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pEF-MFG-E8/E1E2PT/Flag

Mammalian expression vector of the long form, signal sequence, E1, E2 and PT domains of mouse Mfge8 (MFG-E8) tagged with FLAG at C-terminus.

Catalog number RDB18993
Resource name pEF-MFG-E8/E1E2PT/Flag
Clone info. Mammalian expression vector of the long form, signal sequence, E1, E2 and PT domains of mouse milk fat globule EGF and factor V/VIII domain containing (Mfge8, Aliases: MFG-E8) tagged with FLAG at C-terminus. See Fig.3a of Hanayama, R. et al. Nature, 417 (6885): 182-187, 2002.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pEF-BOS-EX (plasmid)
Size of vector backbone 5.3 kb
Selectable markers Ampicillin
Gene/insert name mouse Mfge8 cDNA
Depositor|Developer Nagata, Shigekazu |
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External Database
mouse Mfge8

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Hanayama, R. et al., Nature, 417 (6885): 182-187, 2002). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hanayama, R. et al., Nature, 417 (6885): 182-187, 2002)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
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          RDB18993 pEF-MFG-E8/E1E2PT/Flag Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-MFG-E8/E1E2PT/Flag was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18993).

          Reference section:

          Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., Nagata, S., Identification of a factor that links apoptotic cells to phagocytes. Nature 417 (6885): 182-187 (2002). PMID 12000961. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

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          References

          Original, user report and related articles

          original Hanayama, R., Identification of a factor that links apoptotic cells to phagocytes. Nature 417 (6885): 182-187 (2002). PMID 12000961. [link to RRC of NBRP]

          2023.10.05

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