTC
  721 TCACATCGCG ACGGGGCTAA AGTGCATCTC NGCACCCGCC CAACAGAGAA ACAGTACGAA
  781 ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT CAGCNANGCT TCTCCCTGGA GAACGCACTG
  841 TACGCTCTGT CCGCCGTGNG CCACTTTACA CTGGGCTGCG NATTGNAGGA TCNGGANCNT
  901 CAAGTANNNA AAGANNNAAG ANAGANNNCT NNNNNNGANT NCNATGCCCC CACTTCTGAG
961 ANANGCNANT NGAGCTGNNC GANNNNNNNN GNNNCCNNNN NNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA, tTA-Advanced 3'
Sequence file: RDB18685_B1B5d.seq check
>D07612A2_B1B5_1_bGH_rev3_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGGCANNNNN NNNNNGCTGG CAACTAGAAG
   61 GCACAGACTA GTGGATCCTT ACTTAGTTAC CCGGGGAGCA TGTCAAGGTC AAAATCGTCA
  121 AGAGCGTCAG CAGGCAGCAT ATCAAGGTCA AAGTCGTCAA GGGCATCGGC TGGGAGCATG
  181 TCTAAGTCAA AATCGTCAAG GGCGTCGGCC GGCCCGCCGC TTTCGCACTT TAGCTGTTTC
  241 TCCAGGCCAC ATATGATTAG TTCCAGGCCG AAAAGGAAGG CAGGTTCGGC TCCCTGATGG
  301 TCGAACAGCT CAATTGCTTG TCTCAGAAGT GGGGGCATAG AATCGGTGGT AGGTGTCTCT
  361 CTTTCCTCTT TTGCTACTTG ATGCTCCTGA TCCTCCAATA CGCAGCCCAG TGTAAAGTGG
  421 CCCACGGCGG ACAGAGCGTA CAGTGCGTTC TCCAGGGAGA AGCCTTGCTG ACACAGGAAC
  481 GCGAGCTGAT TTTCCAGGGT TTCGTACTGT TTCTCTGTTG GGCGGGTGCC GAGATGCACT
  541 TTAGCCCCGT CGCGATGTGA GAGGAGAGCA CAGCGGAATG ACTTGGCGTT GTTCCGCAGA
  601 AAGTCTTGCC ATGACTCGCC TTCCAGGGGG CAGAAGTGGG TATGATGCCT GTCCAGCATC
  661 TCGATTGCCA GGGCATCGAG CAGGGCCCGC TTGTTCTTCA CGTGCCAGTA CAGGGTAGGC
  721 TGCTCAACTC CCAGCTTTTG AGCGAGTTTC CTTGTCGTCA GGCCTTCGAT ACCGACTTCA
  781 TTGAGTAATT CCNGANCANA NTTTATGACT TTGCTCTTGT CCAGTCTAGA CATGGTGAAT
  841 TCNATATCAA GCTTATCGAT NCCGNCCGNN NCANCGCANA NGGTCGCGCC CGTGCCCCCC
  901 TATCNCGCGC CTCGCGTGGT GNNGNNCNGG NTGGNNCNGC GNNGGCGCGG ANNNNANCAA
  961 GGNGNCCNGG AAGGGGANTT TGCGGGGGNA NNNNCNANNG NNGTCAGCNC NNCTNCANNG
1021 NTNNNNNNNN CNNNNGNNCN NNNNNNNCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Katayama, H., Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration. Cell 181(5): 1176-1187 (2020). PMID 32437660. [link to RRC of NBRP]