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pGBD-C1-Rab22A-deltaCys-N118I (#RDB18871)

Expression vector of mouse Rab22A constitutive negative mutant (N118I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.

Clone info. Expression vector of mouse Rab22A constitutive negative mutant (N118I) lacking a C-terminal geranylgeranylation site (delta Cys) for yeast two-hybrid assay.
Comment Expression was confirmed by the depositor with yeast-two hybrid assay.
Vector backbone pGBD-C1 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), TRP1 (yeast)
Gene/insert name mouse Rab22a cDNA
Reference of insert sequence AB232616 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Itoh, T. et al., Genes Cells, 11 (9): 1023-1037, 2006); (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18871 pGBD-C1-Rab22A-deltaCys-N118I Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGBD-C1-Rab22A-deltaCys-N118I was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18871).

Reference section:

Osaki, F., Matsui, T., Hiragi, S., Homma, Y., Fukuda, M., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Osaki, F., RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11. J. Cell Sci. 134 (7): jcs257311 (2021). PMID 33712449. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]